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Gynecological gel and preparation method thereof

A gel and gynecological technology, applied in the direction of medical formula, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the side effects of atrophic vaginitis, etc., to increase resistance, good water solubility and the effect of adhesion

Pending Publication Date: 2022-04-19
尹文君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this application is to provide a gynecological gel, which aims to solve the problem of side effects of existing hormone therapy for atrophic vaginitis

Method used

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  • Gynecological gel and preparation method thereof
  • Gynecological gel and preparation method thereof
  • Gynecological gel and preparation method thereof

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preparation example Construction

[0054] The present application also provides a preparation method of the above-mentioned gynecological gel, comprising:

[0055] Adding the exosome solution and Cnidium Fructus Extract to the gel solution for the first stage of stirring;

[0056] Add berberine and matrine to the mixed solution, carry out the second-stage stirring, and obtain the gynecological gel after the stirring is completed.

[0057] Preferably, the exosomes are extracted from stem cells treated with hypoxia, and the oxygen concentration of the hypoxia treatment is 5%-10%.

[0058] Preferably, the oxygen concentration of the hypoxic treatment is 5%.

[0059] Hypoxia treatment stimulates stem cells to secrete more exosomes, and can make stem cells secrete more cytokines, increasing the number of cytokines contained in exosomes, so that exosomes have better therapeutic effects.

[0060] Preferably, the extraction method of exosomes includes: any one of differential high-speed centrifugation, density gradie...

Embodiment 1

[0066] 1.1 Preparation of exosomes

[0067] 1.11 Isolation and culture of human amniotic mesenchymal stem cells

[0068] Take the amniotic membrane after cesarean section, wash the amniotic membrane with DPBS containing 1% double antibody (DPBS solution contains penicillin 100U / ml, streptomycin 0.1mg / ml), and use tissue scissors and tweezers to remove coagulated blood clots and excess organize. Cut the amnion into pieces with tissue scissors, add 0.5% trypsin and shake and digest at 37°C for 1 hour, then filter the digested tissue through a 200-mesh sieve, wash thoroughly with the above-mentioned DPBS containing 1% double antibody, and then place Tissue fragments were collected and digested with 1 mg / ml collagenase 1 at 37°C for 1 h with shaking. Filter the digested tissue through a 200-mesh sieve, take the filtrate, and centrifuge at 1500 rpm / min for 5 min to collect the cell pellet. Resuspend the cell pellet in DMEM / F12 medium and culture at 37°C, 5% CO 2 In the cell cul...

Embodiment 2

[0079] The difference from Example 1 is that the exosomes of the gynecological gel in Example 2 come from normal cultured human amniotic mesenchymal stem cells that have not undergone hypoxic treatment, and the rest of the steps are the same as in Example 1, and will not be repeated here.

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Abstract

The invention provides gynecological gel and a preparation method thereof.The gynecological gel comprises exosome, gel, sophocarpidine, berberine and a fructus cnidii extracting solution, the exosome is obtained through cell secretion, contains multiple cell factors such as EGF, can play a composite role in cell repairing, can repair damaged vaginal epithelial cells, and is free of toxic and side effects. Matrine, berberine and fructus cnidii are antibacterial and anti-inflammatory traditional Chinese medicines. The gel does not have pungent smell, is viscous, has good water solubility and adhesion, can be well adsorbed into the female vagina, has a neutral pH value, slowly reduces the viscosity of the gel in an acidic vaginal environment, gradually releases the coated exosome and traditional Chinese medicine components, and covers damaged vaginal tissues. The vagina inflammation can be effectively controlled and treated through the traditional Chinese medicine components, the exosome can act on damaged vagina epithelium to promote repair of epithelial cells, and the gel is attached to a diseased region, so that the effect of protecting a wound surface is achieved.

Description

technical field [0001] The present application relates to the technical field of biomedicine, in particular to a gynecological gel and a preparation method thereof. Background technique [0002] Atrophic vaginitis is one of the diseases that menopausal women are prone to suffer from. It is common in women after natural menopause and ovarian castration. Increased vaginal pH. The thinned epithelium has reduced resistance to vaginal flora, making it easy for pathogenic bacteria to invade the vagina and cause inflammation. Common pathogenic bacteria include: Streptococcus, Staphylococcus, Escherichia coli, etc. The main symptom is a small amount of vaginal bleeding , itching, difficulty urinating, or dyspareunia. Vaginal atrophy is characterized by pale mucosa, ecchymosis, and disappearance of folds. Microscopically, cells in the superficial and middle layers decrease or disappear to varying degrees. Small ulcers with acute inflammation and granulation tissue are distributed i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/234A61K9/06A61K47/32A61P15/02A61P15/00A61K35/28A61K31/4375
CPCA61K35/28A61K31/4375A61K36/234A61K9/06A61K9/0034A61K47/32A61P15/02A61P15/00A61K2300/00
Inventor 曾鹏辉李熙阳浣溢帆周英杰欧阳贵熊智缘
Owner 尹文君
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