Composite plant source polypeptide as well as preparation method and application thereof

A plant source and drug technology, applied in the field of biomedicine, can solve problems such as unreported, and achieve the effects of reducing blood lipids, effectively regulating blood sugar, and improving antioxidant capacity.

Pending Publication Date: 2022-07-08
HENAN UNIV OF URBAN CONSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is more common to use mulberry leaf and bitter melon extracts directly for hypoglycemic drugs, but there is no report on

Method used

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  • Composite plant source polypeptide as well as preparation method and application thereof
  • Composite plant source polypeptide as well as preparation method and application thereof
  • Composite plant source polypeptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This example provides a preparation test of a compound plant-derived polypeptide.

[0025] The preparation method of ginseng polypeptide includes: taking 20g of fresh ginseng, adding 200mL of 0.01mol / L PBS (pH=7), leaching for 12-48 hours, taking the supernatant and adding 400mL ice pre-cooling acetone to precipitate, and mixing well After standing overnight, after centrifugation, the precipitate was taken out and dissolved in 200 mL of 0.01mol / L PBS (pH=7), and then freeze-dried to obtain crude ginseng protein; The 30KDa ultrafiltration is concentrated, the filtrate is collected, and then the 10KDa ultrafiltration is performed to collect the effluent with a molecular weight of less than 10KDa, and freeze-dried to obtain the ginseng polypeptide.

[0026] The preparation method of mulberry leaf polypeptide includes: taking fresh mulberry wood, washing it to remove impurities, drying it, and pulverizing it to 20 meshes to obtain mulberry leaf powder. At room temperature,...

Embodiment 2

[0030] This example provides a preparation test of a compound plant-derived polypeptide.

[0031] The preparation method of this example is the same as that of Example 1, except that, in parts by mass, the compound plant-derived polypeptide described in this example includes component A and component B, and the ginseng polypeptide and mulberry leaf polypeptide in component A are ginseng polypeptide and mulberry leaf polypeptide. The ratio of the compound plant-derived polypeptide is 10:1; the ratio of the component A and the component B in the composite plant-derived polypeptide is 4:1. After mixing according to the above ratio, it is the composite plant-derived polypeptide.

Embodiment 3

[0033] This example provides a preparation test of a compound plant-derived polypeptide.

[0034] The preparation method of this example is the same as that of Example 1, except that, in parts by mass, the compound plant-derived polypeptide described in this example includes component A and component B, and the ginseng polypeptide and mulberry leaf polypeptide in component A are ginseng polypeptide and mulberry leaf polypeptide. The ratio of the compound plant-derived polypeptide is 3:1; the ratio of the component A and the component B in the composite plant-derived polypeptide is 4:1. After mixing according to the above ratio, it is the composite plant-derived polypeptide.

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Abstract

The invention provides a composite plant-derived polypeptide and a preparation method and application thereof.The composite plant-derived polypeptide comprises a component A and a component B. The component A comprises ginseng polypeptide and folium mori polypeptide, and the component B is a bitter gourd extract; the mass ratio of the ginseng polypeptide to the mulberry leaf polypeptide in the component A is (16: 1)-(1: 2); the ratio of the component A to the component B in the composite plant source polypeptide is 4: 1. Within a certain matching range, the composite plant source polypeptide has excellent oxidation resistance, blood sugar reducing capacity and blood fat reducing capacity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a compound plant-derived polypeptide and a preparation method and application thereof. Background technique [0002] Ginseng (Panax giseng C.A.Mey.) has a very special position in the history of Chinese medicine, known as the "King of Herbs", and is my country's iconic Chinese herbal medicine. Modern medical research has proved that ginseng has a good protective effect on the central nervous system, cardiovascular and cerebrovascular system, respiratory system, blood and hematopoietic system, endocrine system and reproductive system. In addition, studies have shown that ginseng also has obvious anti-tumor, anti-radiation, anti-aging and detoxification functions. [0003] Mulberry leaves (Folium Mori) are the dried leaves of Morus mulberry. Mulberry leaves contain ecdysterone, lupine ketone, target fragrant glycosides, mulberry glycosides and many other amino acids, vitamins, ...

Claims

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Application Information

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IPC IPC(8): A61K38/02A61K38/01A61K36/42A61P3/10A61P3/06A61P39/06
CPCA61K38/02A61K38/011A61K36/42A61P3/10A61P3/06A61P39/06A61K2300/00
Inventor 尹国友孙婕
Owner HENAN UNIV OF URBAN CONSTR
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