Immobilization method for improving biological hydrogen synthesis of hydrogen-producing bacteria and application

A bacterial and biological technology is applied in the field of preparation of novel coupled nanomaterial immobilizers, which can solve the problems of low biological hydrogen production efficiency, and achieve the effects of promoting the utilization of reducing sugars, being easy to control, and having mild reaction conditions.

Active Publication Date: 2022-04-29
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on raw peach gum as the raw material, the immobilization coupling nanoparticle addition is expected to develop a low-cost, high-efficiency hydrogen-producing bacteria immobilization method to promote biohydrogen synthesis, and to solve the key technical problem of low biohydrogen production efficiency

Method used

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  • Immobilization method for improving biological hydrogen synthesis of hydrogen-producing bacteria and application
  • Immobilization method for improving biological hydrogen synthesis of hydrogen-producing bacteria and application
  • Immobilization method for improving biological hydrogen synthesis of hydrogen-producing bacteria and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]Embodiment 1: the preparation of raw peach gum green synthetic nano-magnetite:

[0036] Weigh 1g of peach gum powder and add it to 100mL of distilled water, place it in a water bath at 80°C for 8 hours at a constant temperature, make the peach gum powder swell completely, and form a peach gum liquid; further adjust the pH value to 10-11, and place it on a constant temperature magnetic stirrer , after heating and stirring at 85°C for 3h, then cooling down to 50°C, adding a certain volume of 0.5mol / L Fe to the peach gum solution 3+ and 0.75mol / L Fe 2+ solution, so that Fe in the mixture 3+ : Fe 2+ =2:1, placed on a constant temperature magnetic stirrer, and stirred continuously at 60°C for 4h. The Fe 3+ The salt is ferric chloride; the Fe 2+ The salt is ferrous sulfate. The reaction solution was cooled to room temperature, and the newly prepared 2mol / L NaOH solution was slowly added while stirring until the precipitates all turned black, and then the reaction was con...

Embodiment 2

[0037] Embodiment 2: the preparation of raw peach gum immobilized gel beads:

[0038] The raw peach gum collected from peach trees is air-dried after removing bark and other sundries, and then crushed into peach gum powder by a traditional Chinese medicine grinder for later use. Weigh 4g of peach gum powder and add it to 100mL of distilled water, and place it in a water bath at 80°C for 8 hours at a constant temperature to make the peach gum powder swell completely and form a peach gum solution; after cooling the obtained peach gum solution properly, add 2g of sodium alginate to After mixing, place in a high-pressure steam sterilizer to sterilize at 121°C for 20 minutes. Simultaneously pick the activated Klebsiella hydrogen-producing single bacterium colony in the liquid medium (recipe: D-xylose 10g, glucose 10g, beef extract 5g, peptone 10g, NaCl 5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 1g, pH 7.5, water 1000mL) for overnight culture, further centrifuged at low speed (4000r / mi...

Embodiment 3

[0039] Example 3: Green synthesis of nano-magnetite to regulate hydrogen production and reducing sugar utilization of rice straw hydrolyzate fermented by Klebsiella:

[0040] The nano-magnetite synthesized from raw peach gum was added to the rice straw hydrolyzate fermentation hydrogen production system according to the concentration of 0, 10, 20, 30, 40, and 50 mg / L, and the 0 mg / L treatment was the control treatment (CK). Accurately weigh nano-magnetite according to different treatments, add to fermentation medium (recipe: rice straw hydrolyzate 1000mL, beef extract 5g, peptone 10g, NaCl 5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, sugar concentration 50g / L, pH 8.0), adopt the method of sufficient stirring combined with ultrasonic treatment to effectively disperse the nanoparticles in the fermentation medium, after sterilizing and cooling, inoculate Klebsiella according to 10% inoculum Bacteria seed liquid, regularly use the sodium hydroxide solution method to detect the hyd...

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Abstract

The invention discloses an immobilization method for improving biological hydrogen synthesis of hydrogen-producing bacteria and application, and relates to the technical field of bacterial agent immobilization. The immobilization method mainly comprises the following steps: synthesizing nano magnetite by taking original peach gum as a raw material; preparing the original peach gum into a solution, adding sodium alginate with the mass 0.5 times that of the original peach gum, uniformly mixing the solution with the nano magnetite synthesized by the peach gum with a certain concentration and a hydrogen-producing bacterium suspension, and dropwise adding the mixture into a CaCl2 solution to form immobilized gel beads. The immobilized gel bead is applied to a lignocellulose hydrolysate fermentation hydrogen production system, the viable count of the system can be increased, the accumulated hydrogen production after 120-hour fermentation is improved by about 60%, and the utilization rate of glucose and xylose in hydrolysate is remarkably improved. According to the invention, the nano magnetite prepared by taking the original peach gum as a raw material is used as an additive, and the original peach gum is used as a main immobilization carrier to prepare the high-efficiency immobilized microbial inoculum for the hydrogen-producing bacteria, so that the method is simple and convenient, the conditions are mild, the stability is good, the survival rate of the bacteria is high, and the hydrogen production promoting effect is obvious.

Description

technical field [0001] The present invention relates to a preparation method and application field of a novel coupling nanomaterial immobilizing agent, in particular to a preparation method and application of synthesizing nano-magnetite from raw peach gum and coupling peach gum immobilizing agent, especially to the The effect of the new immobilizing agent in regulating the biohydrogen synthesis of hydrogen-producing bacteria is applied in the field of efficient synthesis and regulation of biohydrogen energy. Background technique [0002] Immobilized hydrogen-producing bacterial cell technology has become an important technology to improve the hydrogen-producing ability of bacteria because it can increase system stability, expand the activity range of hydrogen-producing bacteria, and improve the system's ability to tolerate pH changes. Immobilized cell methods can be divided into embedding method, system interception method, adsorption method and cross-linking method accordin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/14C12N11/04C12N11/02C12P3/00C01G49/08B82Y40/00C12R1/22C12R1/01
CPCC12N11/10C12N11/14C12N11/04C12N11/02C12P3/00C01G49/08B82Y40/00
Inventor 张琴张永贵许思远曹娟娟赵沛叶景钱程
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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