Detection method of hypochlorite ions

A technology of hypochlorite and detection method, applied in the field of detection, can solve problems such as uneven distribution of probes, lack of internal reference, etc., achieve the effects of wide application range, reduce interference of irrelevant factors, and improve detection sensitivity and accuracy

Active Publication Date: 2022-05-06
HEFEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Current fluorescence spectroscopic methods for the detection of hypochlorite ions lack an effective internal reference, often display single-wavelength emission, and are easily interfered by instrument parameters, photobleaching, changes in the microenvironment around the probe, and uneven distribution of the probe

Method used

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  • Detection method of hypochlorite ions
  • Detection method of hypochlorite ions
  • Detection method of hypochlorite ions

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: a kind of detection method of hypochlorite ion

[0036] A method for detecting hypochlorite ions comprises the following technical steps.

[0037] (1) Determination of curcumin standard curve

[0038] The curcumin powder used was purchased from Aladdin Reagent (Shanghai) Co., Ltd., with a purity greater than 98%.

[0039] Step 1: Weigh the curcumin powder and place it in a 1.5mL PE tube, add dimethyl sulfoxide solution to prepare a curcumin solution with a concentration of 1mmol / L, ready for use.

[0040] Step 2: Take six 1.5mL PE tubes and number them 1-6. Pipette 1.0 μL, 1.6 μL, 2.2 μL, 2.8 μL, 3.4 μL, and 4.0 μL of curcumin solutions with a concentration of 1 mmol / L into tubes 1-6 in sequence, and then add 199.0 μL, 198.4 μL, 197.8 μL, 197.2 μL, 196.6 μL, 196.0 μL dimethyl sulfoxide-deionized water mixed solution (V 二甲基亚砜 / V 水 =1 / 4), mix thoroughly. Then pipette into the cuvette, measure the ultraviolet absorption A1, A2, A3, A4, A5, A6, do three ...

Embodiment 2

[0076] Embodiment 2: a kind of detection method of hypochlorite ion

[0077] The difference from Example 1 is that the "lake water" was taken from Hefei Emerald Lake on January 10, 2022, and the water temperature measured during the experiment was 25°C.

[0078] Take a certain volume of lake water and filter it with a 0.22 μm pore size filter to remove impurities and set it aside. Pipette 7.0μmol / L[Ru(bpy) 3 ] 2+ Put @CCM-NPs in a 1.5mL PE tube, then add lake water to make up to 200mL. After reacting for 154.0 seconds at 25°C, move it to a cuvette, measure the fluorescence emission spectrum of the sample under 365nm excitation with a fluorescence spectrophotometer, and record the fluorescence emission spectrum intensity F at 503nm and 603nm 503nm , F 603nm . Measured F 603nm / F 503nm =0.74087, substitute it into the above regression equation F 603nm / F 503nm =0.18166C ClO - +0.70179, the concentration of hypochlorite is 0.2151 μmol / L.

Embodiment 3

[0079] Embodiment 3: A kind of detection method of hypochlorite ion

[0080] A detection method for hypochlorite ions, comprising the following steps:

[0081] Step 1: measure curcumin standard curve;

[0082] Step 2: Synthetic curcumin liposome;

[0083] Step 3: Assemble the dual emission ratiometric fluorescent probe [Ru(bpy) 3 ] 2+ @CCM-NPs;

[0084] Step 4: Construct the regression equation of hypochlorite ion fluorescence detection;

[0085] Step 5: Pretreatment of samples containing hypochlorite ions;

[0086] The sample to be tested is tap water, liquid food or environmental water; tap water and liquid food are tested directly; the environmental water is filtered with a filter paper or membrane with a pore size of 0.22 μm before testing, and the filtrate is collected for testing;

[0087] Step 6: Sample detection and hypochlorite ion concentration calculation;

[0088] First pipette the dual emission ratiometric fluorescent probe [Ru(bpy) 3 ] 2+ Put the @CCM-NP...

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Abstract

The invention relates to a hypochlorite ion detection method, which comprises: 1, determining a curcumin standard curve; step 2, synthesizing curcumin liposome; step 3, assembling a dual-emission-ratio fluorescent probe [Ru (bpy) 3] < 2 + > (at) CCM-NPs; 4, constructing a hypochlorite ion fluorescence detection regression equation; 5, pretreating a sample containing hypochlorite ions; and step 6, sample detection and hypochlorite ion concentration calculation. The invention designs and assembles the ratio-type fluorescent probe, and the ratio-type fluorescent probe can effectively eliminate or reduce interference of irrelevant factors of analytes through self-calibration of two emission wavebands of the ratio-type fluorescent probe, so that the detection sensitivity and accuracy are improved.

Description

technical field [0001] The invention relates to a method for detecting hypochlorite ions, which belongs to the technical field of detection. Background technique [0002] Hypochlorous acid is a highly reactive oxygen species that widely exists in living organisms. It can react with biomolecules such as proteins, nucleic acids, and fatty acids, and plays an important role in the human immune system. However, excessive hypochlorous acid in the human body can trigger oxidative stress and cellular damage in the body, eventually leading to a series of diseases such as lung injury, atherosclerosis, arthritis, neurodegeneration, and cancer. In daily life, hypochlorite solution is also widely used as an antiseptic, bleach and disinfectant. Excessive use of hypochlorite solution will damage the respiratory system of the human body, make the semicarbazide in the food exceed the standard, and react with organic matter in the water to form carcinogens such as carbon tetrachloride, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/6419Y02A20/20
Inventor 沈益忠朱春蕾叶应旺魏云龙陈欢欢
Owner HEFEI UNIV OF TECH
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