Medicinal extract for preventing and treating diabetes and application thereof

An extract and diabetes technology, applied in the field of medicine, can solve the problems of prevention and treatment of type 2 diabetes drugs or health care products that have not been reported, and achieve high development prospects, simple extraction process, and significant hypoglycemic activity

Pending Publication Date: 2022-07-12
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of sparrow mouth tea or its extracts in the prepa

Method used

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  • Medicinal extract for preventing and treating diabetes and application thereof
  • Medicinal extract for preventing and treating diabetes and application thereof
  • Medicinal extract for preventing and treating diabetes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1

[0033] Preparation of Quezui Tea Extract.

[0034]Quezui tea was harvested from Wuding County, Yunnan Province. After processing, 1.7kg dried Quezui tea was obtained. It was extracted 3 times with 10 times hot water for 30 minutes each time. The filtrate was combined to obtain 20.8L, and the remaining Quezui tea residue was extracted with ethanol. 3 times, 2h each time, to obtain Quezui tea ethanol extract m 0 . 20.8L water extraction filtrate was concentrated to 12.6L, after D 101 Gradient elution of polystyrene macroporous adsorption resin, of which 100% of the water washing is the extract of Quezui tea m 1 , 25% ethanol eluate is Quezui tea extract m 2 , 100% ethanol eluate is Quezui tea extract m 3 .

Example Embodiment

[0035] Example 2

[0036] A 3T3-L1 adipocyte insulin resistance model was established to observe the effect of Quezui tea extract on glucose uptake in 3T3-L1 adipocytes.

[0037] 1. Induction and differentiation of 3T3-L1 cells

[0038] 3T3-L1 preadipocytes were placed in High glucose DMEM (10% newborn calf serum) at 37°C, 5% CO 2 cultured in a carbon dioxide incubator. After the cells grow and fuse, add 0.5mM IBMX, 1×10 -3 mg·L-1 insulin, 1×10 -3 mM dexamethasone, 1×10 -2 High glucose DMEM (10% fetal bovine serum) in mM rosiglitazone was cultured for 72h; then replaced with 1×10 -3 High glucose DMEM (10% fetal bovine serum) containing mg·L-1 insulin was further cultured for 24h. The successfully differentiated 3T3-L1 adipocytes became larger in size, and cyclic lipid droplets were aggregated around the nucleus.

[0039] 2. Experimental grouping and administration

[0040] The induced differentiated 3T3-L1 adipocytes were collected, digested with 1‰ collagenase-0.25% t...

Example Embodiment

[0052] Example 3

[0053] Detection of the inhibitory effect of Quezui tea extract on α-glucosidase.

[0054] The sparrow tea extract m 0 , m 1 , m 2 , m 3 The final concentrations after administration were 100, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.7813, 0.3906, 0.1953 μg / mL with PBS, respectively. Before the experiment, 10 μL of different concentrations of sample solutions and 50 μL of α-glucosidase solution (0.1 U / mL) were added to 100 μL of phosphate buffer (0.1 M), and incubated at 37 °C for 15 min. Immediately after the pre-incubation, 40 μL of 4-nitroα-D glucopyranoside (PNPG, 5 mM) was added and incubated at 37° C. for 60 min. After incubation with 50 μL Na 2 CO 3 The solution (0.1M) terminated the reaction, the OD value of the sample was measured at 405nm, and the α-glucosidase inhibition rate of each group of samples was calculated according to formula 1. Taking drug-free PBS as a blank control (adding the same amount of DMSO solution), three sub-wells were se...

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Abstract

The invention provides a medicinal extract for preventing and treating diabetes and application thereof, and belongs to the technical field of biological medicine. According to the present invention, the new tender leaves on the tip of the sparrow beak tea are adopted as the raw material, the dry sample sparrow beak tea is processed to obtain the dry sample sparrow beak tea, and the arbutin, the alpine glycoside, the monotonin and other substances can be rapidly enriched through the hot water extraction and the ethanol elution; experimental results show that the lysimachia foenum-graecum has a remarkable effect on improvement of type 2 diabetes mellitus. In the embodiment of the invention, when m0, m2 and m3 of the sparrow beak tea extracts act for 24 hours under the dosage of 80mu g/mL, 3T3-L1 fat cells can be obviously promoted to uptake glucose, and meanwhile, the sparrow beak tea extracts have an obvious inhibition effect on alpha-glucosidase; the raw materials are rich in source, the extraction process is simple, green and environment-friendly, the action effect is remarkable, and the traditional Chinese medicine composition has a potential effect in preparation of medicines or health-care products for preventing and treating type 2 diabetes and is worthy of industrial production and market popularization and application.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a medicinal extract for preventing and treating diabetes and its application, more particularly, to a Quezui tea extract and its application in the preparation of medicines or health products for preventing and treating type 2 diabetes. Background technique [0002] Type 2 diabetes is a high-consumption metabolic disease characterized by glucose and lipid metabolism disorders and absolute or relative insulin deficiency, and is accompanied by serious complications, mainly including macrovascular disease and microvascular disease. There are currently 415 million people with diabetes in the world, of which type 2 diabetes accounts for more than 90% of the total diabetic patients. Long-term type 2 diabetes has a great impact on people's psychology and physiology, and poses a serious threat to human health and public health. At present, clinical hypoglycemic drugs such as acarbose and ...

Claims

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Application Information

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IPC IPC(8): A61K36/45A23L33/105A61P3/10A61K127/00
CPCA61K36/45A61P3/10A23L33/105A61K2236/331A61K2236/39A61K2236/53A61K2236/51A61K2236/55A23V2002/00A23V2200/328A23V2250/21
Inventor 张于张玉梅郝小江李胜
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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