Preparation method of ampelopsis grossedentata extract

A rattan tea extract and rattan tea technology are applied in the field of preparation of rattan tea extracts, which can solve the problems of destroying biological active substances, solvent residues, and reducing the content of rattan tea effective substances, and achieve low temperature, stable properties and product stability. good effect

Pending Publication Date: 2022-07-05
HUBEI CHINA TOBACCO IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of low-temperature countercurrent leaching, and then adding surfactants not only has the problem of solvent residues, but the surfactants may also destroy the biologically active substances in the raw materials, thereby reducing the content of effective substances in vine tea.

Method used

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  • Preparation method of ampelopsis grossedentata extract

Examples

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Comparison scheme
Effect test

Embodiment 1

[0042] A preparation method of vine tea extract, comprising the following steps:

[0043] S1. Weigh 40g of rattan tea, add 400g of deionized water, then add 0.4g of combined enzymes for enzymolysis for 40min, and filter to obtain filter residue A and filtrate A, wherein the combined enzymes include pectinase, cellulase, amylase, protease The mass ratio is 1:1:1:1;

[0044] S2, get the filter residue A obtained in the above-mentioned S1 and dissolve it in 400g deionized water, add 0.8g citric acid-disodium hydrogen phosphate, adjust the pH value to be 2.5, high-pressure microwave treatment, filter to obtain filter residue B and filtrate B, wherein, high pressure The pressure of microwave treatment is 400KPa, and the time is 10 min;

[0045] S3, get the filter residue B obtained in above-mentioned S2 and be dissolved in 400g deionized water, add 0.8g citric acid-disodium hydrogen phosphate, adjust pH value to be 8, high pressure microwave treatment, filter to obtain filter resi...

Embodiment 2

[0050] A preparation method of vine tea extract, comprising the following steps:

[0051] S1. Weigh 40g of vine tea, add 400g of deionized water, then add 1.2g of combined enzymes for enzymolysis for 40min, and filter to obtain filter residue A and filtrate A, wherein the combined enzymes include pectinase, cellulase, amylase, protease The mass ratio is 1:1:1:1;

[0052] S2, get the filter residue A obtained in above-mentioned S1 and be dissolved in 400g deionized water, add 0.8g citric acid-disodium hydrogen phosphate, adjust the pH value to be 3, high-pressure microwave treatment, filter to obtain filter residue B and filtrate B, wherein, high pressure The pressure of microwave treatment is 400KPa, and the time is 10 min;

[0053] S3, get the filter residue B obtained in above-mentioned S2 and be dissolved in 400g deionized water, add 0.8g citric acid-disodium hydrogen phosphate, adjust the pH value to be 8.5, high pressure microwave treatment, filter to obtain filter resid...

Embodiment 3

[0058] A preparation method of vine tea extract, comprising the following steps:

[0059] S1, weigh 40g of vine tea, add 400g of ethanol solution with a mass concentration of 60%, then add 1.2g combined enzyme enzymolysis for 40min, filter to obtain filter residue A and filtrate A, wherein, the combined enzyme includes pectinase, cellulase, The mass ratio of amylase and protease is 1:1:1:1;

[0060] S2, get the filter residue A obtained in above-mentioned S1 and be dissolved in 400g deionized water, add 0.8g citric acid-disodium hydrogen phosphate, adjust the pH value to be 3, high-pressure microwave treatment, filter to obtain filter residue B and filtrate B, wherein, high pressure The pressure of microwave treatment is 400KPa, and the time is 10 min;

[0061] S3, get the filter residue B obtained in above-mentioned S2 and be dissolved in 400g deionized water, add 0.8g citric acid-disodium hydrogen phosphate, adjust the pH value to be 8.5, high pressure microwave treatment, ...

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Abstract

The invention discloses a preparation method of ampelopsis grossedentata extract, which comprises the following steps: preliminarily extracting ampelopsis grossedentata through enzymolysis, simultaneously destroying intercellular layers and cell walls among ampelopsis grossedentata cells, further destroying cell inner layers, simulating an environment in which oral medicines are transferred and absorbed through gastrointestinal tracts, and carrying out acid-base digestion treatment on ampelopsis grossedentata filter residues, so as to obtain the ampelopsis grossedentata extract. The method comprises the following steps: firstly, extracting active ingredients in ampelopsis grossedentata, so that the active ingredients in the ampelopsis grossedentata are leached very easily, then fully extracting the active ingredients in the ampelopsis grossedentata in a short time under a low-temperature condition by adopting an ultrasonic-assisted technology and damaging cell walls of raw materials through a mechanical effect, a cavity effect and a heat effect generated by ultrasonic waves, and finally adding high-concentration alcohol to extract for a certain time, negative action components such as protein and pectin in the extract are removed, and the yield of active components such as flavone and polysaccharide of ampelopsis grossedentata is increased to a greater extent. The ampelopsis grossedentata extract prepared by the method is bright in color, palatable in sourness and sweetness and good in product stability.

Description

technical field [0001] The invention relates to the technical field of rattan tea extraction, in particular to a preparation method of rattan tea extract. Background technique [0002] Ampelopsis grossedentata, scientific name Ampelopsis grossedentata, is a wild vine of the genus Ampelopsis, commonly known as manna tea, white tea, white haired monkey, etc. It is a perennial vine widely distributed in the south of the Yangtze River in China. It is an ancient medicinal and edible plant and has been widely used in the folk as a tea plant and Chinese herbal medicine for a long time. It is a kind of wild natural tea plant substitute tea with unique health care effect and drinking value. The whole plant has medicinal value. In addition, vine tea is cool in nature, and has high medicinal and nutritional value such as hemostasis, removing blood stasis and swelling, removing rheumatism and inflammation, antithrombotic, blood lipid lowering and antioxidant. The tender stems and leave...

Claims

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Application Information

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IPC IPC(8): A61K36/87A61K9/00A61P39/06A61P31/04A61P31/10
CPCA61K36/87A61K9/0053A61P39/06A61P31/04A61P31/10A61K2236/19A61K2236/331A61K2236/333A61K2236/53A61K2236/51A61K2236/39
Inventor 邵佩庄虎蹇顺华尹团章吴昭
Owner HUBEI CHINA TOBACCO IND
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