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Bacillus pseudomycoides and application of bacillus pseudomycoides in preparation of phytopathogen bacteriostatic agent

A technology of plant pathogenic bacteria and Bacillus, applied in the field of microorganisms, can solve the problems of increasing crop absorption and achieve good antibacterial effect

Pending Publication Date: 2022-07-05
山东野田生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that Bacillus pseudomycoides can produce plasmin and increase the absorption of potassium by crops, but there are few reports on the antibacterial effect of this bacteria

Method used

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  • Bacillus pseudomycoides and application of bacillus pseudomycoides in preparation of phytopathogen bacteriostatic agent
  • Bacillus pseudomycoides and application of bacillus pseudomycoides in preparation of phytopathogen bacteriostatic agent
  • Bacillus pseudomycoides and application of bacillus pseudomycoides in preparation of phytopathogen bacteriostatic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Isolation and identification of Bacillus pseudomycoides:

[0019] 2.5 g of soil samples from Pucheng County, Weinan City, Shaanxi Province were placed in 50 ml of sterile water, shaken at 30°C and 250 rpm for 1 h, and then 500 μl of soil suspension was taken for gradient dilution in a PA bottle filled with 4.5 ml of sterile water. take 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 The soil suspensions of these dilutions were added to the medium for coating, and the growth of the plate colonies was observed after culturing for 24h, 48h, 72h, and 96h. Bacteria with high antibacterial rate and broad antibacterial spectrum were selected; Bacillus pseudomycides FJH2 was finally screened.

[0020] (1) Morphological characteristics

[0021] The isolated pseudomycobacterium, the colony morphology on LB solid medium is as follows: figure 1 As shown, the colonies are off-white flattened roots.

[0022] (2) Physiological and biochemical experiments

[0023] Physiological and bioc...

Embodiment 2

[0030] Tolerance test for Bacillus pseudomycoides:

[0031] (1) pH range resistance test:

[0032] The seed solution of Bacillus pseudomycides was inoculated into LB medium with different initial pH according to the inoculum amount of 2%, and cultured at 30 °C and 200 r / min. After culturing for 16 h, samples were taken to measure OD. 600 . The initial pH gradient of the medium was set to 3, 4, 5, 6, 7, 8, 9, 10 (initial OD was 0).

[0033] LB medium: 10 g of peptone, 5 g of yeast extract, 10 g of NaCl, 1000 mL of distilled water, pH 7.0, sterilized by autoclaving at 121°C for 20 min.

[0034] PH 3 4 5 6 7 8 9 10 OD 600

0 0 0 4.712 5.356 4.504 0 0

[0035] (2) Salt tolerance test:

[0036] The seed solution of Bacillus pseudomycides was inoculated into LB medium with different salt concentrations according to the amount of 2%, and cultured at 30 °C and 200 r / min. After culturing for 16 hours, samples were taken to measure OD. 600 . T...

Embodiment 3

[0043] Inhibitory effect of Bacillus pseudomycides on plant pathogens:

[0044] The inhibition zone method was used to determine the inhibitory ability of Bacillus pseudomycides against pathogenic bacteria. Bacillus pseudomycoides was activated with LB, and the seed solution was appropriately diluted and spread on a PDA plate, and sterile water was used as a control group. Punch holes with a pipette tip on a PDA plate covered with pathogenic fungi, pick the fungal block and inoculate it upside down on a PDA plate coated with Bacillus pseudomycides, place the plate at 37°C for 4 days, and measure the diameter of the fungal colony. Calculate the bacteriostatic rate to analyze the bacteriostatic effect.

[0045] The plant pathogens selected in this experiment include Fusarium oxysporum F.sp. Cubense, Fusarium oxysporum, Penicillium brasilianum, Fusarium solani, Fusarium Fusariumchlamydosporum), Penicilliumglaucum and Mucor.

[0046] PDA medium: potato 200g, glucose 20g, distil...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus pseudomycoides FJH2 and application of the bacillus pseudomycoides FJH2 in preparation of a plant pathogen bacteriostatic agent, and the preservation number of the bacillus pseudomycoides FJH2 is CCTCC NO: M2022049. The strain has the functions of resisting phytopathogen and the like; the strain can grow under the conditions that the pH is 6.0-pH 8.0, the salt concentration is 1%-6% and the temperature is 20-40 DEG C, the inhibition effect on several plant pathogenic bacteria is good, the inhibition rate on fusarium oxysporum reaches up to 91.47%, and the strain has potential application value in preparation of biocontrol bacteria.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bacillus pseudomycoides and its application in the preparation of a bacteriostatic agent for plant pathogenic bacteria. Background technique [0002] Fusarium is widely present in soil and plants, mainly acting on the junction of plant roots and rhizomes, destroying its vascular tissue, and producing enzymes, phenols and other metabolites in the metabolic process, affecting the growth and development of host plants. Plant fusarium wilt occurs most seriously in summer, and the infected plants are mainly manifested as stunted growth, yellow leaves, withered leaves and dead leaves, and rhizomes rotten. At present, there is no very effective chemical pesticide for the treatment of fusarium wilt in agriculture, so the treatment of fusarium wilt mainly relies on biological control methods. Studies have shown that Bacillus pseudomycides can produce plasmi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/22
Inventor 黄丽陈振民闫训博朱秋艳齐红跃冯克耿月峰张新龙贾忠清苏善柱杨少周刘永高丁祥祺
Owner 山东野田生物科技有限公司
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