Liposome drug carrier combined with blood cell membrane as well as preparation method and application of liposome drug carrier

A liposome and cell membrane technology, applied in liposome delivery, pharmaceutical formulations, drug combinations, etc., can solve the problems of red blood cell inactivation, inability to have long circulation time, and drug loading limitation of red blood cells, and achieve good activity and function. Good application prospects and the effect of prolonging the half-life of drugs

Pending Publication Date: 2022-05-13
BLOOD TRASFUSION INST CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method of fusion with red blood cells is basically to extract blood shadows of red blood cells first, and then to load drugs. The red blood cells lose their activity and cannot have a long circulation time.
The above existing problems limit...

Method used

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  • Liposome drug carrier combined with blood cell membrane as well as preparation method and application of liposome drug carrier
  • Liposome drug carrier combined with blood cell membrane as well as preparation method and application of liposome drug carrier
  • Liposome drug carrier combined with blood cell membrane as well as preparation method and application of liposome drug carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1, the fusion of drug-loaded liposome and erythrocyte membrane prepares drug carrier preparation

[0062] 1. Preparation of paclitaxel-loaded liposomes

[0063] Dissolve 59.5mg of dipalmitoylphosphatidylcholine (DPPC) and 3.5mg of cholesterol (CHO) in 3mL of chloroform, and add 1.8mg of paclitaxel (PTX) to dissolve, and then carry out thin film vacuum at 45-50°C Rotary steam for 60 min, then add 5 mL of phosphate buffer saline (PBS) with pH=7.4, and hydrate at 50-55°C for 30 min. The hydrated product passes successively through polycarbonate membranes with pore diameters of 0.4 μm, 0.2 μm and 0.1 μm for 3-5 times each to obtain a paclitaxel-loaded liposome solution. The encapsulation efficiency of paclitaxel-loaded liposomes was as high as 91.14%±1.84% as detected by HPLC. Paclitaxel-loaded liposomes were named PTX-Liposome (DPPC).

[0064] 2. Preparation of dexamethasone-loaded liposomes

[0065] Dissolve 59.5mg of dipalmitoylphosphatidylcholine (DPPC) a...

Embodiment 2

[0070] Embodiment 2, the fusion of drug-loaded liposome and erythrocyte membrane prepares drug carrier preparation

[0071] 1. Preparation of paclitaxel-loaded liposomes

[0072] The preparation method of paclitaxel-loaded liposome (PTX-Liposome) is the same as that in Example 1.

[0073] 2. Preparation of dexamethasone-loaded liposomes

[0074] The preparation method of dexamethasone-loaded liposome (DEX-Liposome) is the same as that in Example 1.

[0075] 3. Fusion of paclitaxel-loaded liposomes with erythrocyte membrane

[0076] The paclitaxel-loaded liposome solution obtained in step 1 was resuspended in PBS buffer (pH=7.4). Mix 120 μL of paclitaxel-loaded liposome solution with a concentration of 2 μmol and human red blood cells (3×10 8 RBC) were incubated (37°C, 4h), centrifuged (3500g, 10min) three times, the erythrocytes without fused liposomes were separated from the erythrocytes fused with liposomes, and the supernatant was removed to obtain erythrocytes fused wi...

Embodiment 3

[0079] Embodiment 3, preparation of drug carrier preparation by fusion of drug-loaded liposome and erythrocyte membrane

[0080] 1. Preparation of paclitaxel-loaded liposomes

[0081] The preparation method of paclitaxel-loaded liposome (PTX-Liposome) is the same as that in Example 1.

[0082] 2. Preparation of dexamethasone-loaded liposomes

[0083] The preparation method of dexamethasone-loaded liposome (DEX-Liposome) is the same as that in Example 1.

[0084] 3. Fusion of paclitaxel-loaded liposomes with erythrocyte membrane

[0085] The paclitaxel-loaded liposome solution obtained in step 1 was resuspended in PBS buffer (pH=7.4). Mix 180 μL of 3 μmol paclitaxel-loaded liposome solution with human red blood cells (3×10 8 RBC) were incubated (37°C, 4h), centrifuged (3500g, 10min) three times, the erythrocytes without fused liposomes were separated from the erythrocytes fused with liposomes, and the supernatant was removed to obtain erythrocytes fused with liposomes The ...

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Abstract

The invention provides a liposome drug carrier combined with a blood cell membrane as well as a preparation method and application of the liposome drug carrier, and belongs to the technical field of biological materials. The liposome drug carrier disclosed by the invention is obtained by mixing liposome and blood cells; the lipidosome is adhered to the surface of a blood cell membrane or the lipidosome is fused with the blood cell membrane. The liposome drug carrier combined with the blood cell membrane is prepared, the liposome drug carrier can carry different kinds of drugs, the drug encapsulation efficiency and the drug loading capacity are high, and the delivery efficiency of the drugs can be effectively improved. Meanwhile, according to the drug carrier disclosed by the invention, the liposome is combined with cell membranes of blood cells with biological activity, and the combined blood cells can still keep complete morphology and good activity and functions, so that the half-life period of the drug can be effectively prolonged, and the elimination of an autoimmune system can be better avoided; more effective drug sustained release and clinical treatment effects are achieved with a lower drug dosage, and good application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a liposome drug carrier combined with blood cell membranes, a preparation method and application thereof. Background technique [0002] Due to the untargeted release of broad-spectrum hydrophobic chemotherapeutic drugs, conventional chemotherapy using such drugs usually causes severe toxic side effects (hair loss, gastrointestinal reactions, cardiotoxicity, and nervous system damage), and lacks tumor targeting, thus lead to unsatisfactory treatment effects. In recent years, new particle drug delivery systems such as drug-loaded nanoparticles, liposomes and microspheres have attracted more and more attention because of their significant advantages such as high loading efficiency and controllable release behavior. At the same time, as an exogenous substance, it is easy to be cleared by the human immune system after administration, and its bioavailability is low. ...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/46A61K45/00A61K31/337A61P35/00
CPCA61K9/1271A61K47/46A61K45/00A61K31/337A61P35/00
Inventor 曹晔朱珂慧徐莹璨王红钟锐刘嘉馨
Owner BLOOD TRASFUSION INST CHINESE ACAD OF MEDICAL SCI
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