Method for preparing copper nanocluster fluorescent probe and detecting hypochlorite in water environment
A fluorescent probe, hypochlorite technology, applied in nano-optics, nanotechnology, nanotechnology and other directions, can solve problems such as poor specificity of hydrophobic biological systems, and achieve lower detection limit of hypochlorous acid, high selectivity, and good detection. The effect of sensitivity
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[0051] A method for preparing a copper nanocluster fluorescent probe, comprising the following steps:
[0052] 0.05g / mL, pH 6~7 polyvinylpyrrolidone aqueous solution, 100mM ascorbic acid aqueous solution, 100mM copper source aqueous solution, the feeding ratio of polyvinylpyrrolidone, ascorbic acid, copper source is 1g:(1.5~2.5)×10 -4 mol:(1.5~2.5)×10 -5 mol, and then incubated at room temperature for 6 to 7 days. After dialysis treatment through a dialysis membrane with a molecular weight cut-off of 7,000 to 14,000, the cut-off solution was lyophilized to obtain copper nanocluster fluorescent probes.
[0053] A fluorescence detection method for hypochlorite in a water environment, comprising the following steps:
[0054] S1: Mix the copper nanocluster fluorescent probe with phosphate buffer to prepare an aqueous solution to obtain a concentration of 0.4 mgmL -1 the fluorescent probe solution;
[0055] S2: Mix the fluorescent probe solution and the solution to be tested eve...
Embodiment 1
[0065] Preparation of a copper nanocluster (CuNCs) fluorescent probe:
[0066] 1) Add 1g of polyvinylpyrrolidone (PVP) into a 100mL flask, add 20mL of ultrapure water, and ultrasonically treat until the PVP dissolves; then add an appropriate amount of sodium hydroxide solution (1.0M) dropwise until the pH of the solution is adjusted to 6.0;
[0067] 2) Mix 2 mL of 100 mM ascorbic acid (AA) aqueous solution with 0.2 mL of 100 mM copper sulfate pentahydrate (CuSO 4 ·5H 2 O) The aqueous solution was mixed and added to the solution obtained in step 1); after incubation at constant temperature (25°C) in a shaker for 6 days, the solution changed from initial colorless to clear and transparent pale yellow under sunlight, and the solution was exposed to UV light at 365 nm. Strong blue fluorescence can be observed under the illumination of the lamp, indicating the formation of CuNCs;
[0068] 3) A dialysis bag with a molecular weight cut-off of 7000 was used for a three-day dialysis ...
Embodiment 2
[0075] In this example, the effective detection range of the copper nanocluster fluorescent probe prepared in Example 1 to the hypochlorite in the water sample is characterized by the spectral titration test, and the specific process includes the following steps:
[0076] S1: CuNCs were added to phosphate buffer to obtain a concentration of 0.4 mg mL -1 Fluorescent probe solution; wherein, the concentration of phosphate buffer is 50mM, pH=6.2;
[0077] S2: adding hypochlorous acid to the CuNCs detection solution and mixing evenly to obtain a solution to be detected with a hypochlorous acid concentration of 0-8 μM;
[0078] S3: Using a fluorescence spectrometer at a wavelength of 340 nm, the fluorescent probe solution prepared in step S1 and the solution to be detected prepared in step S2 are used to collect signals, and the following results are obtained: Image 6 The fluorescence spectrum shown in the figure shows that the fluorescence intensity of CuNCs at 416 nm gradually ...
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