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Biological agent for treating arthritis as well as preparation method and application of biological agent

A technology of biological preparations and arthritis, applied in the field of biomedicine, can solve the problems of immune rejection of patients who are not easy to prepare, donor trauma, and difficulty in obtaining, etc., and achieve the effect of alleviating the progression of osteoarthritis, promoting cartilage regeneration, and rich sources

Pending Publication Date: 2022-04-22
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, stem cells and other biological agents currently used in the treatment of knee osteoarthritis are not easy to obtain, will cause trauma to the donor when taking materials, are difficult to prepare, and cause immune rejection in patients.

Method used

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  • Biological agent for treating arthritis as well as preparation method and application of biological agent
  • Biological agent for treating arthritis as well as preparation method and application of biological agent
  • Biological agent for treating arthritis as well as preparation method and application of biological agent

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Part I Extraction and Identification of Human Urine-derived Stem Cells

[0038] The extraction process of human urine-derived stem cells is as follows: figure 1 As shown, specifically, take 50mL-250mL of human clean middle urine, put it in a 50mL centrifuge tube, centrifuge at 400g for 10min, discard the upper urine in a biological safety cabinet, add 40mL containing 1% double antibody (Gibco) PBS, mixed with the bottom urine, 400g, centrifuged for 5min. Repeat the step of washing the urine with PBS twice. Discard the supernatant, add 5mL of urine-derived stem cell (hUSCs) medium to resuspend the cells at the bottom of the centrifuge tube, then inhale the cell suspension into a T25 culture flask, and put it in a 37°C 5% CO2 incubator for observation.

[0039] The well-grown third-generation USCs were taken, and the expression of cell surface antigens (including CD24, CD29, CD44, CD73, CD90, CD105, CD133, and CD34) was detected by flow cytometry. The chondrocytes were ...

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Abstract

The invention relates to a biological agent for treating arthritis as well as a preparation method and application of the biological agent. The biological agent is prepared from urinary stem cell exosome rich in miR40-5p. The urinary stem cell exosome suspension rich in miR40-5p is used for joint cavity injection treatment of a patient suffering from knee osteoarthritis, and meanwhile, the mesenchymal stem cells and the miR40-5p are combined to relieve osteoarthritis progress and promote cartilage regeneration, so that the biological hidden danger during stem cell injection treatment and the poor targeting property of miR40-5p injection treatment are avoided; the injection type biological preparation for treating knee osteoarthritis is a novel injection type biological preparation for treating knee osteoarthritis, which is rich in innovation and clinical conversion value.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a biological preparation for treating arthritis, a preparation method and application thereof. Background technique [0002] In the existing guidelines and expert consensus, osteoarthritis adopts a stepwise treatment plan of basic treatment, drug treatment, restorative treatment, and reconstructive treatment. Among them, non-steroidal drugs (NSAIDs) with anti-inflammatory and analgesic effects were used for drug treatment. Secondly, intra-articular injection of adrenal corticosteroids or sodium chondroitin sulfate, hyaluronic acid and other protective agents for articular cartilage treatment. In addition, as a therapeutic drug for joint degenerative diseases, a p21-activated kinase (PAK) antagonist (Japanese Patent Application Publication No. 2007-537134 ), which is a signal cascade system antagonist, or an antisense polynucleotide or ribozyme A pharmaceutical c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/22A61P19/02A61P29/00C12N5/071C12N15/113C12N15/867
CPCA61K35/22A61P19/02A61P29/00C12N5/0602C12N15/113C12N15/86C12N2740/15043
Inventor 沈彬曾羿刘渊吴元刚李明阳李帅
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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