Paenibacillus polymyxa and application thereof in agriculture

A technology of Paenibacillus polymyxa and Bacillus, which is applied in the field of biological resource utilization, can solve problems such as poor control effect and limited types of biological agents, and achieve excellent control effect, strong adaptability, and the effect of promoting healthy development

Pending Publication Date: 2022-06-07
西北农林科技大学深圳研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention: kiwifruit bacterial canker is one of the most destructive diseases of kiwifruit, and its prevention and control agents are mainly copper preparation chemical agents at present, and the types of biological agents are limited and the control effect is not good. Restrictive factors that cannot be ignored in healthy development

Method used

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  • Paenibacillus polymyxa and application thereof in agriculture
  • Paenibacillus polymyxa and application thereof in agriculture
  • Paenibacillus polymyxa and application thereof in agriculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This example describes Paenibacillus polymyxa ( Paenibacillus polymyxa YLC1) separation, purification process and identification results.

[0034] 1. Isolation and purification of strains

[0035] Soil samples were collected from the rhizosphere of healthy kiwifruit plants in a kiwifruit orchard in Beiyang Village, Yangling District, Xianyang City, Shaanxi Province. Take 10 g of rhizosphere soil sample and put it into a sterilized 500 mL triangular flask, add sterilized steel balls with a diameter of 5 mm (the number of steel columns is preferably 5) and 200 mL of sterile water. Shake for 20 min on a shaking table with a rotational speed of 180 rpm, and obtain suspension A after standing.

[0036] Mix 1 mL of suspension A with 199 mL of sterile water under sterile conditions to obtain suspension B. Dip Suspension B with a sterilized toothpick, streak the LB plate, replace the toothpick after each streak, and continue streaking at the end of the streak to obtain a si...

Embodiment 2

[0054] In this example, Paenibacillus polymyxa was determined by the in vitro shoot method ( Paenibacillus polymyxa YLC1) Activity of fermentation broth against kiwifruit bacterial canker.

[0055] The 1-2 year old branches of the healthy plants of the kiwifruit Hongyang variety were collected, cut into small pieces of about 20 cm, washed with tap water, and then surface-sterilized with a 0.5% sodium hypochlorite aqueous solution, and finally rinsed with sterile water and dried. Both ends of the dried branches were sealed with molten paraffin, and the phloem of the branches was cut laterally to form a wound for later use.

[0056] Paenibacillus polymyxa ( Paenibacillus polymyxa YLC1) fermentation broth (the preparation method is the same as that of Example 1) was applied to the branches of kiwifruit, and the sterile fermentation medium was applied as a control. After being placed in a sandbox for 48 hours, the OD 600 The kiwifruit bacterial canker bacteria with a value of ...

Embodiment 3

[0062] This example gives Paenibacillus polymyxa ( Paenibacillus polymyxa Field control effect of YLC1) fermentation broth on kiwifruit bacterial canker.

[0063] The test kiwifruit variety was Hongyang, and the tree age was 7-8 years. The test site was Hongyang Kiwifruit Plantation, Yangling District, Xianyang City, Shaanxi Province. In the selected orchard, 2 chemical treatment plots were randomly divided. From the fruit picking in autumn to the pre-deciduous stage, use Paenibacillus polymyxa The fermentation broth of YLC1 was dried twice, and the interval between the two applications was 15 d. The 1000-fold dilution of 46% copper hydroxide water dispersible granules was used as a conventional treatment control, and the efficacy investigation was carried out in early March of the following year. Paenibacillus polymyxa ( Paenibacillus polymyxa The formula used in the evaluation method of the field control effect of YLC1) fermentation broth on kiwifruit bacterial canker ...

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Abstract

The invention provides a Paenibacillus polymyxa YLC1, and the preservation number of the Paenibacillus polymyxa YLC1 is CGMCC (China General Microbiological Culture Collection Center) No.24524. The invention further provides a preparation method of the Paenibacillus polymyxa YLC1. According to the present invention, the growth of Pseudomonas syringae pv.actinidia (Pseudomonas syringae pv.actinidia) can be inhibited by using the thallus and the fermentation broth of the Pseudomonas syringae pv.actinidia strain, such that the growth of the Pseudomonas syringae pv.actinidia can be inhibited; the strain disclosed by the invention can be used as a biocontrol bacterium to replace a conventional copper preparation, is used for preventing and treating kiwi fruit bacterial canker, and meanwhile, the strain is rapid in growth and reproduction, strong in adaptive capacity and non-toxic and harmless to people and livestock.

Description

technical field [0001] The invention belongs to the technical field of biological resource utilization, relates to a microbial strain and its antibacterial activity, in particular to a strain of Paenibacillus polymyxa and the use of the Paenibacillus polymyxa to prevent and treat kiwifruit bacterial canker. Background technique [0002] by Pseudomonas syringae kiwifruit pathogenic variety ( Pseudomonas syringae pv. actinidiae Bacterial canker (Kiwifruit Bacterial Canker) caused by Psa for short is one of the most devastating diseases of kiwifruit. Kiwifruit bacterial canker (also known as kiwifruit canker) can cause kiwifruit leaf spot, flower rot, cankers and pus on tree branches and even dead trees. At present, the prevention and treatment of kiwifruit canker is highly dependent on copper-based chemicals, but the extensive use of these chemicals not only reduces the sensitivity of pathogens to chemicals, but also puts a lot of pressure on the environment, which serious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01P1/00C12R1/01
CPCC12N1/20A01N63/25
Inventor 黄丽丽王华马嘉冰刘巍刘露
Owner 西北农林科技大学深圳研究院
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