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Anticancer compound capable of targeting intestinal tumor as well as preparation method and application of anticancer compound

A technology of intestinal tumors and complexes, applied in the field of complexes, can solve the problems of high active oxygen efficiency, lack of tumor targeting and enrichment ability, and low efficiency, and achieve high production efficiency, strong clinical transformation potential, and wide application foreground effect

Pending Publication Date: 2022-01-14
SHANGHAI THERANOSTICS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these nanomaterials lack tumor targeting and enrichment capabilities when administered orally.
At the same time, the efficiency of these nanomaterials to generate active oxygen depends on the pH value in the microenvironment. The efficiency of active oxygen generation is high when it is weakly acidic, and the efficiency is low when it is weakly alkaline.

Method used

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  • Anticancer compound capable of targeting intestinal tumor as well as preparation method and application of anticancer compound
  • Anticancer compound capable of targeting intestinal tumor as well as preparation method and application of anticancer compound
  • Anticancer compound capable of targeting intestinal tumor as well as preparation method and application of anticancer compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1) Preparation of nanomaterials capable of generating ROS:

[0066] 1314mg 2-methylimidazole, 1190mg zinc nitrate hexahydrate, 140mg iron acetylacetonate and 20mg chloroauric acid were dissolved in 45mL methanol, ultrasonicated for 10min, and stirred at room temperature for 2 hours to obtain an organometallic framework material containing gold and iron (ZIF-8 / AuFe) , whose microscopic shape is as figure 2 shown;

[0067] 2) Modification of ROS-generating nanomaterials with linker molecules:

[0068] 50mL 1mg / mL ZIF-8 / AuFe and 50mL 1mg / mL C18-PEG-B were stirred for 2 hours at low temperature, centrifuged, washed, and re-dispersed in 50mL saline to obtain the nanomaterial ZIF modified by linker molecules -8 / AuFe@C18-PEG-B.

[0069] 3) Prepare the probiotic liquid:

[0070] Cultivate bifidobacteria (microscopic morphology such as image 3 (a) shown), collected by centrifugation and re-dispersed in saline to control its concentration to 1×10 7 CFU / mL.

[0071] 4) Prep...

Embodiment 2

[0076] The anticancer complex was prepared with reference to the method of Example 1, but the amount of chloroauric acid in step 1) was adjusted, specifically: 1314mg 2-methylimidazole, 1190mg zinc nitrate hexahydrate, 140mg iron acetylacetonate and 10mg chloroauric acid Dissolve in 45mL of methanol, sonicate for 10min, and stir at room temperature for 2 hours to obtain an organometallic framework material (ZIF-8 / AuFe) containing gold and iron. Other conditions remain unchanged, the final anti-cancer complex ZIF-8 / AuFe@C18-PEG-B@Probiotics (B) looks as follows under the transmission electron microscope Figure 5 The microscopic morphology shown.

Embodiment 3

[0078] The anticancer complex was prepared with reference to the method of Example 1, but the linker molecules used in step 2) were different, specifically: 50mL 1mg / mL ZIF-8 / AuFe and 50mL 1mg / mL 4-mercaptophenylboronic acid, in Stir for 2 hours at low temperature, centrifuge, wash, and redisperse in 50 mL of saline to obtain the nanomaterial ZIF-8 / AuFe@4-mercaptophenylboronic acid modified by linker molecules, and other conditions remain unchanged. The final anticancer complex ZIF-8 / AuFe@4-mercaptophenylboronic acid@Probiotics was shown as follows under the transmission electron microscope Image 6 The microscopic morphology shown.

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Abstract

The invention provides an anti-cancer compound capable of targeting intestinal tumors. The anti-cancer compound is formed by fixing a nano material on the surface of probiotics by connecting molecules,the nano material is selected from any one of a transition metal-containing composite nano material, a transition metal-containing nano metal oxide, a transition metal-containing nano metal peroxide, a transition metal-containing nano metal sulfide or a transition metal-containing nano metal material; one end of the connecting molecule is combined with the bacteria surface molecule, and the other end of the connecting molecule is combined with the nano material. According to the invention, the probiotics and the nanometer material capable of generating active oxygen are compounded, so that the enrichment of the nanometer material in the intestinal tract, especially the focus of the intestinal tumor, is remarkably improved, the capability of generating the active oxygen in the intestinal tract of the nanometer material is enhanced, and the treatment capability on the intestinal tumor is improved; the obtained compound can be used for preparing oral medicines for treating intestinal cancer, the preparation method is simple, convenient and controllable, the production efficiency is high, and the treatment effect is remarkable.

Description

technical field [0001] The invention relates to a compound with anti-tumor function, in particular to an anti-cancer compound capable of targeting intestinal tumors, its preparation method and application. Background technique [0002] Intestinal cancer, referred to as intestinal cancer, is a common malignant tumor in the gastrointestinal tract, and its incidence rate is second only to gastric and esophageal cancer. Colorectal cancer has the characteristics of insidious onset, often has no obvious clinical manifestations in the early stage, and often has reached the middle and late stages when it is discovered. It only takes 6 months from diagnosis to death in advanced colorectal cancer, with a high mortality rate and a low cure rate. The vast majority of patients with bowel cancer are over 40 years old, and about 15% are under 30 years old. With the development of population aging, the incidence of colon cancer is high and has been increasing year after year. According t...

Claims

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Application Information

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IPC IPC(8): A61K47/69
CPCA61K47/60A61K9/0053A61P35/00A61P37/04B82Y5/00B82Y30/00B82Y40/00A61K47/6901A61K33/242A61K33/26
Inventor 陈小元毛峥伟曹芳芳
Owner SHANGHAI THERANOSTICS BIOTECH CO LTD
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