Rare earth complex doped DNA (deoxyribonucleic acid) crystal for detecting medicine as well as preparation method and application thereof
A technology of complexes and DNA chains, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0036] DNA was dissolved in ultrapure water after centrifugation, and a growth plate was used as a vessel for crystal growth, and the crystals were grown by vapor diffusion.
[0037] Mix three small crystal DNA strands (H:I:J) in a ratio of 3:3:1, take the DNA solution in the well, add buffer (sodium cacodylate, MgCl) 2 , NaCl, HEPES (pH=6)), DNA solution:buffer=1:1, 1M NaCl solution was injected around the well, and the best crystal growth was obtained. Each well was sealed with scotch tape, heated in an oil bath and slowly lowered from 60°C to 22°C to anneal the tiles and incubated in the incubator for one day to obtain small crystals.
[0038] Mix the seven large crystal DNA strands (A~G) in the same proportion, take the DNA solution into the well, add buffer, DNA solution:buffer=7:1, when the DNA concentration is small, the crystals are larger and the number is smaller, such as figure 2 a, so the size of the crystal can be adjusted by controlling the concentration of the...
Embodiment 2
[0041] DNA was dissolved in ultrapure water after centrifugation, and a growth plate was used as a vessel for crystal growth, and the crystals were grown by vapor diffusion. Mix three small crystal DNA strands (H:I:J) in a ratio of 3:3:1, take the DNA solution in the well, add buffer (sodium cacodylate, MgCl) 2 , NaCl, HEPES (pH=6)), DNA solution:buffer=1:1, 1M NaCl solution was injected around the well, and the best crystal growth was obtained. Each well was sealed with scotch tape, heated in an oil bath and slowly lowered from 60°C to 22°C to anneal the tiles and incubated in the incubator for one day to obtain small crystals.
[0042]Mix the seven large crystal DNA strands (A~G) in the same proportion, take the DNA solution into the well, add buffer, DNA solution: buffer = 28:1, when the concentration of DNA solution is too large, the crystal nucleation is too fast, and the crystal It is too late to grow completely, and the obtained crystals are smaller and more numerous, ...
Embodiment 3
[0045] DNA was dissolved in ultrapure water after centrifugation, and a growth plate was used as a container for crystal growth, and the crystals were grown by vapor diffusion.
[0046] Step (1), mix three small crystal DNA strands (H:I:J) in a ratio of 3:3:1, take the DNA solution in the well, add buffer (sodium cacodylate, MgCl) 2 , NaCl, HEPES (pH=6)), DNA solution:buffer=1:1, 1M NaCl solution was injected around the well, and the best crystal growth was obtained. Each well was sealed with scotch tape, heated in an oil bath and slowly lowered from 60°C to 22°C to anneal the tiles and incubated in the incubator for one day to obtain small crystals.
[0047] Step (2), mix the seven large crystal DNA strands (A~G) in the same proportion, take the DNA solution into the well, add buffer, DNA solution:buffer=14:1, inject NaCl solution around the well, transparent Tape to seal, heat slowly from 90°C to 22°C to anneal the tiles, and incubate in the incubator for three days to obta...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More