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Composition containing platelet-rich plasma and preparation method thereof

A technology of platelet-rich plasma and composition, applied in the field of composition including platelet-rich plasma and its preparation, can solve problems such as joint pain, swelling and dysfunction, fast diffusion of cytokines, etc., to speed up and slow down the release speed Effect

Pending Publication Date: 2022-06-07
武汉冉谷医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, articular cartilage damage can also cause joint pain, swelling and dysfunction, seriously affecting the quality of life and health of patients
[0004] At present, the conventional treatment of osteoarthritis or articular cartilage injury is mainly based on physical therapy, traditional Chinese medicine treatment, drug therapy, and closed therapy, but the curative effect is not ideal, and the long-term curative effect is even worse. Sodium acid injection into the joint cavity, various cell regeneration therapies
However, the existing intra-articular injection of platelet-rich plasma has the problem of rapid diffusion of cytokines in platelet-rich plasma

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0020] The present specific embodiment provides a method for preparing a composition comprising platelet-rich plasma, comprising the following steps:

[0021] Dissolving chitosan in physiological saline to obtain a chitosan solution, then adding methacrylic anhydride to the chitosan solution, and stirring at 40-50° C. to obtain the methacrylic anhydride modified chitosan solution; the mass ratio of the methacrylic anhydride to the chitosan is 0.1-0.2:1; the mass concentration of chitosan in the chitosan solution is 2%-4%;

[0022] Mixing the methacrylic anhydride modified chitosan solution and the thrombin solution at 15-30° C. to obtain the first sub-composition; the volume ratio of the methacrylic anhydride modified chitosan solution and the thrombin solution is 1:1-1.2;

[0023] Mixing calcium chloride, graphene oxide, mesenchymal stem cells, collagen and platelet-rich plasma at 15-30° C. to obtain a second sub-composition; the concentration of the calcium chloride in the ...

Embodiment 1

[0034] The present embodiment proposes a composition comprising platelet-rich plasma, prepared by the following steps:

[0035] Dissolving chitosan in physiological saline to obtain a chitosan solution, then adding methacrylic anhydride to the chitosan solution, and stirring at 40° C. to obtain the methacrylic anhydride-modified chitosan solution; The mass ratio of the methacrylic anhydride to the chitosan is 0.1:1; the mass concentration of chitosan in the chitosan solution is 2%;

[0036] Mixing the methacrylic anhydride modified chitosan solution and the thrombin solution at 20° C. to obtain the first sub-composition; the volume ratio of the methacrylic anhydride modified chitosan solution to the thrombin solution is 1 :1;

[0037] The calcium chloride, graphene oxide, mesenchymal stem cells, collagen and platelet-rich plasma were mixed at 20°C to obtain a second sub-composition; the concentration of the calcium chloride in the second sub-composition was 30 mg / mL; the ma...

Embodiment 2

[0040] The present embodiment proposes a composition comprising platelet-rich plasma, prepared by the following steps:

[0041] Dissolving chitosan in physiological saline to obtain a chitosan solution, then adding methacrylic anhydride to the chitosan solution, and stirring at 50° C. to obtain the methacrylic anhydride-modified chitosan solution; The mass ratio of the methacrylic anhydride to the chitosan is 0.2:1; the mass concentration of chitosan in the chitosan solution is 3%;

[0042] The first sub-composition is obtained by mixing the methacrylic anhydride modified chitosan solution and the thrombin solution at 15°C; the volume ratio of the methacrylic anhydride modified chitosan solution and the thrombin solution is 1 :1.2;

[0043] A second sub-composition was obtained by mixing calcium chloride, graphene oxide, mesenchymal stem cells, collagen and platelet-rich plasma at 15°C; the concentration of the calcium chloride in the second sub-composition was 40 mg / mL; th...

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PUM

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Abstract

The invention discloses a composition containing platelet-rich plasma and a preparation method of the composition, and belongs to the technical field of biology. The preparation method of the composition comprising the platelet-rich plasma comprises the following steps: mixing a methacrylic anhydride modified chitosan solution and a thrombin solution at 15-30 DEG C to obtain a first sub-composition; mixing calcium chloride, graphene oxide, mesenchymal stem cells, collagen and platelet-rich plasma to obtain a second sub-composition; and mixing the first sub-composition and the second sub-composition to obtain the composition. The invention further provides a composition comprising the platelet-rich plasma, and the composition is prepared by the preparation method. The obtained composition shortens the time of changing the composition from a liquid state to a gel, and slows down the diffusion rate of cytokines at the same time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a composition comprising platelet-rich plasma and a preparation method thereof. Background technique [0002] Osteoarthritis is a degenerative disease, which is caused by aging, obesity, strain, trauma, congenital anomalies of the joints, joint deformities and many other factors. The disease is more common in middle-aged and elderly people, and it is more common in weight-bearing joints and joints with more activities (such as cervical spine, lumbar spine, knee joint, hip joint, etc.). Excessive weight bearing or overuse of these joints can promote degenerative changes. The clinical manifestations are slowly developing joint pain, tenderness, stiffness, joint swelling, limitation of motion and joint deformity. [0003] In addition, articular cartilage damage can also cause joint pain, swelling and dysfunction, which seriously affects the quality of life and physical health of patie...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61K35/19A61K35/28A61K9/06A61K47/02A61K47/04A61K47/36A61K47/42A61P19/02A61P19/08A61P29/00
CPCA61K9/06A61K35/16A61K35/28A61K35/19A61K47/36A61K47/42A61K47/02A61K9/0024A61P19/02A61P29/00A61P19/08A61K2300/00
Inventor 吴菲
Owner 武汉冉谷医疗有限公司
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