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Sennoside extraction method

An extraction method and technology of sennoside, applied in the field of sennoside extraction, can solve the problems of poor stability of sennoside, difficult and high-efficiency extraction, easy decomposition and transformation, etc., and achieve convenient separation and purification, low cost, and easy to scale up production. Effect

Pending Publication Date: 2022-02-11
YANGZHOU AIDEA BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The steps are cumbersome, and the last step of purification process uses highly toxic organic solvents such as acetone and methanol
In addition, sennoside has poor stability, and it is easy to decompose and transform into monoanthrone components when exposed to heat and light, and it is difficult to efficiently extract this component by conventional extraction and macroporous adsorption chromatography.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: (1) Medicinal material extraction: get 100g senna medicinal material (wherein sennoside A+sennoside B content is 1.25%) add 8 times (g / mL) 0.1% sodium bicarbonate solution stirring extraction 60min after filtering , to obtain the extract (extract sampling test), add 6mol / L HCl to adjust the pH to 3.0, remove impurities, filter and leave the supernatant, add sodium chloride to adjust the electric rate to 20mS / cm, add 5mol / L NaOH to adjust the pH to 3.5, obtain the sample solution (acid adjustment sample solution sampling detection);

[0026] (2) Sample loading: 300 mL of A451 (ZG A451 type, Hangzhou Zhengguang Resin Co., Ltd.) macroporous weakly basic anion-exchange chromatography column is pre-installed, at a linear velocity of 60 cm / h, equilibrated with an equilibrium solution of 5 times the column volume, Equilibrium liquid is 0.02mol / L glycine buffer solution, which contains 0.2mol / L NaCl, pH is 3.5, conductivity is 23mS / cm, and then the sample solution...

Embodiment 2

[0030] Embodiment 2: (1) medicinal material extraction: get 100g senna medicinal material (wherein sennoside A+sennoside B content is 1.23%) add 10 times (g / mL) 0.25% sodium bicarbonate solution and stir to extract 90min after filtering , to obtain the extract (sampling detection), add 6mol / L HCl to adjust the pH to 4.5, filter to leave the supernatant, add sodium chloride to adjust the conductivity to 15mS / cm, add 5mol / LNaOH to adjust the pH to 7.0, and obtain the sample solution ( Acid adjustment sample solution sampling test);

[0031](2) Sample loading: 300mL macroporous weakly basic anion exchange chromatography column of A351 type (ZG A351 type, Hangzhou Zhengguang Resin Co., Ltd.) is pre-installed, and the linear velocity is 60cm / h. Liquid is 0.02mol / L phosphate buffered saline, wherein contains 0.15mol / L NaCl, and pH is 7.0, and conductivity is 18mS / cm, then the sample solution in step (1) is loaded sample layer with linear velocity 60cm / h Analysis column.

[0032] (...

Embodiment 3

[0035] Embodiment 3: (1) medicinal material extraction: get 1000g senna medicinal material (wherein sennoside A+sennoside B content is 1.15%), add 10 times (g / mL) 0.25% sodium bicarbonate solution and stir to extract 120min after filtration , to obtain the extract (extract sample detection), add 6mol / L HCl to adjust the pH to 5.5, filter to leave the supernatant, add sodium chloride to adjust the conductivity to 7mS / cm, add 5mol / L NaOH to adjust the pH to 10.0, and obtain the above Sample solution (acid adjustment sample solution sampling test);

[0036] (2) Sample loading: 1500 ml of A313 type (ZG A313 type, Hangzhou Zhengguang Resin Co., Ltd.) macroporous weakly basic anion exchange chromatography column is pre-installed. It is 0.2mol / L sodium bicarbonate buffer solution, which contains 0.1mol / L NaCl, pH is 10.0, and conductivity is 11mS / cm, and then the sample solution in step (1) is loaded into the sample layer at a linear velocity of 60cm / h Analysis column.

[0037] (3)...

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Abstract

The invention discloses a sennoside extraction method, and the method comprises the following steps: (1) primary extraction of a medicinal material: adjusting the pH value of an extracting solution obtained by soaking folium sennae to 2.5-5.5, filtering and reserving a supernatant, adding sodium chloride into the obtained supernatant to adjust the conductivity, and adjusting the pH value of the supernatant to 4.0-10.0 to obtain a sample loading solution, wherein the conductivity of the loading liquid is 2-5mS / cm lower than that of the equilibrium liquid; (2) sample loading: balancing an ion exchange chromatographic column by using equilibrium liquid in advance, and loading the sample loading liquid in the step (1) to the chromatographic column; (3) elution: after sample loading is finished, flushing with equilibrium liquid by 3-5 times of column volume, and then flushing the column with eluent by 3-5 times of column volume; (4) drying of the eluent obtained in the step (3) under reduced pressure to obtain a powdery extract of the sennoside A / B compound. According to the method, the sennoside is separated and purified by adopting ion exchange chromatography, the sennoside A and the sennoside B can be separated from the folium sennae extracting solution, and the recovery rate is high.

Description

Technical field [0001] The invention relates to the technical field of plant extraction, and in particular to a sennoside extraction method. Background technique [0002] Folium sennae is one of the commonly used laxative drugs. It is the dried leaf of the leguminous plant Cassiaangustifolia Vahl or C.acutifolia Delile. The main active ingredients in senna are anthraquinone derivatives. Research in recent years has found that among the anthraquinone derivatives, the dianthrone derivatives sennosides A, B, C, and D have stronger purgative effects and irritation than other anthraquinone-containing laxatives, and can be taken orally The drug has little toxicity and is commonly used clinically for acute constipation. Dianthrones are composed of two molecules of anthrones C-C connected to each other. Among them, sennosides A and B, and sennosides C and D are stereoisomers of each other. Sennoside has inhibitory effects on Streptococcus pneumoniae, Klebsiella pneumoniae and Sta...

Claims

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Application Information

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IPC IPC(8): C07H15/244C07H1/08
CPCC07H15/244C07H1/08
Inventor 吴佳佳曾扬尹池玉梅黄媛王建强袁玉王军傅和亮
Owner YANGZHOU AIDEA BIOTECH
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