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Method for rapidly detecting content of cyanide in nuts

A detection method, cyanide technology, applied in the direction of material analysis, measurement device, test sample preparation, etc. by observing the influence of chemical indicators, can solve the problems of high cost, complex processing, difficult batch operation, etc., to achieve Low equipment requirements, simple operation, and improved analysis efficiency

Active Publication Date: 2021-12-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Existing methods for detecting cyanide in nuts are complex, costly and difficult to operate in batches

Method used

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  • Method for rapidly detecting content of cyanide in nuts
  • Method for rapidly detecting content of cyanide in nuts
  • Method for rapidly detecting content of cyanide in nuts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Utilizes QuEChERS pre-treatment technology to process nuts

[0046] Nut samples were ground and crushed through a 425μm standard sieve, accurately weighed 5g of nut powder into a 50mL plastic centrifuge tube, added 10mL of water, vortexed and mixed, soaked for 30min, added 15mL of acetonitrile, ultrasonicated for 15min, frozen at -20°C for 8min, and placed in a 6 ceramic homogenizers, add 6.0g MgSO 4 , 1.5g NaOAc, shake and vortex immediately for 1min, then centrifuge at 5000r / min for 5min, draw (8mL) the supernatant until it contains 1200mg MgSO 4 , 400mg C 18 and 400mg GCB in a 15mL plastic centrifuge tube, vortex for 1min, centrifuge at 5000r / min for 5min, pipette 1mL of the supernatant into a 5mL EP tube, blow with nitrogen until nearly dry, add 5mL of 2g / L NaOH for colorimetric and spectroscopic analysis.

Embodiment 2

[0047] Example 2 Using QuEChERS pretreatment technology to process nuts

[0048] Nut samples were ground and crushed through a 425 μm standard sieve, accurately weighed 2 g of nut powder into a 50 mL plastic centrifuge tube, added 4 mL of water, vortexed and mixed, soaked for 15 min, added 6 mL of acetonitrile, ultrasonicated for 8 min, frozen at -20 °C for 8 min, and placed in a ceramic homogeneous, add 2.4g MgSO 4 , 0.6g NaOAc, shake and vortex immediately for 1min, then centrifuge at 5000r / min for 5min, draw (2mL) the supernatant to contain 300mg MgSO 4 , 100mg C 18 and 100mg GCB in a 15mL plastic centrifuge tube, vortex for 1min, centrifuge at 5000r / min for 5min, pipette 1mL supernatant into a 5mL EP tube, blow with nitrogen until nearly dry, add 5mL 2g / L NaOH for colorimetric and spectroscopic analysis.

Embodiment 3

[0049] The detection method of cyanide content in the fruit of embodiment 3

[0050] (1) Solution preparation: Use GBW(E)080115, the standard material for cyanide component analysis in water, to prepare cyanide ion standard intermediate solution (1μg / mL); sodium hydroxide solution (2g / L, 20g / L); phenolphthalein solution (10g / mL); L); Acetic acid solution (1+24): Mix acetic acid and water at a volume ratio of 1:24; Chloramine T solution (10g / L): Dissolve 1g of Chloramine T in water and dilute to 100mL. Prepare just before use; phosphate buffer (0.5M, pH=7); isonicotinic acid-barbituric acid reagent: weigh 2.5g of isonicotinic acid (C 5 h 5 o 2 N) and 1.25g barbituric acid (C 4 h 4 N 2 o 3 ), add to 100mL sodium hydroxide (20g / L) solution at 60℃~70℃, stir until dissolved, add water to 100mL after cooling, store in refrigerator at 4℃.

[0051] (2) QuEChERS pre-treatment of raw materials: Grind the nut samples and pass through a 425μm standard sieve, accurately weigh 5g of ...

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Abstract

The invention discloses a method for rapidly detecting the content of cyanide in nuts, which belongs to the technical field of food analysis and detection. According to the method, a buffer salt system and the formula of an extracting agent and an adsorbent for QuEChERS pretreatment are optimized, spectrophotometric method detection is combined, reaction with chloramine T is carried out, CN<-> is converted into cyanogen chloride, then chromogenic reaction with isonicotinic acid-barbituric acid is carried out to generate the purple blue dye, absorbance is measured by using a spectrophotometer, and the cyanide content in the sample isquantitatively obtained by combining a standard curve method. The detection method established by the invention can realize low-sample-amount rapid batch sample pretreatment, has low requirements on instruments and equipment, can efficiently improve the detection efficiency, and has the advantages of rapidness, economy and wide applicability.

Description

technical field [0001] The invention relates to a rapid detection method for cyanide content in nuts, belonging to the technical field of food analysis and detection. Background technique [0002] Cyanide is a common class of highly toxic compounds. Among all cyanides, hydrogen cyanide is the most toxic and can enter the human body through oral administration, inhalation, injection, and skin and mucous membrane absorption. CN - It can inhibit the activity of cytochrome C oxidase, block the respiratory chain and cause tissue hypoxia, and then produce a large amount of acidic substances such as lactic acid, eventually leading to metabolic acidosis and causing a series of nervous system diseases in the human body. [0003] Nuts are currently of increasing commercial value in the food, cosmetic and pharmaceutical sectors. However, nuts contain cyanogenic glycosides that can be hydrolyzed to produce highly toxic substances, which is an important hidden danger to the quality and...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78G01N1/28
CPCG01N21/31G01N21/78G01N1/286G01N2001/2866
Inventor 陈双徐岩朱晓春
Owner JIANGNAN UNIV
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