Method for relieving pesticide stress of pseudo-ginseng and reducing pesticide residues by brassinolide

A technology of brassinolide and Panax notoginseng, applied in the directions of botanical equipment and methods, chemicals for biological control, plant growth regulators, etc. and export, affecting the quality and safety of Panax notoginseng, etc., to achieve the effect of reducing pesticide residues, alleviating the inhibition of photosynthesis, and protecting the efficacy of drugs

Active Publication Date: 2021-12-24
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] It takes at least 3 years from planting to harvesting. During production, shade sheds are usually used to create a shady environment. During this period, it is very easy to be infected with various diseases and insect pests such as fungi, bacteria, and nematodes. It is mainly controlled by the application of pesticides. diseases and insect pests, but the number of registered pesticides in Panax notoginseng is relatively small, and farmers in production often spray pesticides according to their own experience
Excessive use of pesticides and unscientific application methods will cause coercion of Sanqi itself and affect the efficacy of Sanqi. The quality and safety of notoginseng is also the main factor restricting the development and export of notoginseng industry
[0003] Brassinolide is considered to be the sixth largest type of plant endogenous hormone that regulates plant growth and development. As a steroidal plant hormone, it can cooperate with other plant hormones to participate in plant growth and development. At present, brassinosteroids There is no report on the application of relieving the stress of Sanqi pesticides and reducing the residues of pesticides in Panax notoginseng

Method used

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  • Method for relieving pesticide stress of pseudo-ginseng and reducing pesticide residues by brassinolide
  • Method for relieving pesticide stress of pseudo-ginseng and reducing pesticide residues by brassinolide
  • Method for relieving pesticide stress of pseudo-ginseng and reducing pesticide residues by brassinolide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Application of brassinosteroids in alleviating the stress of Panax notoginseng pesticides and reducing pesticide residues

[0027] (1) Select three-year-old Panax notoginseng with consistent growth and good growth conditions, randomly divide them into control group and treatment group, and transplant them into soil without pesticide use background, and cultivate them for one week;

[0028] (2) Dissolve the brassinosteroid powder with absolute ethanol, and then dilute it with pure water into 0μmol / L, 0.02μmol / L, 0.1μmol / L, 0.5μmol / L and 1μmol / L brassinosteroid solutions, and then Spray on the leaves of Panax notoginseng, and the standard is to wet the leaves without forming water droplets;

[0029] (3) 24 hours after spraying brassinosteroid, spray 2.4mL / L propiconazole solution on the leaves, and the leaves should be sprayed wet, but no water droplets will slide off as the standard;

[0030] (4) Five days after spraying pesticides, collect leaves of Panax no...

Embodiment 2

[0032] Example 2: Determination of Indexes Related to Antioxidative Enzyme Activity of Panax notoginseng

[0033] 1. Crude enzyme solution extraction

[0034] Take 0.2g of fresh Panax notoginseng leaves in a pre-cooled mortar, add 1.5mL of 50mmol / L pre-cooled phosphate buffer solution (pH7.8) three times, grind it on ice to form a homogenate, and quickly transfer to the centrifuge Centrifuge the tube at 4°C and 12000g for 20min, and the supernatant is the crude enzyme extract;

[0035] 2. Determination of SOD activity

[0036] Configuration of reaction solution: Take 2.7mL of 14.5mmol / L methionine solution, 0.1mL of 3mmol / L EDTA-Na2 solution, 0.1mL of 2.25mmol / L nitrogen blue tetrazolium solution, and 0.1mL of 6mmol / L riboflavin solution shake well;

[0037] Add 40 μL of enzyme solution to 3 mL of reaction solution, mix well and shake well without shading. Add 40 μL of phosphate buffer (pH7.8, 0.05M) to 3 mL of reaction solution without shading as the maximum photoreduction...

Embodiment 3

[0053] Example 3: Detection experiment of brassinosteroids on membrane lipid peroxidation and damage of Panax notoginseng leaves under pesticide stress

[0054] 1. Determination of MDA

[0055] Five days after spraying pesticides, the leaves of Panax notoginseng were collected and washed with deionized water. The leaves of Panax notoginseng were frozen in liquid nitrogen and placed in a -80°C refrigerator to prepare fresh samples of Panax notoginseng leaves; Thoroughly grind trichloroacetic acid, centrifuge the homogenate at 4000g for 10min, take 2mL of the extract, add 2mL, 6g / L thiobarbituric acid (prepared with 10% trichloroacetic acid by volume), boil in a boiling water bath for 15min, Rapidly cool down and centrifuge; measure the absorbance of the supernatant at 532nm and 450nm; replace the extract with 2mL of water in the control tube;

[0056] C b =6.45×A 532 -0.56×A 450

[0057] C b is the content of MDA, the unit is μmol / L;

[0058] see results Figure 5 , it ...

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Abstract

The invention discloses a method for relieving pesticide stress of pseudo-ginseng and reducing pesticide residues by brassinolide, which comprises the following steps of: before spraying pesticide to pseudo-ginseng, spraying 0.02-1mu mol / L brassinolide solution to leaf surfaces of pseudo-ginseng until the leaf surfaces are wet but water drops are not formed; the implementation result of the method shows that brassinolide can significantly improve the antioxidant enzyme activity and osmoregulation substance content of panax notoginseng leaves under pesticide stress, reduce malondialdehyde, relative conductivity and active oxygen content, improve photosynthesis, guarantee main effective components of panax notoginseng and reduce pesticide residues, and the method is low in cost and easy and convenient to operate and has important significance for promoting the sustainable development of the pseudo-ginseng industry in China.

Description

technical field [0001] The invention belongs to the technical field of Panax notoginseng planting and pesticide residue treatment, and specifically relates to the application of brassinosteroids in alleviating the stress reduction of Panax notoginseng pesticides and pesticide residues. Background technique [0002] It takes at least 3 years from planting to harvesting. During production, shade sheds are usually used to create a shady environment. During this period, it is very easy to be infected with various diseases and insect pests such as fungi, bacteria, and nematodes. It is mainly controlled by the application of pesticides. Pests and diseases, but the number of registered pesticides in Panax notoginseng is relatively small, and farmers in production often spray pesticides according to their own experience. Excessive use of pesticides and unscientific application methods will cause coercion of Sanqi itself and affect the efficacy of Sanqi. The quality and safety of no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/22A01N25/32A01G13/00A01P15/00A01P21/00
CPCA01N43/22A01N25/32A01G13/00Y02A50/30
Inventor 郑凯
Owner KUNMING UNIV OF SCI & TECH
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