Preparation method and application of brevibacillus laterosporus and spore antibacterial peptide-chitosan-gelatin composite preservative film

Abstract

The invention discloses a strain of brevibacillus laterosporus capable of producing antibacterial peptide, the name of the brevibacillus laterosporus is B07-2, the classification name of the brevibacillus laterosporus is brevibacillus laterosporus, the preservation number of the brevibacillus laterosporus is GDMCC No.62281, the preservation date of the brevibacillus laterosporus is March 7, 2022, the preservation unit of the brevibacillus laterosporus is Guangdong Province Microbial Culture Collection Center, and the preservation address of the brevibacillus laterosporus is No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The brevibacillus laterosporus B07-2 is a strain of probiotics, and the brevibacillus laterosporus B07-2 and the bacteriostatic active substances secreted by the brevibacillus laterosporus B07-2 have high thermal stability and acid-base tolerance. The spore antibacterial peptide-chitosan-gelatin composite preservative film prepared by the preparation method disclosed by the invention can effectively inhibit harmful microorganisms of a food source, and the used materials are environment-friendly, safe, degradable and edible, and do not cause harm to the environment and a human body.

Description

technical field [0001] The invention belongs to the fields of food preservation and biotechnology, in particular to a preparation method and application of an antimicrobial peptide-producing biocontrol bacterium Brevibacillus lateralosporus, and a spore antimicrobial peptide-chitosan-gelatin composite preservative film. Background technique [0002] At present, the traditional plastic wrap has the following problems: [0003] 1. Traditional polymeric plastic wrap is non-degradable. [0004] 2. The structural components of traditional polymeric plastic wrap may cause harm to human health. [0005] 3. Traditional plastic wrap has single function. [0006] The main reasons for the above problems are: the conventional cling film is a plastic packaging product, which is generally made of ethylene as the masterbatch through polymerization reaction. The cling film can be divided into three categories: the first is polyethylene, referred to as PE; the second is It is polyvinyl ch...

AI Technical Summary

Problems solved by technology

[0003] 1. Traditional polymeric plastic wrap is non-degradable

[0004] 2. The structural components of traditional polymeric plastic wrap may cause harm to human health

[0005] 3. The function of traditional plastic wrap is single

[0008] 1. Aiming at the problem that the above-mentioned traditional plastic wrap is non-degradable and poses hidden dangers to human health

In the prior art ("A Preparation Method of Novel Edible Fruit and Vegetable Preservative Film", patent application number ZL201811036917.1), the soybean polysaccharide in the bean dregs is extracted and stirred with chitosan, sodium alginate, and glycerin to make a preservative film , the preservative film formula is green, safe, economical and environmentally friendly, but its function is single, it does not have antibacterial effect, and it cannot upgrade the fresh-keeping effect

[0009] 2. In view of the lack of green and safe antistaling agents, the existing technology ("A Food Preservative", patent application number CN201710278924.1), uses natamycin and acidic substances to form the base material of food antistaling agents, natamycin As a natural food preservative of microbial origin, natamycin can meet the requirements of food antisepsis and preservation and people's requirements for food safety, but natamycin, as an antibiotic bacteriostatic agent, is likely to cause pathogenic bacteria resistance, thereby reducing the antibacterial and fresh-keeping effects even disappear

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