Fe3O4/MoO3-x-GOD-PVP nano-enzyme as well as preparation method and application thereof
A moo3-x-god-pvp, moo3-x-god technology, applied in the field of biomedical nanomaterials, can solve the problems of drug resistance of tumor cells, limited therapeutic effect, drug leakage, etc., and achieve excellent dual catalytic performance, Improve the effect, enhance the effect of efficiency
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Embodiment 1
[0044] Step 1, 1.25g FeCl·H 2 O was dissolved in 7.75 mL of water. Under vigorous stirring, 6.25 mL of ammonia water (25%) was added to the above aqueous solution, followed by stirring in air for 10 minutes. The above mixture was added to a 50 mL stainless steel autoclave lined with polytetrafluoroethylene. Then, 5 mL of polyethyleneimine aqueous solution (108 mg / mL) was further added to the autoclave. After stirring, the reaction vessel was placed at 134° C. for 3 hours, and then the mixture was cooled to room temperature. The black precipitate was collected by magnetic separation and purified 5 times with water to remove excess reactants and by-products. Finally, the Fe 3 o 4 The nanoparticles were vacuum dried at 37°C for 24 h for further use.
[0045] Step 2, mix 2mmol molybdenum powder with 24mL ethanol. With vigorous stirring, 3 mL of H 2 o 2 (30%) was added to the above mixture. After 1 h, the mixture was transferred to a 50 mL Teflon-lined stainless steel aut...
Embodiment 2
[0050] Step 1, 1.25g FeCl·H 2 O was dissolved in 7.75 mL of water. Under vigorous stirring, 6.25 mL of ammonia water (25%) was added to the above aqueous solution, followed by stirring in air for 10 minutes. The above mixture was added to a 50 mL stainless steel autoclave lined with polytetrafluoroethylene. Then, 5 mL of polyethyleneimine aqueous solution (108 mg / mL) was further added to the autoclave. After stirring, the reaction vessel was placed at 134° C. for 3 hours, and then the mixture was cooled to room temperature. The black precipitate was collected by magnetic separation and purified 5 times with water to remove excess reactants and by-products. Finally, the Fe 3 o 4 The nanoparticles were vacuum dried at 37°C for 24 h for further use.
[0051] Step 2, mix 2mmol molybdenum powder with 24mL ethanol. With vigorous stirring, 3 mL of H 2 o 2 (30%) was added to the above mixture. After 1 h, the mixture was transferred to a 50 mL Teflon-lined stainless steel aut...
Embodiment 3
[0056] Get the Fe prepared by appropriate embodiment 1 3 o 4 / MoO 3-x -GOD-PVP nanozyme was pressed into KBr tablets, and the infrared absorption was tested using an infrared spectrometer. Such as figure 2 As shown, Fe 3 o 4 / MoO 3-x - The infrared spectra of GOD-PVP nanozyme at 1001 and 853 cm -1 The nearby peaks are attributed to Mo=O stretching vibrations and Mo-O-Mo bonds. 1123cm -1 Nearby absorption, which is attributed to the C–O stretching vibration of glucose oxidase. 1283cm -1 is the stretching vibration of the polyvinylpyrrolidone N-C bond, which proves that the Fe 3 o 4 / MoO 3-x - Composition of GOD-PVP nanozymes.
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