Polypeptide and application thereof, antibacterial agent and preparation method of antibacterial agent

Abstract

The invention discloses a polypeptide and application thereof, an antibacterial drug and a preparation method thereof. The sequence of the amino acid main chain of the polypeptide is Lys-Lys-Lys-His-Lys-Lys-Lys or Arg-Arg-Lys-His-Lys-Arg-Arg, and the sequence of the amino acid main chain of the polypeptide is as shown in the specification. The polypeptide which is simple in sequence and structure and can be used for resisting bacteria is provided, the number of total amino acids is small, the preparation cost is reduced on the premise that good performance is guaranteed, the hydrophobic beta folding sequence is adopted as a side chain to drive the polypeptide to form a regular assembly structure, and the physiological stability of an assembly is enhanced. After the polypeptide is assembled with multiple components of metal ions and ligand drugs through coordination, the loading capacity is high, deep delivery of the drugs can be achieved, and the active targeting property on bacteria is shown.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and more specifically relates to a polypeptide and its application, antibacterial drugs and its preparation method. Background technique [0002] Polypeptides are a type of compound composed of α-amino acids as structural units and connected by peptide bonds according to a certain sequence. Because it is an intermediate product of protein hydrolysis, it is widely distributed in the living body and participates in the regulation of various physiological activities and functions in the living body. Peptides have the advantages of high efficacy, good bioavailability, small side effects, easy degradation and metabolism, and easy chemical modification. Cytokine mimetic peptides, antibacterial active peptides, diagnostic peptides, and other small medicinal peptides have been developed and widely used. In the field of medicine and cosmetics. [0003] Peptides used in the antibacterial fie...

AI Technical Summary

Problems solved by technology

[0003] Peptides used in the antibacterial field are usually obtained through genetic engineering expression systems, but they are easily decomposed by host cells, toxic to host cells, uneven amino acid composition, and expression efficiency Low, often expressed in the form of fusion protein, it is difficult to purify and separate in the later stage, which limits the wide application of antimicrobial peptides

In recent years, a method for preparing antibacterial polypeptides by solid-phase synthesis has emerged, which can solve the above problems. However, the sequence of antibacterial polypeptides prepared by solid-phase synthesis is relatively long and complicated, and there are many types of amino acids used. Usually, 5-6 types are used, or even more than 10. Such an antibacterial polypeptide sequence is usually a main chain without side groups, and its performance is adjusted by adjusting the sequence and quantity of different types of amino acids, which greatly increases the preparation cost of antibacterial polypeptides

However, drugs prepared from antibacterial peptides are usually directly wrapped with antibacterial peptides, which has a low loading capacity and cannot guarantee deep drug delivery.

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