Sensing platform based on ECL co-reactant accelerator, preparation method and application
A sensing platform and co-reactant technology, applied in the field of sensors and nano-functional materials, can solve the problems of unable to maintain three-dimensional structure and no application, etc., and achieve the effect of enhancing luminescence, reducing efficiency and enhancing stability
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Embodiment 1
[0054] A preparation method of a sensing platform based on an ECL co-reactant accelerator, such as figure 1 As shown, the following steps are included: (1) Preparation of hollow ZnSn(OH) 6 (H-ZnSn(OH) 6 )
[0055] Preparation of solid ZnSn(OH) by co-precipitation method at room temperature 6 (S-ZnSn(OH) 6 ) and then use high-concentration strong alkali etching method to prepare hollow ZnSn(OH) with slightly smooth water chestnut 6 (H-ZnSn(OH) 6 ), the specific implementation is: weigh 2 mmol of SnCl 4 • 5H 2 O was dissolved in 10 mL of absolute ethanol, and the above solution was added to 2 mmol of ZnCl 2 and 2 mmol of citric acid monohydrate in 20 mL of mixed aqueous solution, then magnetically stirred the solution for 10 min, then quickly added 50 mL of 0.5 mol L -1 NaOH solution to form a solid cubic solid, and then continue to stir for 10 min, then slowly add 20 mL of 2mol L in 4 min -1 NaOH solution, after stirring for 6 min, the precipitate was centrifuged, was...
Embodiment 2
[0073] Sensing and detecting protein application of functionalized complex based on co-reactant accelerator three-dimensional hollow tin sulfide
[0074] (1) Cleaning of glassy carbon electrode GCE and preparation of ECL immunosensor
[0075] Glassy carbon electrodes (GCE, Φ = 3 mm) were polished with 0.3 μm and 0.05 μm alumina powder slurries to obtain mirror-clean surfaces. Then, GCE was sonicated for 10 min each in ultrapure water, ethanol, and ultrapure water. Finally, the polished and clean electrode was dried with nitrogen flow for further use. Next, the construction process of the electrochemiluminescent immunosensor is as follows: figure 1 shown. 6 uL 3D H-SnS 2 - The APTES-PTCA suspension was dropped onto the clean glassy carbon electrode GCE. After drying at room temperature, soak the electrode in 0.5 mM HAuCl 4 In solution (0.1 M PBS, pH=7.4), electrodeposition was performed for 5 cycles by cyclic voltammetry (CV), the potential window was set at -0.2 ~ 0.8 V,...
Embodiment 3
[0084] In order to make the prepared electrochemiluminescent bioimmunosensor exhibit the best performance, the relevant parameters of the preparation process were optimized as follows.
[0085] In order to obtain the best experimental results, the pH value, K 2 S 2 o 8 concentration, 3D H-SnS 2 The concentration ratio to PTCA, the number of circles of electrodeposited gold nanoparticles, and the immunoreaction time of antibody anti-cTnI and antigen cTnI were optimized. First, if Figure 12 As shown in A, as the pH value increased from 6.5 to 8.0, the intensity of ECL has been increasing and decreased after 8.0, which may be due to the rapid reduction of protons suppressing the luminescence at low pH, while at high pH, SO4· - Easier with OH - Reaction, and high pH will also inhibit the activity of the protein, resulting in a decrease in ECL intensity. Therefore, pH 8.0 was chosen as the optimal condition for this experiment. Then, if Figure 12 As shown in B, optimize...
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