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Exosome complex liquid for treating alopecia and preparation method thereof

A technology of exosomes and compound liquids, which is applied in the direction of medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, can solve the problems of hair loss recurrence and strong dependence, achieve good hair growth, increase the number of hair follicles, Promotes blood vessel formation and circulation

Pending Publication Date: 2022-07-05
诺赛联合(北京)生物医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the direct cause of hair loss is that the hair follicle cells are in the telogen stage and the hair follicles degenerate. Therefore, how to restore the function of the hair follicles and promote the growth of hair is particularly critical; at present, the methods used for the treatment of hair loss include hair follicle transplantation, taking and external use of drugs , such as finasteride, etc., but the existing drugs are highly dependent, and once the drug is stopped, it is easy to cause hair loss recurrence

Method used

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  • Exosome complex liquid for treating alopecia and preparation method thereof
  • Exosome complex liquid for treating alopecia and preparation method thereof

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Embodiment 1

[0042] This embodiment is a preparation method of adipose-derived mesenchymal stem cell exosome solution, and the preparation method includes the following steps:

[0043] (a) obtaining human adipose tissue and shredding, and then using collagenase to carry out tissue digestion and centrifugation to obtain primary adipose mesenchymal stem cells;

[0044] (b) Primary adipose-derived mesenchymal stem cells were resuspended in serum-free medium (DMEM / F12 medium) and incubated at 37°C, 5% CO 2 Cultivated at 70%-85% saturation, remove the medium and wash, digest with trypsin, and then subculture;

[0045] (c) Collect the culture supernatants of the third and fourth passages, and then centrifuge at 4000×g for 20 min at 4°C to remove cells and cell debris. Then, centrifuge at 200,000×g at 4°C for 60 min to obtain adipose mesenchyme. Stem cell exosome precipitation;

[0046] (d) The adipose-derived mesenchymal stem cell exosome pellet was resuspended in PBS to obtain an 8 mg / ml adip...

Embodiment 2

[0048] The present embodiment is the extraction method of oriental orientalis leaf and Polygonum multiflorum extract, and this extraction method comprises the following steps:

[0049] (1) arborvitae leaf and Polygonum multiflorum are respectively pulverized to a mesh particle size of not less than 50 meshes to obtain pulverized material;

[0050] (2) According to the mass volume ratio of pulverized material and ethanol solution of 1:8, place pulverized material in 55% ethanol solution and extract for 2 hours at 200 r / min and 45°C, continuously extract for 3 times, and then extract the extract Mixing, concentrating, and freeze-drying are carried out to obtain the above-mentioned oriental orientalis leaf extract and Polygonum multiflorum extract respectively.

Embodiment 3

[0052] This embodiment is a preparation method of fibroblast extracellular matrix solution, and the preparation method includes the following steps:

[0053] (a) obtaining human skin tissue, then carrying out tissue digestion and centrifugation to obtain primary fibroblasts;

[0054] (b) use serum-free medium (DMEM / F12 medium) to resuspend primary fibroblasts and culture, digest the cells after reaching 80% saturation, and then carry out subculture;

[0055] (c) collecting the 3rd and 4th generation culture supernatants, then centrifuging to remove cells and cell debris, and then using ultracentrifugation to obtain fibroblast extracellular matrix precipitates;

[0056] (d) The fibroblast extracellular matrix pellet was resuspended in PBS to obtain 8 mg / ml fibroblast extracellular matrix solution.

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Abstract

The invention discloses an exosome complex liquid for treating alopecia and a preparation method thereof. The exosome complex liquid comprises the following components in percentage by weight: 20%-30% of an adipose tissue-derived stromal cell exosome solution, 8%-12% of a fibroblast extracellular matrix solution, 1%-3% of a cacumen platycladi extract, 2%-4% of a polygonum multiflorum extract and the balance of normal saline. The adipose-derived mesenchymal stem cell exosome, the fibroblast extracellular matrix and the three-type collagen in the exosome composite liquid can provide nutrient substances for hair follicle cells, meanwhile, can stimulate differentiation of hair follicle stem cells, increase the number of hair follicles, stimulate hair follicles in a resting period to be converted into a growing period, and improve the hair follicle growth period. And a cacumen platycladi extract, a radix polygoni multiflori extract, amino acid and multivitamin components which are used for promoting blood circulation, resisting inflammation, removing fat and regulating immunity are supplemented, so that formation and circulation of blood vessels around hair follicles are promoted, and better hair strengthening and growing effects are achieved.

Description

technical field [0001] The invention relates to the technical field of exosome application, in particular to an exosome compound solution for treating hair loss and a preparation method thereof. Background technique [0002] Hair loss is the phenomenon of hair falling out. The hair that normally falls off is the hair in the degenerative and telogen phases. Since the hair entering the degenerative phase and the newly entering the growing phase are constantly in a dynamic balance, a normal number of hairs can be maintained. Pathological alopecia refers to abnormal or excessive hair loss. There are many reasons for it, including hormonal alopecia, congenital alopecia, and nutritional alopecia. [0003] However, the direct cause of hair loss is that the hair follicle cells are in the resting phase and the hair follicle degenerates. Therefore, how to restore the function of the hair follicle and promote the growth of hair is particularly critical. At present, the treatment metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/704A61K35/33A61K35/28C12N5/0775A61P17/14
CPCA61K35/28A61K35/33A61K36/14A61K36/704C12N5/0667A61P17/14C12N2509/00C12N2509/10A61K2236/333A61K2300/00
Inventor 李少波亓爱杰马润林李立华
Owner 诺赛联合(北京)生物医学科技有限公司
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