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Application of lactobacillus plantarum in preparation of lactein, lactic acid and phenyllactic acid

A technology of Lactobacillus plantarum and lactobacillus, applied in the field of fermentation engineering, can solve the problems of high cost, long time consumption, poor accuracy, etc., and achieve the effect of increasing the diameter of inhibition

Active Publication Date: 2022-02-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these methods have the disadvantages of poor accuracy, long time-consuming and high cost, and are not suitable for large-scale industrial application. Therefore, it is necessary to invent a fast, accurate and low-cost lactobacillus quantitative method

Method used

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  • Application of lactobacillus plantarum in preparation of lactein, lactic acid and phenyllactic acid
  • Application of lactobacillus plantarum in preparation of lactein, lactic acid and phenyllactic acid
  • Application of lactobacillus plantarum in preparation of lactein, lactic acid and phenyllactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Screening of lactic acid bacteria for efficient production of probiotic metabolites

[0046] 14 strains of lactic acid bacteria preserved in the laboratory (Lactobacillus plantarum DY1, Lactobacillus paracasei DY2, Lactobacillus paracasei DY3, Lactobacillus rhamnosus DY4, Pediococcus lactis DY5, Lactobacillus plantarum DY6, Lactobacillus plantarum DY7, Rhamnos Gram-positive strains with good antibacterial effect were screened from Lactobacillus sugarus DY8, Lactobacillus plantarum DY9, Lactobacillus plantarum DY10, Enterococcus faecalis DY11, Lactobacillus casei DY12, Lactobacillus casei DY13, Lactobacillus rhamnosus DY14) .

[0047] Specific steps are as follows:

[0048] 1. Prepare MRS medium:

[0049] 10.0g peptone, 8.0g beef extract, 4.0g yeast powder, 20.0g glucose, 2.0g dipotassium hydrogen phosphate, 2.0g triammonium citrate, 5.0g sodium acetate, 0.58g magnesium sulfate heptahydrate, 0.25g manganese sulfate tetrahydrate , Tween 80 1mL, distilled wat...

Embodiment 2

[0063] Embodiment 2: Lactobacillus plantarum DY6 fermentation medium optimization

[0064] Specific steps are as follows:

[0065] (1) In order to obtain the best fermentation medium composition of antibacterial performance, the present embodiment proposes a kind of improved MRS medium formula of lactic acid bacteria after optimization, from glucose (10,20,30,40g / L), yeast powder ( 0, 5, 10, 15g / L), peptone (0, 5, 10, 15g / L), and ammonium citrate (0, 1, 2, 4g / L), a single factor experiment was designed;

[0066] Specific steps are as follows:

[0067] Inoculate a single colony of Lactobacillus plantarum DY6 in MRS liquid medium, and culture it statically for 16-18 hours at 37°C to prepare seed liquid, and transfer the seed liquid to 50mL MRS medium at a ratio of 2% (v / v) Plantarum cultured at 37°C for 24 hours to obtain Lactobacillus plantarum liquid, 8000r·min -1 After centrifuging for 15min, adopt the Oxford cup method antibacterial test in the specific embodiment to dete...

Embodiment 3

[0082] Example 3: Determination of prebiotic metabolites of Lactobacillus plantarum DY6

[0083] Specific steps are as follows:

[0084] (1) According to the result of embodiment 2 step (1), the following medium is selected as the improved MRS medium:

[0085] Peptone 5g, Yeast powder 15g, Glucose 40g, Dipotassium hydrogen phosphate 2g, Triammonium citrate 2g, Sodium acetate 5g, Magnesium sulfate heptahydrate 0.58g, Manganese sulfate tetrahydrate 0.25g, Tween 80 1mL, Distilled water 1L, 115℃ Sterilize for 30min.

[0086] (2) Inoculate a single colony of Lactobacillus plantarum DY6 into the improved MRS medium prepared in step (1), and culture it statically at 37°C for 16-18 hours to obtain a seed liquid, which is prepared by 2% The inoculum size of (v / v) was transferred to 50 mL of the above-mentioned improved MRS medium, and cultured at 37° C. for 24 hours to obtain Lactobacillus plantarum fermentation broth;

[0087] The prepared fermented liquid was heated at 8000r·min ...

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Abstract

The invention discloses application of lactobacillus plantarum in preparation of lactein, lactic acid and phenyllactic acid, and belongs to the technical field of fermentation engineering. According to the method, lactobacillus plantarum CCTCC No: M 2017138 with high antibacterial activity is adopted, and the lactic acid content in metabolite obtained by fermenting lactic acid bacteria for 48 hours reaches 127.3 g / L and the lactein content reaches 46.08 mg / L by improving the formula of an MRS culture medium and optimizing the fermentation conditions of a fermentation tank. The lactobacillus plantarum CCTCC No: M 2017138 is applied to be combined with an improved MRS culture medium, and fermentation conditions of a fermentation tank are optimized, so that the content of probiotic metabolites such as lactein, lactic acid and phenyllactic acid in lactobacillus metabolites is remarkably increased, the antibacterial performance is enhanced, and intestinal health is facilitated.

Description

technical field [0001] The invention relates to the application of plant lactobacillus in preparing lactobacillus, lactic acid and phenyllactic acid, and belongs to the technical field of fermentation engineering. Background technique [0002] Lactic acid bacteria (LAB) are Gram-positive, non-spore-free, inactive or inactive bacteria that ferment sugar and produce a large amount of lactic acid. Lactic acid bacteria are rich in species and widely distributed. They can not only endow food with high nutritional value and good flavor, but also have disease resistance and antibacterial ability, regulate the balance of intestinal flora, and then regulate other organ functions and alleviate diseases such as metabolic syndrome. It has a promoting effect and is widely used in livestock and poultry breeding, food industry, pharmaceutical manufacturing and other fields. The probiotic metabolites produced by lactic acid bacteria include organic acids, fatty acids, bacteriocins, protein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12P7/40C12P21/00C12N1/20C12N1/38C12R1/25
CPCC12P7/56C12P7/40C12P21/00C12N1/20C12N1/38Y02A50/30
Inventor 邓禹周胜虎刘颖颖贾以泽毛银李国辉赵运英
Owner JIANGNAN UNIV