Potassium bacteria and potassium bacterial fertilizer
A technology of potassium-solubilizing bacteria and strains, which is applied to potassium fertilizers, bacteria, fertilization devices, etc., can solve problems such as differences in soluble potassium content in acidic purple soil, and achieve significant growth-promoting effects, increase potassium absorption, and alleviate the effects of potassium fertilizer deficiency.
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Embodiment 1
[0033] The potassium-solubilizing bacteria described in this example is a strain of the genus Stenotrophomonas, and the strain isolation, screening and identification processes are as follows.
[0034] Use the dilution plate method to screen purple soil potassium bacteria. The purple soil is collected from the acidic purple soil farmland area of Laobashan, Yucheng District, Ya'an City. The soil is collected at a depth of 0-30 cm, and the soil attached to the root system is gently shaken off in a sterile bag. taken back to the laboratory and stored in a refrigerator at 4°C. Weigh 10g of the soil sample into a conical flask with glass beads, add 90ml of sterile distilled water, shake at 180r / min, 25°C for 30min until fully shaken. Draw 1mL of the soil suspension into a sterile water test tube, dilute it 10 times with sterile water, pipette it evenly, and then make a 10-fold gradient dilution to prepare 10 times -4 、10 -5 、10 -6 Soil dilutions with three concentration gradie...
Embodiment 2
[0043] The potassium-dissolving bacterial fertilizer described in this embodiment includes the potassium-dissolving bacteria described in Example 1, and also includes a biochar carrier for loading the potassium-dissolving bacteria. In a further embodiment, potassium mineral powder is also included.
[0044] Preparation of biochar carrier
[0045] Corn straw charcoal, wheat straw charcoal and rice husk charcoal were prepared by oxygen-limited pyrolysis at 300℃, 500℃ and 700℃, respectively.
[0046] Rice husk charcoal scanning electron microscope figure 2 , figure 2 RHC means rice husk charcoal, RHC300 means rice husk charcoal prepared by oxygen-limited pyrolysis method at 300 °C, RHC500 means rice husk charcoal prepared by oxygen-limited cracking method at 500 °C, RHC700 means rice husk charcoal prepared by oxygen-limited cracking method Rice husk charcoal prepared by cracking method at 700°C.
[0047] Electron microscope scanning image of corn stalk charcoal Figure 4 ,...
Embodiment 3
[0063] This example is an application example of the potassium-dissolving bacterial fertilizer C-KSB and the potassium-dissolving bacterial fertilizer C-P-KSB described in Example 2. In this embodiment, ryegrass is taken as the test object, and the technical effects of the present invention are described.
[0064] 1.1 Preparation of bacterial suspension (KSB)
[0065] Inoculate the stenotrophomonas sp. strain preserved in glycerol into the potassium solution solid medium, and culture it upside down at 30°C for 5 days. Centrifuge the bacterial suspension at 10000r / min for 10 minutes, dump the excess culture solution, rinse with sterile distilled water several times and adjust the OD600 value to 1, and apply it to the soil at a mass ratio of 5%.
[0066] 1.2 Treatment of ryegrass seeds
[0067] Select annual ryegrass (Lolium perenne L. perenne L.) seeds with uniform grain size, weigh 1.5 times the required amount of seeds, add deionized water, stir and soak for 2 hours, remove...
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