Eucommia staminate flower and eucommia staminate flower extract HPLC characteristic chromatogram construction method

Abstract

The invention discloses an establishment method of an HPLC (High Performance Liquid Chromatography) specific chromatogram of eucommia male flowers and extracts thereof and a fingerprint spectrum of the eucommia male flowers and the extracts thereof. The method comprises the following steps: step 1, preparing a test solution; 2, preparing a reference substance solution; step 3, chromatographic conditions: octadecylsilane chemically bonded silica is used as a filling agent; a mobile phase A is acetonitrile, a mobile phase B is a 0.1% phosphoric acid solution, and gradient elution is carried out; the detection wavelength is 206 nm; the column temperature is 10-30 DEG C; the flow rate is 1.0 ml.min <-1 >; the sample size is 10 microliters; and 4, determining to obtain the fingerprint spectrum of the eucommia male flowers. The invention also performs methodological verification on the establishment method of the fingerprint spectrum. The characteristic chromatogram of the eucommia male flower and the eucommia male flower extract measured by the method is high in precision, good in stability and good in repeatability, the quality of the eucommia male flower and the eucommia male flower extract can be effectively controlled, the quality uniformity and stability of the eucommia male flower, the eucommia male flower extract and related products are ensured, and the method is safe and effective.

Description

technical field [0001] The invention relates to a quality control method for traditional Chinese medicinal materials and extracts thereof, in particular to a method for constructing a characteristic map of male Eucommia ulmoides and its extracts by high performance liquid chromatography, and the characteristic map of male Eucommia ulmoides and its extracts obtained by the method . Background technique [0002] The male flower of Eucommia ulmoides is the flower of the male Eucommia ulmoides plant, and the male flower of Eucommia has high medicinal value since ancient times. Studies have shown that the natural active ingredients of Eucommia eucommia males have sedative, sedative and analgesic effects, and long-term use can significantly improve sleep. In addition, the essential collagen contained in the male Eucommia ulmoides has the effect of promoting muscle development and strengthening, and its active ingredient lignans have obvious anti-fatigue effects, which have a sign...

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Problems solved by technology

[0005] At present, there is no literature report to control the qu

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