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Preparation method of gel microorganism composite microspheres combining chemical repairing agent and microorganisms

A technology of composite microspheres and chemical remediation, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as soil heavy metal pollution, achieve easy implementation, reduce heavy metal content, and passivation ability strong effect

Pending Publication Date: 2022-01-25
上海市农产品质量安全中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention restores the polluted soil with excessive heavy metal content through the adsorption and complexation of the polymer to the heavy metal and the adsorption of the filler to the heavy metal, and at the same time combines the in-situ bioremediation technology to solve the problem of soil heavy metal pollution

Method used

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  • Preparation method of gel microorganism composite microspheres combining chemical repairing agent and microorganisms
  • Preparation method of gel microorganism composite microspheres combining chemical repairing agent and microorganisms
  • Preparation method of gel microorganism composite microspheres combining chemical repairing agent and microorganisms

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preparation example Construction

[0026] The present invention provides a kind of preparation method of the gel microorganism composite microsphere that chemical restoration agent and microorganism combine, comprise the following steps:

[0027] S1. The inorganic filler and the gel monomer are polymerized into gel microspheres under the initiation of the initiator ammonium persulfate;

[0028] S2, the gel microspheres are dispersed in water after centrifugal purification, adjusted to PH to 12 with NaOH, freeze-dried to become gel microsphere powder;

[0029] S3. The gel microsphere powder is dissolved in the microbial liquid. Through the superabsorption of sodium carboxylate, a large amount of microbial liquid is absorbed to prepare a gel microbiological composite microsphere, and the chemical repair agent is realized through the gel microsphere and the microorganism. combination with microorganisms.

[0030] Preferably, the inorganic filler is at least one of diatomaceous earth, limestone and activated carbo...

Embodiment 1

[0037] First, the inorganic filler diatomite is ground through an 80-mesh sieve to obtain uniform diatomite particles. Diatomaceous earth was dissolved in 100m of water, and 2g of acrylic acid, 3g of N-methylolacrylamide, and 1g of styrene were added. Pass through N2 protection, add potassium persulfate initiator at 300r / min and 70°C, and polymerize for 8 hours to obtain gel microsphere polymerization solution. After centrifugal purification, adjust the pH to 12 with NaOH, centrifuge, and dry to obtain gel microspheres. Add 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl to 950 ml of deionized water, adjust the pH of the medium to 7.4 with NaOH, and then dilute to 1 L with deionized water. The above-mentioned LB medium was used for the cultivation of D10 rhizobia and W33 rhizobia as microorganisms, and a composite bacterial suspension was prepared after shaking at 150 rpm for 24 hours at 28°C. The gel microspheres and the composite bacterial suspension were mixed ac...

Embodiment 2

[0044] First, the inorganic filler diatomite is ground through an 80-mesh sieve to obtain uniform diatomite particles. Dissolve diatomaceous earth and limestone in 100m of water, add 2g of acrylic acid, 3g of N-methylolacrylamide, and 10g of methacrylate. Pass through N2 protection, add potassium persulfate initiator at 300r / min and 70°C, and polymerize for 8 hours to obtain gel microsphere polymerization solution. After centrifugal purification, adjust the pH to 12 with NaOH, centrifuge, and dry to obtain gel microspheres. Add 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl to 950 ml of deionized water, adjust the pH of the medium to 7.4 with NaOH, and then dilute to 1 L with deionized water. The above-mentioned LB medium was used for the cultivation of D10 rhizobia and W33 rhizobia as microorganisms, and a composite bacterial suspension was prepared after shaking at 150 rpm for 24 hours at 28°C. The gel microspheres and the composite bacterial suspension were mixe...

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Abstract

The invention discloses a preparation method of gel microorganism composite microspheres combining a chemical repairing agent and microorganisms. The preparation method comprises the following steps of: S1, polymerizing inorganic filler and a gel monomer under the initiation of an initiator ammonium persulfate to form gel microspheres; S2, centrifuging and purifying the gel microspheres and dispersing in water; adjusting the pH (potential of Hydrogen) to 12 by utilizing NaOH; freeze-drying to obtain gel microsphere powder; and S3, dissolving the gel microsphere powder into microorganism liquid; adsorbing a lot of the microorganism liquid through a super-water absorption effect of sodium carboxylate to prepare the gel microorganism composite microspheres. The chemical repairing agent is used for adsorbing and enriching polluted metal and the polluted metal is continuously degraded and converted by utilizing microorganism repairing, so that the enrichment and degradation harmless treatment of pollutants is realized and the removing efficiency of the metal pollutants is extremely improved. The preparation method disclosed by the invention has the advantages of simple technology, no pollution and easiness for implementation. The content of heavy metal in soil can be efficiently reduced through a synergistic effect with the microorganisms.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a preparation method of a gel microbial composite microsphere combined with a chemical restoration agent and microorganisms. Background technique [0002] Soil heavy metal pollution refers to human activities, the content of trace metal elements in the soil exceeds the background value, and the excessive content caused by excessive deposition is collectively referred to as soil heavy metal pollution. With the development of the economy, heavy metals are becoming more and more indispensable to the progress of science and technology and the development of the times. The pollution of heavy metals has become an environmental pollution problem that developed countries attach great importance to. At present, the problem of excessive heavy metals in the soil of agricultural land in my country has gradually become an environmental problem that threatens the ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/04C12N11/087C12N1/20B09C1/10C02F11/02C12R1/41C02F101/20
CPCC12N11/14C12N11/04C12N11/087C12N1/20B09C1/10C02F11/02C02F2101/20
Inventor 张维谊丰东升邓波朱春燕王敏宋宇迎周雨璊尚禹李瑞红
Owner 上海市农产品质量安全中心
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