Biological analysis method for clinically researching concentration of chlorpromazine and metabolite in plasma sample

A technology for biological analysis and chlorpromazine, which is applied in the field of biological analysis of chlorpromazine and metabolite concentrations in clinical research plasma samples, can solve problems such as excessive plasma matrix interference, and achieve accurate and reliable results, high sensitivity and small dosage. Effect

Pending Publication Date: 2022-01-11
HUNAN HUIZE BIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention adopts the methanol protein precipitation method to confirm that the first-selected isotope chlorpromazine hydrochloride- d 6 and 7-hydroxychlorpromazine- d 6 As an internal standard, it effectively solves the problem of excessive plasma matrix interference, greatly improves the detection efficiency, high sensitivity, and accurate and reliable results

Method used

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  • Biological analysis method for clinically researching concentration of chlorpromazine and metabolite in plasma sample
  • Biological analysis method for clinically researching concentration of chlorpromazine and metabolite in plasma sample
  • Biological analysis method for clinically researching concentration of chlorpromazine and metabolite in plasma sample

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Experimental program
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Effect test

Embodiment 1

[0076] 1. Solution preparation

[0077]Mobile phase A (5 mM ammonium acetate-0.1% formic acid aqueous solution): Weigh 385.4 mg of ammonium acetate into 1L of ultrapure water, add 1 mL of formic acid, mix well, and sonicate.

[0078] Mobile phase B (0.2% formic acid in acetonitrile): Measure 1L of acetonitrile (Merck) and transfer to an appropriate solvent bottle, add 2mL of formic acid, mix well, and sonicate.

[0079] Needle washing solution (50% methanol in water): transfer 500 mL of methanol and 500 mL of ultrapure water to appropriate solvent bottles, and mix well.

[0080] Diluted solution (50% methanol in water): transfer 500 mL of methanol and 500 mL of ultrapure water to appropriate solvent bottles, and mix well.

[0081] Precipitating agent (methanol): Measure 200 mL of methanol into an appropriate solvent bottle.

[0082] 2. Standard solution preparation:

[0083] Preparation of chlorpromazine (LBQ) standard reference stock solution (LBQ STD Stock, 1.000 mg / mL) ...

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Abstract

The invention belongs to the technical field of medicine detection, and particularly discloses a biological analysis method for clinically researching the concentration of chlorpromazine and metabolite in a plasma sample, and the concentration of chlorpromazine and metabolite 7-hydroxy chlorpromazine in human plasma is measured by adopting an LC-MS / MS method. Methanol protein precipitation is adopted to confirm that isotope chlorpromazine-d6 / 7-hydroxy chlorpromazine-d6 is the first choice as an internal standard, a problem of many plasma matrix interference is solved, the sensitivity is high, the result is accurate and reliable, the matrix effect, the hyperlipidemia matrix effect and the hemolysis matrix effect are not interfered, the accuracy and precision are high, the dosage is small, and the method can be used for clinical large-batch sample analysis.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a biological analysis method for the concentrations of chlorpromazine and metabolites in plasma samples for clinical research. Background technique [0002] Chlorpromazine hydrochloride tablets, the molecular formula is: C 17 h 19 ClN 2 S HCl; molecular weight: 355.33; chemical name: N, N-dimethyl-2-chloro-10H-phenothiazine-10-propylamine hydrochloride, is a phenothiazine antipsychotic, its mechanism of action It is mainly related to blocking the dopamine receptor (DA2) in the mesolimbic system and mesocortical pathway. It has blocking effects on dopamine (DA1) receptors, 5-hydroxytryptamine receptors, M-type acetylcholine receptors, and α-adrenoceptors, and has a wide range of effects. In addition, this product can inhibit the dopamine receptors in the emetic chemoreceptor area of ​​the medulla in small doses, and directly inhibit the vomiting center in large doses...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/86G01N27/62G01N1/38G01N1/34
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/8675G01N27/62G01N1/38G01N1/34G01N2030/324
Inventor 蒲姝瑾朱俊王徽龙俊宇尹小丽
Owner HUNAN HUIZE BIO PHARMA CO LTD
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