Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hybrid capture kit for genome target region sequencing, capture method and application

A target area, hybridization capture technology, applied in the field of hybridization capture kits, can solve the problems of low capture efficiency, difficult to meet, high price, etc., and achieve the effect of improving capture efficiency, reducing experimental cost, and good capture efficiency and uniformity

Pending Publication Date: 2022-05-13
上海英基生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many commercial kits using liquid phase hybridization capture technology, but they are mainly foreign industry giants, and the price is high
Domestic products generally have the problem of low capture efficiency, which reduces the effective data volume of the sample and virtually increases the cost of reagents for a single sample, making it difficult to meet the current needs of targeted sequencing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hybrid capture kit for genome target region sequencing, capture method and application
  • Hybrid capture kit for genome target region sequencing, capture method and application
  • Hybrid capture kit for genome target region sequencing, capture method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]The purpose of this example is to compare the performance difference between the kit and method of the present invention and several commercially available products of the same kind in hybrid capture. Among similar products, domestic 1: Hybridization Capture Kit (P10006, Homgen): Domestic 2: BOKE Capture Kit (TC003116, BOKE), two domestic brands. Abroad: xGen Hybridization and Wash Kit (1080584, IDT), a foreign brand, the test of similar products is carried out with reference to their respective instructions.

[0032] The operation steps of the kit of the present invention mainly include mixing and blocking the prepared library with Human Cot DNA and Blocker, hybridization between the sample and the probe after blocking, preparation of the elution buffer, preparation of streptavidin magnetic beads, magnetic bead capture and Washing and Post-capture PCR. details as follows:

[0033] 1 The prepared library is mixed with Cot DNA and Blocker and concentrated

[0034] 1.1 ...

Embodiment 2

[0120] This embodiment is to test the hybridization capture effect of betaine (Beta) and tetramethylammonium chloride (TMAC) in hybridization buffer with different ratios. The experimental operation process refers to Example 1, and will not be listed in detail here. The tested ratio is as follows:

[0121] test Beta TMAC Basic Hybridization Buffer Test1 2M 1.5M 2x SSPE, 5x Denhandt’s, 2mM EDTA, 0.02% SDS, 10% dextran sulfate Test2 2M 1M 2x SSPE, 5x Denhandt’s, 2mM EDTA, 0.02% SDS, 10% dextran sulfate Test3 2M 0.5M 2x SSPE, 5x Denhandt’s, 2mM EDTA, 0.02% SDS, 10% dextran sulfate Test4 1.5M 1.5M 2x SSPE, 5x Denhandt’s, 2mM EDTA, 0.02% SDS, 10% dextran sulfate Test5 1.5M 1M 2x SSPE, 5x Denhandt’s, 2mM EDTA, 0.02% SDS, 10% dextran sulfate Test6 1.5M 0.5M 2x SSPE, 5x Denhandt’s, 2mM EDTA, 0.02% SDS, 10% dextran sulfate Test7 1M 1.5M 2x SSPE, 5x Denhandt’s, 2mM EDTA, 0.02% SDS, 10% dextran sulfate Test8...

Embodiment 3

[0126] The following implementation example is to test the amount of hybridization buffer enhancer in the hybridization system. The experimental operation process refers to Example 1, which will not be listed in detail here. The enhancer is selected from formamide and guanidine thiocyanate. The adjustment of system and magnetic bead capture system are as follows:

[0127] Hybrid system:

[0128] test Formamide Guanidine Thiocyanate (6M) hybridization buffer probe NF Water Test1 2ul 8.5ul 4ul 2.5ul Test2 2.7ul 8.5ul 4ul 1.8ul Test3 3.5ul 8.5ul 4ul 1ul Test4 2ul 8.5ul 4ul 2.5ul Test5 2.7ul 8.5ul 4ul 1.8ul Test6 3.5ul 8.5ul 4ul 1ul Test7 2.7ul 1.8ul 8.5ul 4ul Test8 2.7ul 1ul 8.5ul 4ul 0.8ul Test9 2.7ul 0.5ul 8.5ul 4ul 1.3ul Test10 2.4ul 0.3ul 8.5ul 4ul 1.8ul Test11 2ul 0.7ul 8.5ul 4ul 1.8ul Test12 1.7ul 1ul 8.5ul 4ul 1.8ul

[0129] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biology, and particularly relates to a hybridization capture kit for genome target region sequencing, a capture method and application. The kit provided by the invention is suitable for genome libraries of various different sources, can effectively reduce the experiment cost and improve the capture efficiency, and is suitable for sequencing a genome target area. Experiments prove that compared with several commercially available similar kits, the kit provided by the invention has better capture efficiency, uniformity and other aspects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a hybrid capture kit, a capture method and an application for sequencing a genome target region. Background technique [0002] In recent years, with the rapid development of sequencing technology, high-throughput and high-accuracy next-generation sequencing technology (NGS) is being more and more widely used. High-throughput sequencing has greatly reduced the cost of sequencing. However, at present, the cost of whole-genome resequencing is still very high, and the huge amount of data obtained brings a lot of inconvenience to subsequent analysis, making it impossible for large-scale application. Targeted sequencing technology can enrich the genomic regions of interest for sequencing, greatly reducing the output of sequencing data, data analysis is simple and fast, and can take advantage of high-throughput sequencing technology more cost-effectively. Targeted sequencing t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6813C12Q1/6806C40B50/06C12Q1/6869
CPCC12Q1/6813C12Q1/6806C40B50/06C12Q1/6869C12Q2565/549C12Q2525/191C12Q2531/113C12Q2523/308C12Q2535/122
Inventor 冯延叶孙大坤柴智刘会珍孙扬
Owner 上海英基生物科技有限公司
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com