Method for promoting microorganisms to induce carbonate precipitation by using attapulgite clay
A carbonate precipitation, attapulgite clay technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high economic cost of calcium chloride, restrictions on popularization and application, and achieve no secondary pollution. , good application prospect, simple extraction effect
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[0055] (3) Preparation of liquid medium: the present invention adopts nutrient broth medium (NB): peptone 10g, sodium chloride 5g, beef extract 3g, attapulgite clay extract 1000ml, pH is adjusted to 6.8 ± 0.2, 121 ℃ high temperature and high pressure sterilization for 20min. The physical and chemical properties and water-soluble calcium and magnesium ion contents of the above-mentioned silver attapulgite clay (B-ATP) are shown in Table 1.
[0056] (4) Expansion of bacterial strains: Inoculate the preserved bacterial strains in 50ml nutrient broth (NB) medium in an ultra-clean bench, the inoculum size is 1%-2%, and cultivate them in a constant temperature oscillator at 30°C and 150rpm , regularly detect its absorbance value OD 600 (Optical density600), until its absorbance value OD 600 Around 1.
[0057] (5) Generation and identification of microorganism-induced calcite precipitation: inoculate the enriched bacteria solution of (4) in the culture medium of (3) in an ultra-cl...
Embodiment 1
[0067] Four kinds of attapulgite clay water-soluble extracts provide material basis for MICP to remove heavy metal arsenic (As) in aqueous solution;
[0068] The first step is to use the water-soluble extract of attapulgite clay (ATP) to prepare a liquid medium: weigh 35g of silver attapulgite clay, add 350ml of distilled water at a solid-to-liquid ratio of 1:10, shake for 2 hours (200rpm, 25°C), Centrifuge (4000rpm, 10min), filter, and take 300ml extract for later use.
[0069] In the second step, the blank group (CK) and the control group are prepared Na with an As concentration of 20 mg / L with distilled water. 3 AsO 4 solution and nutrient broth mixture, the experimental group used the attapulgite extract to prepare Na 3 AsO 4 solution and nutrient broth mix. Use 50ml Erlenmeyer flasks to do three parallel experiments. Sterilize under high temperature and high pressure at 121°C for 20 minutes, lower to room temperature, inoculate 1ml of the enriched bacterial solution ...
Embodiment 2
[0073] Attapulgite clay provides a material basis for MICP in the soil and solidifies the heavy metal arsenic (As);
[0074] The first step, the enrichment of the bacterial solution: according to the amount required for the experiment, the LF strain was amplified and cultured with NB liquid medium (the inoculum size was 1%-5%); the soil sample was pretreated, and the collected soil sample was naturally Air-dried, ground, passed through a 2mm sieve, and set aside.
[0075] The second step is to cultivate the enriched bacteria solution for 18-20 hours, the control group: soil + urea + LF bacteria + CaCl 2 , wherein, the bacterial solution was added according to the solid-liquid ratio of 7:3, the amount of urea added was 12%, and the CaCl 2 The amount of addition is 60mM, and LF is added in the form of bacterial solution. Experimental group: soil + urea + LF bacteria + attapulgite clay, the amount of urea and bacteria liquid added is the same as that of the control group, the a...
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