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Method for extracting type II collagen from chicken sternal cartilage and product thereof

A collagen and cartilage technology, applied in the field of collagen extraction technology, can solve the problems of uneven molecular weight distribution and low absorption efficiency, etc.

Pending Publication Date: 2022-02-15
海南盛美诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Type II collagen products currently on the market have uneven molecular weight distribution and low absorption efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for extracting type II collagen by using chicken breast cartilage and its products, the preparation method of the type II collagen and its products mainly includes the following preparation steps:

[0032] (1) Degreasing: Immerse the fresh chicken breast cartilage with bone and flesh removed in a sodium hydroxide solution with a mass fraction of 0.3%, at 20°C, 30kHz ultrasonic vibration for 30min, then wash with pure water until neutral, at -10°C temperature, 5Pa pressure Dried for 6 hours under the oven to obtain chicken breast cartilage after degreasing;

[0033] (2) Grinding: the degreased chicken breast cartilage is first pulverized at an ambient temperature of -10°C until the particles are smaller than 9mm, and then placed in a grinder, and ground at an ambient temperature of -10°C until the particles are smaller than 1mm to obtain Chicken breast cartilage powder;

[0034](3) Acid hydrolysis: Mix chicken breast cartilage powder and 0.3% hydrochloric acid ...

Embodiment 2

[0048] A method for extracting type II collagen by using chicken breast cartilage and its products, the preparation method of the type II collagen and its products mainly includes the following preparation steps:

[0049] (1) Degreasing: Immerse the fresh chicken breast cartilage with bone and meat removed in a sodium hydroxide solution with a mass fraction of 0.4%, at 25°C, 35kHz ultrasonic vibration for 25min, then wash with pure water until neutral, at -8°C temperature, 8Pa pressure Drying for 5 hours under the hood makes the chicken breast cartilage after degreasing;

[0050] (2) Grinding: the degreased chicken breast cartilage is pulverized at -8°C ambient temperature until the particles are less than 9mm, and then placed in a pulverizer, and ground at -8°C ambient temperature until the particles are smaller than 1mm. Chicken breast cartilage powder;

[0051] (3) Acid hydrolysis: Mix chicken breast cartilage powder and 0.4% hydrochloric acid solution evenly at a mass rat...

Embodiment 3

[0065] A method for extracting type II collagen by using chicken breast cartilage and its products, the preparation method of the type II collagen and its products mainly includes the following preparation steps:

[0066] (1) Degreasing: Immerse the fresh chicken breast cartilage with bone and meat removed in a sodium hydroxide solution with a mass fraction of 0.5%, at 30°C, 40kHz ultrasonic vibration for 20min, then wash with pure water until neutral, at -5°C temperature, 10Pa pressure Dried for 4 hours under the hood to obtain degreased chicken breast cartilage;

[0067] (2) Grinding: the degreased chicken breast cartilage is pulverized at an ambient temperature of -5°C until the particles are smaller than 9mm, and then placed in a pulverizer, and ground at an ambient temperature of -5°C until the particles are smaller than 1mm. Chicken breast cartilage powder;

[0068] (3) Acid hydrolysis: Mix chicken breast cartilage powder and 0.5% hydrochloric acid solution evenly at a ...

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PUM

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Abstract

The invention discloses a method for extracting type II collagen from chicken sternal cartilage and a product thereof, and relates to an extraction process of collagen. When the II-type collagen is extracted, the II-type collagen is prepared by taking chicken sternal cartilage as a raw material through degreasing, grinding, acidolysis, enzymolysis, electrophoresis dialysis, secondary grinding and sterilization, and then the II-type collagen is subjected to carrier membrane preparation, secondary electrophoresis and vacuum storage to prepare the product. The collagen dressing film product prepared by the invention has a good wound healing effect.

Description

technical field [0001] The invention relates to a collagen extraction process, in particular to a method for extracting type II collagen by using chicken breast cartilage and a product thereof. Background technique [0002] Chicken breast cartilage is a piece of white cartilage on the chicken bone near the chicken breast. It has a crisp taste. Among them, type II collagen is the most important organic component in the cartilage matrix. It has a dense fiber structure and is a characteristic protein of cartilage tissue. Chicken breast cartilage is cheap and easy to obtain, and has high content of type II collagen, so it is a good source of type II collagen extraction. Chicken breast cartilage can be used to extract type II collagen, which can be made into various nutritional products and skin care products, so as to realize the appreciation and diversified utilization of resources. [0003] The molecular weight of type II collagen is about 300,000 daltons, and the digestion a...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/26C07K1/14C07K1/12A61L15/32A61L15/18A61L15/42
CPCC12P21/06C07K14/78A61L15/325A61L15/18A61L15/425C08L89/00
Inventor 陈忆宾杨维
Owner 海南盛美诺生物技术有限公司
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