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Microsphere frozen section as well as preparation method and application thereof

A technology of frozen section and microspheres, which is applied in microsphere preparation, microcapsule preparation, material analysis using wave/particle radiation, etc. It can solve problems such as lack of flatness, hole deformation, unsuitable porosity, etc. The effect of rolling, not easy to fragment, and shortening the soaking time

Pending Publication Date: 2022-07-05
SUZHOU CANCERCELL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the cross-sections of microspheres in the scanning electron microscope pictures given by many literatures have problems such as lack of flatness and pore deformation, which are not suitable for the calculation of porosity; The slicing method makes perfect sense

Method used

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  • Microsphere frozen section as well as preparation method and application thereof
  • Microsphere frozen section as well as preparation method and application thereof
  • Microsphere frozen section as well as preparation method and application thereof

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Embodiment 1

[0043] The present embodiment provides a polylactic acid and polyglycolic acid microsphere cryosection and a preparation method thereof. The preparation method of the microsphere cryosection comprises the following steps:

[0044] (1) Preparation of microspheres:

[0045]Dissolve bovine serum albumin and sodium chloride in a certain concentration of poloxamer 188 solution to obtain an inner water phase. The inner water phase is added to the dichloromethane solution of polylactic acid and polyglycolic acid, and stirred at a high speed under low temperature conditions to form a primary emulsion. The colostrum was added to the polyvinyl alcohol solution and stirred for 10 min to form a double emulsion. The double emulsion is transferred to a large beaker containing a polyvinyl alcohol solution and slowly stirred to solidify the microspheres, collected by centrifugation, washed three times with water, and freeze-dried to prepare the microspheres.

[0046] (2) preparation of gela...

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Abstract

The invention provides a microsphere frozen section as well as a preparation method and application thereof. The preparation method of the microsphere frozen section comprises the following steps: dissolving bovine serum albumin and a pore-foaming agent in a poloxamer 188 solution to obtain an inner water phase; adding the mixture into an oil phase containing polylactic acid and polyglycolic acid, and stirring to form a primary emulsion; then adding into an outer water phase, stirring to form a multiple emulsion, curing, centrifugally collecting, and freeze-drying to obtain microspheres; then, suspending in a gelatin-glycerol embedding medium for soaking treatment, and freezing and solidifying to obtain an embedded block; and slicing the microspheres to obtain frozen microspheres. The method disclosed by the invention has the characteristics of simplicity, rapidness, no need of repairing an embedding block, low consumable price, difficulty in fragmentation, easiness in sheet expansion and the like, and the prepared microspheres have flat sections and clear pore structures, are suitable for observation of a scanning electron microscope, and can be used for calculating the porosity of the microspheres by image processing software; the frozen microballoon slice also provides reference for the slicing technology of microballoons, polymer porous scaffolds and the like prepared from other materials.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a microsphere frozen section and a preparation method and application thereof; in particular, it relates to a polylactic acid polyglycolic acid (polylactic acid polyglycolic acid) (polylactic acid and polyglycolic acid (BSA) loaded with bovine serum albumin (BSA) embedded in a freezing embedding agent). PLGA) cryosection of microspheres. Background technique [0002] Polylactic polyglycolic acid (PLGA) microspheres have been widely studied and applied in the field of drug sustained and controlled release due to their safety, non-toxicity and biodegradability. Drug release behavior is one of the important indicators for evaluating microsphere preparations, and the internal structure and drug distribution of microspheres are closely related to their drug release behavior. Therefore, studying the internal structure of microspheres is helpful to explore the drug release me...

Claims

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Application Information

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IPC IPC(8): G01N23/2251G01N23/2202B01J13/02
CPCG01N23/2251G01N23/2202B01J13/02
Inventor 王守立彭金玉吴月
Owner SUZHOU CANCERCELL BIOTECH
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