Preparation method of salmonella enteritidis colloidal gold immunochromatography test strip

Abstract

The invention belongs to the technical field of immunodetection, and discloses a preparation method of a salmonella enteritidis colloidal gold immunochromatography test strip, which comprises the following steps: preparing colloidal gold by using an optimized Tris-base auxiliary method, and coupling the colloidal gold with a salmonella enteritidis monoclonal antibody to obtain an immune label; and assembling the screened sample pad, the combination pad fixed with the immune label, the NC membrane fixed with the T / C line antibody, absorbent paper and PVC (polyvinyl chloride) to obtain the salmonella enteritidis colloidal gold immunochromatography test strip. Colloidal gold with better uniformity and stability is prepared on the basis of a Tris auxiliary method, the salmonella enteritidis colloidal gold immunochromatographic test strip established by taking the colloidal gold as a marker is high in sensitivity, good in specificity and good in repeatability, all properties meet the requirements of clinical rapid detection, and the salmonella enteritidis colloidal gold immunochromatographic test strip has good application prospects. It is proved that the salmonella enteritidis colloidal gold immunochromatography test strip prepared based on the Tris-assisted method can be used for clinical field detection.

Description

technical field [0001] The invention belongs to the technical field of immunodetection, and in particular relates to a preparation method of a Salmonella Enteritidis colloidal gold immunochromatographic test strip. Background technique [0002] At present, Salmonella Enteritidis is a common zoonotic enteric pathogen and the main pathogen causing acute gastroenteritis. It can cause clinical symptoms such as diarrhea, vomiting, and fever in livestock, poultry and humans, and in severe cases, it can cause sepsis, dehydration, and death. Salmonella Enteritidis is also the main cause of bacterial food poisoning in recent years, threatening human health and causing great economic losses to the country. Therefore, it is very important to establish a method for rapid detection of Salmonella Enteritidis. [0003] Salmonella belongs to Enterobacteriaceae, Gram-negative Enterobacteriaceae, the bacterium is oval in shape, with a size of (0.6-0.9) × (1-3) microns, usually without capsule...

AI Technical Summary

Problems solved by technology

The gold particles commonly used in traditional colloidal gold immunochromatography are all prepared by the Frens method, but the colloidal gold prepared by this method has poor stability and the prepared test strips have low sensitivity.

[0005] Through the above analysis, the problems and defects of the prior art are: the colloidal gold immunochromatography test strip prepared by the prior art has poor stability and low sensitivity; lower sex

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