Reusable multi-index microfluidic detection system based on coding microspheres and use method

A technology of encoding microspheres and detection systems, applied in fluid controllers, chemical instruments and methods, measuring devices, etc., can solve the problem of solid-phase chip detection speed limited single-layer probe molecules, and an integrated real-time detection system is yet to be developed , solid-phase chip waste of raw materials and other issues, to achieve the effect of convenient and fast multi-index detection, improved reaction speed and flexibility, and short detection time

Pending Publication Date: 2022-04-19
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although quantum dot fluorescence-encoded microspheres / solid-phase chips are an extremely convenient multi-indicator detection method, their actual detection application faces the following difficulties: 1. The laboratory operation is relatively cumbersome, and a practical integrated real-time detection system is not yet available. To be developed; 2. The one-time use of solid-phase chips will waste a lot of raw materials, and it is imminent to develop chips that can repeat experiments; 3. The detection speed of solid-phase chips is limited by the single-layer probe molecules marked on the substrate. How to improve the detection speed? Speed ​​is a problem; Fourth, how to integrate the inlet and outlet, mixer, detector, etc. on the microfluidic chip still needs to be explored

Method used

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  • Reusable multi-index microfluidic detection system based on coding microspheres and use method
  • Reusable multi-index microfluidic detection system based on coding microspheres and use method
  • Reusable multi-index microfluidic detection system based on coding microspheres and use method

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Embodiment 1

[0058] Before the present invention is used, the oligonucleotide probe is modified on the surface of the quantum dot fluorescent coding microsphere of 100nm, and on the Fe 3 o 4 - The polymer-coated 300nm magnetic nanosphere surface is modified with streptavidin, and the nucleic acid sequence to be tested is PCRed with biotinylated primers to form a large number of biotinylated ssDNA molecules. Make a mixer chip and a detector chip for the number of nucleic acid sequences to be detected, and fix them on the chip platform.

[0059] Describe the process of nucleic acid detection by the present invention, the steps are as follows:

[0060] 1. Inject the modified streptavidin magnetic microsphere solution and the biotinylated ssDNA sequence solution from the two inlets of the mixer respectively. Turn on the switch of the ultrasonic transducer, let the two pass through the mixer slowly, and fully mix the reaction.

[0061] 2. When the fluid in the mixer flows through and fills t...

Embodiment 2

[0067] Carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) immunodetection method based on the present invention

[0068] Before the use of the present invention, prepare the red quantum dot fluorescent microsphere solution marked by anti-CEA Ab1, prepare the green quantum dot fluorescent microsphere solution marked by anti-AFP Ab1, and mix the above two solutions in equal concentrations; prepare anti- CEEAb2 labeled magnetic nanosphere solution, prepare anti-AFP Ab2 labeled magnetic nanosphere solution, make a mixer chip and two detector chips, and fix them on the chip stage.

[0069] 1. Inject two kinds of magnetic microsphere solutions with different labels from the outlets of the two detectors 10. When the detector microchannel is filled with magnetic nanospheres, start the electromagnet to adsorb the magnetic balls on the bottom surface of the channel, and continue to reverse direction. Inject the buffer solution to wash away the solvent of the microsphere solution...

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Abstract

The reusable multi-index microfluidic detection system comprises a microfluidic mixer, an ultrasonic transducer and a microfluidic detector which are arranged on a chip table, an electromagnet is arranged on the lower portion of the microfluidic detector, an optical detection unit is arranged on the upper portion of the microfluidic detector, and the optical detection unit is arranged on the chip table. The micro-fluidic mixer and the micro-fluidic detector are formed by bonding a PDMS (Polydimethylsiloxane) micro-channel and a glass substrate. Multi-index nucleic acid sequence / immunodetection can be carried out, tedious operation and large-scale detection equipment in a laboratory are replaced, the required sample amount is small, the detection time is short, magnetic beads marked by probe molecules are utilized, tedious steps of chip internal modification are omitted, the reaction speed and flexibility are improved, liquid phase reaction in a micro-channel is achieved, repeatability is good, precision is high, and the method is suitable for large-scale industrial production. Quantitative detection can be realized, and the chip can be reused.

Description

technical field [0001] The invention relates to the field of microfluidic detection, in particular to a reusable multi-index microfluidic detection system based on coded microspheres and a method for using it. Background technique [0002] Microfluidic chips are composed of micro- / nano-level microchannels and various surrounding devices, and are a new POCT (point of care testing) detection method in recent years. Microfluidic technology refers to the precise path control of the fluid flowing inside it by designing various microstructures and microchannels, so as to achieve various control purposes. Microfluidic chips can be used for cell sorting, purification, and enrichment, and can also be used as micro-reaction chambers to provide reaction vessels for biological detection. Antigen-antibody immune detection, nucleic acid detection, etc. can all be carried out in microfluidic chips. Compared with traditional large-scale instruments, microfluidic chips have the advantages ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N33/543G01N33/58G01N33/53G01N33/574G01N33/68
CPCB01L3/5027B01L3/50273B01L3/502715B01L3/502738G01N33/54326G01N33/54346G01N33/588G01N33/5308G01N33/57473G01N33/68G01N2333/471B01L2200/0636B01L2200/10B01L2300/02B01L2300/0861B01L2400/0439B01L2300/0654B01L2400/0406
Inventor 崔大祥唐浩刘关牛嘉琦
Owner SHANGHAI JIAO TONG UNIV
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