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Quantitative detection method for mercury ions based on surface enhanced Raman scattering technology

A technology of surface-enhanced Raman and quantitative detection method, which is applied in the field of analysis and detection, can solve the problems of particle distribution volatility and inability to provide high enhancement factors, and achieve the effect of eliminating the interference of substances

Pending Publication Date: 2022-05-31
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the monitoring needs of the field environment, hand-held Raman is often used to monitor the environment, which requires relatively high requirements for the design of SERS-enhanced substrates. The above-mentioned SERS enhancement factor based on nanoparticles is low. In addition, because nanoparticles are mainly Using a liquid phase environment, the particle distribution fluctuates greatly, and the application is subject to certain limitations
However, the metal nanoarray structure based on nano-micro-micro-machining technology cannot provide a surface-enhanced Raman substrate with high enhancement factor and good reproducibility to solve the quantitative SERS detection of Hg ions due to the limitation of resolution.

Method used

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  • Quantitative detection method for mercury ions based on surface enhanced Raman scattering technology
  • Quantitative detection method for mercury ions based on surface enhanced Raman scattering technology
  • Quantitative detection method for mercury ions based on surface enhanced Raman scattering technology

Examples

Experimental program
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Embodiment 1

[0034] The preparation method of the Au nanofinger / DNA / Au nanofinger coupling structure includes the following steps:

[0035] Step 1: Au nano-finger ordered array structure is prepared by nano-imprinting, Au film deposition and reactive ion beam etching. The height of a single nano-finger is 300 nanometers, including 250 nanometers of polymer pillars and 50 nanometers of Au above, with a diameter of 50 nanometers. is 80 nanometers, and the distance between adjacent fingers is 120 nanometers, as shown in Figure 2(a) SEM photo;

[0036] Step 2: The experimental selection of thiolated single-stranded DNA sequence is 5'-HS-(CH2) 6-TTTTTTTTTTGGGGGGGAAAAAAAAAA, wherein T, G, A are base molecules of different structures, and tris-(2 formylethyl) phosphonium salt is used Acid (TCEP) reducing agent reduction treatment for use. The thiolated single-stranded DNA solution was thiol-activated and desalted, then all DNA samples were heated in hot water at 90°C for 20 minutes and then rapi...

Embodiment 2

[0041]High-selectivity and high-sensitivity quantitative detection of Hg ions based on Au nanofinger / DNA / Au nanofinger coupling structure, including the following steps:

[0042] Step 1: Titrate the solution containing mercury ions on the SERS-enhanced substrate of the DNA-modified Au nanofinger closed array structure, since the mercury ions and the intermediate base molecules in the adjacent 2 single-stranded DNAs form base molecules -Hg- The base molecule makes the single-stranded DNA lying on the surface of Au into a double-stranded DNA helix space structure perpendicular to the surface of Au, so that the terminal base molecules in the DNA are dissociated from the surface of Au, so as to stay away from the coupling formed in the gap The electromagnetic field is enhanced, and the intermediate base molecule is closer to the surface of Au, so its SERS signal is lower than that of the intermediate base molecule;

[0043] Step 2: Quantitative and highly selective detection of Hg...

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Abstract

The invention discloses a mercury ion quantitative detection method based on a surface enhanced Raman scattering technology, which comprises the following steps: firstly, obtaining a gold nano finger array structure through nanoimprinting, then modifying specific single-stranded DNA on the Au nano finger array structure through sulfur hydrogen bonds, collapsing the finger array structure in the volatilization process of a DNA solution, and finally titrating a mercury ion solution to detect the mercury ions. Single-stranded DNA lying on the surface of Au is changed into a double-stranded DNA spiral space structure perpendicular to the surface of Au, so that terminal base molecules in the DNA are far away from a coupling enhanced electromagnetic field formed in a gap, and an SERS (Surface Enhanced Raman Scattering) signal is reduced. According to the invention, interference-free high-selectivity and high-sensitivity detection of Hg ions in the mixed solution can be realized at the same time.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a mercury ion quantitative detection method based on surface-enhanced Raman scattering technology. Background technique [0002] Mercury(II) (Hg 2+ ) is a widespread pollutant in the water environment and may cause serious harm to human health. Mercury ions, especially methylmercury, can accumulate in the human body through the food chain and induce permanent damage to the kidneys, central nervous system, and digestive system, leading to a variety of chronic diseases (New England Journal of Medicine, 2003, 349(18) :1731-1737). Therefore, Hg 2+ The high-selectivity and high-sensitivity quantitative detection of selenium has been a long-term concern. A variety of Hg has been developed 2+ Detection methods, including organic dye probes, conjugated polymers, electrochemical anodic stripping voltammetry. These methods usually rely on coordination chemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N21/01
CPCG01N21/658G01N21/01G01N2021/0112
Inventor 刘凡新
Owner ZHEJIANG UNIV OF TECH
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