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Nano fluorescence sensor as well as preparation method and application thereof

A nano-fluorescence and sensor technology, applied in the field of fluorescent sensing materials, can solve problems such as single property, photobleaching, solid-state quenching of fluorescent probe materials, etc., achieve good photostability, enhance detection limit, and avoid photobleaching Effect

Pending Publication Date: 2022-03-18
NEW MATERIAL INST OF SHANDONG ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In order to overcome the above-mentioned technical problems existing in the prior art, the present invention provides a nano-fluorescence sensor and its preparation method and application, to overcome the solid-state quenching and photobleaching of fluorescent probe materials, and pure MSNs due to their simple structure , single in nature, not fully equipped with practical application value and other technical defects

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  • Nano fluorescence sensor as well as preparation method and application thereof
  • Nano fluorescence sensor as well as preparation method and application thereof
  • Nano fluorescence sensor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0055] The preparation of organic fluorescent molecule DHBM comprises the following steps:

[0056] (1) Sodium bisulfite (0.97g, 9.3mmol) and 4-(diethylamino) salicylaldehyde (1.79g, 9.3mmol) were reacted in absolute ethanol (50mL) at room temperature for 12h;

[0057] (2) Dissolve 3,4-diaminobenzoic acid (1.41g, 9.3mmol) in 50mL DMF, then add it to the reaction mixture obtained in step (1), reflux at 80°C for 12h, cool the reaction bottle to room temperature, solvent in Evaporate under reduced pressure; Then add deionized water (100mL), a large amount of precipitate occurs;

[0058] (3) The precipitate obtained in step (2) was collected by filtration, and dried in vacuum for 2 days to obtain the yellow product DHBM with a yield of 2.39 g, 75%. 1 H NMR (400MHz, DMSO-d6) δ (ppm) 12.77 (s, 3H, PhCOOH, PhOH, C-NH), 8.11 (s, 1H, PhH), 7.85 (s, 1H, PhH), 7.79 (d, 1H,PhH),7.61(d,1H,PhH),6.39(d,1H,PhH),6.21(s,1H,PhH),3.41(m,4H,CH 2 ),1.15(t,6H,CH 3 ).ESI+HR-MS calcd for DMBM[M+H]...

Embodiment 2

[0061] NH 2 -The preparation of MSN comprises the following steps:

[0062] (1) Dissolve CTAB (200mg) in 100mL of distilled water, add NaOH aqueous solution (0.7mL, 2M); after stirring the resulting mixed solution at 80°C for 30min, add 1mL of ethyl orthosilicate under vigorous stirring (800rpm) , stirred for 4h, cooled to room temperature and filtered to collect the precipitate;

[0063] (2) The precipitate obtained in step (1) was fully washed 3 times with deionized water and ethanol, and dried in vacuum at room temperature for 2 days; then the product was placed in a muffle furnace at 500°C for 5 hours to remove the template and obtain the product MSN;

[0064] (3) Put 200mg of MSN and 2mL of (3-aminopropyl)triethoxysilane into 10mL of toluene, stir at 30°C for 12h under nitrogen protection, and collect by centrifugation to obtain the product NH 2 -MSN, after which methanol washed 3 times and vacuum dried for 2 days.

[0065] For the obtained product NH 2 -Observe the m...

Embodiment 3

[0067] The preparation of DHBM-MSN includes the following steps:

[0068] DHBM (0.02g, 0.09mmol), N-hydroxysuccinimide (0.01g, 0.09mmol) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (0.02g, 0.09mmol) was suspended in 5mL DMF, stirred at room temperature for 4h; then, 100mg of the NH obtained in Example 2 was added 2 -MSN, stirred at 30°C for 12h. The product was separated by centrifugation, washed three times with methanol, and vacuum-dried to obtain a product powder, which was designated as DHBM-MSN.

[0069] The porosity of the prepared DHBM-MSN was measured, and the result was 0.82cm 3 / g.

[0070] In the prepared DHBM-MSN, the proportion of DHBM on the surface and pores of mesoporous silica was detected, and the result was 55%.

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Abstract

The invention discloses a nano fluorescent sensor as well as a preparation method and application thereof, and belongs to the technical field of fluorescent sensing materials. According to the material, organic fluorescent molecules synthesized by 3, 4-diaminobenzoic acid and 4-(diethylamino) salicylaldehyde through a simple synthesis process are grafted into mesoporous silicon dioxide in a post-grafting manner, and the organic-inorganic hybrid mesoporous material not only shows ultra-sensitive metal ion detection performance, but also can be used for detecting metal ions. Meanwhile, due to the good biocompatibility, the fluorescence sensor can be practically applied to fluorescence labeling and cell imaging in cells, and the fluorescence sensor has huge application potential in the fields of in-vivo sensing labeling and biomedicine.

Description

technical field [0001] The invention belongs to the technical field of fluorescence sensing materials, and in particular relates to a nanometer fluorescence sensor and its preparation method and application. Background technique [0002] Selective detection of heavy metal ions has received increasing attention due to their important impact on health and sustainable environments. Cu 2+ As an important transition metal element, it is widely used in industrial and agricultural production, and it is also an essential trace element for animals, plants and human body. However, excessive intake of Cu 2+ It will have adverse effects on the life activities of the human body, so Cu 2+ Also considered one of the most widespread and dangerous pollutants. Therefore, to develop a simple and effective method for accurate monitoring of Cu 2+ method is imminent. In recent years, fluorescent probes have become one of the most important detection tools due to their advantages of convenie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D235/18C09K11/02C09K11/06G01N21/64
CPCC07D235/18C09K11/06C09K11/025G01N21/6428C09K2211/1007C09K2211/1044
Inventor 高萌邢晨晨徐利洁刘雨虹李勇李东玮孙佩佩王猛
Owner NEW MATERIAL INST OF SHANDONG ACADEMY OF SCI
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