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Preparation method and application of alcohol-dispelling oligomeric pectin

A technology for oligomeric pectin and hangover relief, which is applied in the directions of antidote, fermentation, drug combination, etc., can solve problems such as low grafting efficiency of products, and achieve the effects of improving comprehensive utilization rate, remarkable antioxidant capacity and good use effect.

Inactive Publication Date: 2021-12-24
广州融知技转一号投资合伙企业(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current grafting methods of flavonoids, the grafting efficiency of the product is low, which is not enough to meet the requirements of industrialization

Method used

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  • Preparation method and application of alcohol-dispelling oligomeric pectin
  • Preparation method and application of alcohol-dispelling oligomeric pectin
  • Preparation method and application of alcohol-dispelling oligomeric pectin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take 100g of natural sugar orange peel pectin in an Erlenmeyer flask, add 2.4L of distilled water, use citric acid to adjust the pH to 4.5, add pectin lyase to 1.5% of dry weight, and add cellulase to 0.8% of dry weight %, enzymatic hydrolysis for 80 min at 55°C; 2 o 2 To a concentration of 0.18M, ​​adjust the pH value to 5.0, then add 80g of quercetin, stir and react for 30min; the reaction solution is treated with a cellulose membrane with a molecular weight cut-off of 30ku and 10ku, and the filtrate with a molecular weight of 10-30ku is collected, and the filtration pressure is set to 0.12MPa; The filtrate was cooled to 6°C, precipitated with ethanol, and the precipitate was dialyzed for 28 hours using a dialysis bag with a molecular weight cut-off of 3ku, and dried to obtain oligomerized modified pectin.

Embodiment 2

[0030] Take 100g of natural sugar orange peel pectin in an Erlenmeyer flask, add 2.4L of distilled water, use citric acid to adjust the pH to 4.8, add pectin lyase to 0.5% of dry weight, and add cellulase to 1.0% of dry weight %, enzymatic hydrolysis for 100 min at 58°C; after enzymatic hydrolysis, add ascorbic acid to the enzymatic hydrolysis solution to a concentration of 2 o 2 To a concentration of 0.16M, adjust the pH value to 5.5, then add 80g of quercetin, stir and react for 45min; the reaction solution is treated with a cellulose membrane with a molecular weight cut-off of 30ku and 10ku, and the filtrate with a molecular weight of 10-30ku is collected, and the filtration pressure is set to 0.12MPa; The filtrate was cooled to 6°C, precipitated with ethanol, and the precipitate was dialyzed for 28 hours using a dialysis bag with a molecular weight cut-off of 3ku, and dried to obtain oligomerized modified pectin.

Embodiment 3

[0032] Take 100g of natural sugar orange peel pectin in an Erlenmeyer flask, add 2.4L of distilled water, use citric acid to adjust the pH to 5.0, add pectin lyase to 1.2% of dry weight, and add cellulase to 0.8% of dry weight , enzymatic hydrolysis at 60°C for 90 minutes; after the enzymatic hydrolysis, add ascorbic acid to the enzymatic hydrolysis solution to a concentration of 0.12M, H 2 o 2 To a concentration of 0.12M, adjust the pH value to 4.5, then add 80g of quercetin, stir and react for 45min; the reaction solution is treated with a cellulose membrane with a molecular weight cut-off of 30ku and 10ku, and the filtrate with a molecular weight of 10-30ku is collected, and the filtration pressure is set to 0.12MPa; The filtrate was cooled to 6°C, precipitated with ethanol, and the precipitate was dialyzed for 28 hours using a dialysis bag with a molecular weight cut-off of 3ku, and dried to obtain oligomerized modified pectin.

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Abstract

The invention discloses a preparation method of alcohol-dispelling oligomeric pectin, and belongs to a production process of macromolecular pectin. The preparation method comprises the following steps of: enzymolysis, grafting, separation and purification. According to the preparation method, natural granulated sugar orange peel pectin is treated by adopting pectin lyase and cellulase, the pectin is split to a target molecular weight, flavonoid substances are grafted onto the pectin by a free radical-mediated graft copolymerization method, then filtrate with a molecular weight of 10 to 30ku is collected by membrane filtration, and the filtrate is subjected to alcohol precipitation, dialysis and drying to obtain oligomeric modified pectin with a molecular weight of 10 to 30ku. The oligomeric modified pectin obtained by the method has high antioxidant activity, can form a layer of protective film in the gastrointestinal tract to hinder the absorption of alcohol and accelerate the decomposition of alcohol in blood, and can be applied to the research and development of alcohol-dispelling products.

Description

technical field [0001] The invention relates to a method for preparing anti-alcohol oligomerization pectin, which belongs to the technical field of high-molecular pectin production. Background technique [0002] Pectin is a natural polysaccharide polymer with good gelling, stabilizing, emulsifying and thickening functions, and is generally used as an additive in the food industry. However, the molecular weight of natural polymer pectin is between 50-300ku, poor water solubility, difficult to absorb and other shortcomings, and its application in biological activity is limited. The molecular weight of oligomeric pectin is generally between 5-30ku. It has strong antioxidant properties, is easy to dissolve, and can be directly absorbed into the blood circulation to exert pharmacological effects. Studies have found that natural oligomeric pectin can effectively alleviate drunkenness. Finally, it can form a protective film in the gastrointestinal tract to hinder the absorption of...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C08B37/06A61P39/02
CPCC12P19/14C12P19/04C08B37/0045A61P39/02
Inventor 陈进林陈文勇莫树平
Owner 广州融知技转一号投资合伙企业(有限合伙)
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