Lipopeptide compound bacteriostatic agent suitable for trichoderma and penicillium on bone cultural relics as well as preparation method and application of lipopeptide compound bacteriostatic agent

Abstract

The invention relates to the field of microorganism and cultural relic protection, and particularly discloses a lipopeptide compound bacteriostatic agent suitable for trichoderma and penicillium on bone cultural relics as well as a preparation method and application of the lipopeptide compound bacteriostatic agent. The provided lipopeptide compound bacteriostatic agent is prepared from safe and harmless lipopeptide as a main bacteriostatic component and two bacteriostatic drugs, namely ketoconazole and chlorhexidine gluconate, the lipopeptide is extracted after being fermented by bacillus amyloliquefaciens M73 researched and developed by a key microbe laboratory of Sichuan University, and the lipopeptide compound bacteriostatic agent is simple to prepare, clear in component, low in cost, low in cost and suitable for industrial production. The lipopeptide compound bacteriostatic agent has a good bacteriostatic effect on isolated bacteria on the three-star-heap ancient ivory, and meanwhile, the lipopeptide compound bacteriostatic agent does not generate adverse effects such as color change and material damage when applied to three-star-heap ancient ivory fragments.

Description

technical field [0001] The invention relates to the fields of biotechnology bacteriostasis and cultural relic protection, in particular to a lipopeptide compound bacteriostatic agent for Trichoderma and Penicillium on bone cultural relics and a preparation method thereof, as well as the bacteriostasis of fungi on Sanxingdui ancient ivory application. Background technique [0002] Cultural relics are relics or relics with historical, artistic and scientific value left by human beings in social activities, which are precious historical and cultural heritage of human beings. For example, Sanxingdui ancient ivory has extremely high historical value, cultural value and scientific value, and provides new clues for the exploration of the roots of Chinese civilization. [0003] Although a large area of ​​cultural relics has been unearthed, the preservation of cultural relics is a recognized world problem. Once the cultural relics are exposed to the air, it is extremely easy to bre...

AI Technical Summary

Problems solved by technology

[0004] The existing research reports on antibacterial agents for cultural relics are as follows: 1) He Qiuju's "Screening and Evaluation of Antifungal Agents for Several Archaeologically Unearthed Lacquer and Woodware" [J], which mainly focus on chemical drug antibacterial agents currently on the market. , compared the antibacterial effects of four different antifungal agents, and found that 0.5g / mL dichlorooctylisothiazolinone had a significant antifungal effect; but the chemical drugs used in this paper have greater corrosion to cultural relics

2) Li Tianxiao's "Research on the Cognition and Prevention of Typical Microbial Diseases in Feilaifeng Formation [D], measured the antibacterial effects of three inorganic bacteriostatic agents and six organic antibacterial agents on Feilaifeng Formation, and determined the commercial antibacterial agent AW -600 (the active ingredient is octhilone) has the best antibacterial effect, but its antibacterial agent is single, and the antibacterial effect needs to be further improved

Although these chemical antibacterial agents can play an effective bactericidal effect, they are corrosive or toxic to a certain extent, and may cause adverse damage to the cultural relics themselves.

5) CN109645029A[P] discloses the preparation and application of an antifungal agent for cultural relics. In this patent, the fermentation liquid containing lipopeptide active ingredients produced by Bacillus subtilis is used as an antifungal agent to target Penicillium and Aspergillus niger has a good antibacterial effect, but in addition to lipopeptide active ingredients, the fermentation broth also contains cells and a large number of other metabolites, which may cause adverse effects on cultural relics; at the same time, the antibacterial agent is only effective against Penicillium and Aspergillus niger to the antibacterial effect, and these antibacterial agents mainly inhibit the growth of fungi, and on cultural relics, in addition to fungi, there are also a large number of bacterial growth

[0008] The prior art discloses the use of Bacillus subtilis as an antibacterial agent for cultural relics, but it not only has poor bacteriostatic effect, but also has no compound, and the bacteriostatic effect cannot reach the effect of effective and long-term protection of cultural relics, and has a certain effect on cultural relics. damage

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