Method and application of preparing total triterpenes with hypoglycemic effect from Cyclocarya paliurus leaves

A technology for total triterpenes and hypoglycemia, which is applied in the field of medicine and food, and can solve problems such as no research or report on the preparation process of total triterpenes

Active Publication Date: 2021-10-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research or report about the preparation process for the preparation of total triterpenes with the polyamide material normal phase elution system as the core disclosed in this application

Method used

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  • Method and application of preparing total triterpenes with hypoglycemic effect from Cyclocarya paliurus leaves
  • Method and application of preparing total triterpenes with hypoglycemic effect from Cyclocarya paliurus leaves
  • Method and application of preparing total triterpenes with hypoglycemic effect from Cyclocarya paliurus leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Dry 5 kg of Cyclocarya paliurus leaves, grind into powder, and extract twice at room temperature with 50 L of 80% ethanol solution, each time for 5 days. The combined extracts were concentrated under reduced pressure to dryness to obtain a total extract of 750 g. The total extract was dispersed with 1 L of water, and extracted with petroleum ether and chloroform in equal volumes respectively to obtain 280 g of the chloroform extract. The chloroform extraction part was eluted by a 60-80 mesh polyamide chromatographic column (dichloromethane: methanol = 15:1), and the eluted part was collected to obtain an extract. Then dissolve the extract with water, adsorb on the AB-8 macroporous resin column, first wash away impurities with 30% ethanol, and then elute with 60% ethanol. The 60% ethanol eluate was collected and concentrated to dryness under reduced pressure to obtain 105 g of dry powder of total triterpenes, with a total extraction rate of 2.1%. The total triterpene c...

Embodiment 2

[0022] 10 kg of dried Cyclocarya paliurus leaves were powdered and refluxed with 80 L of 70% ethanol solution twice for 2 hours each time. The combined extracts were concentrated under reduced pressure to dryness to obtain 1600 g of the total extract. The total extract was dispersed with 2L of water, extracted successively with equal volumes of petroleum ether and ethyl acetate to obtain 560 g of ethyl acetate extract. The ethyl acetate extraction part was eluted by a 100-200 mesh polyamide column (petroleum ether: acetone = 1:1), and the eluted part was collected to obtain an extract. Then dissolve the extract with water, adsorb on D101 macroporous resin column, first wash away impurities with 40% ethanol, and then elute with 70% ethanol. The 70% ethanol eluate was collected and concentrated to dryness under reduced pressure to obtain 280 g of dry powder of total triterpenes, with a total extraction rate of 2.8%. The measured triterpene content was 85.4%.

Embodiment 3

[0024] Dry 1 kg of Cyclocarya paliurus leaves, powder them, and extract twice with 10 L of 90% ethanol under reflux, each time for 100 min. The combined extracts were concentrated under reduced pressure to dryness to obtain a total extract of 182 g. The total extract was dispersed with 500 mL of water, extracted successively with equal volumes of petroleum ether and ethyl acetate: n-butanol (10:1), to obtain 65 g of ethyl acetate-n-butanol extract. The extracted part was eluted by a 100-200 mesh polyamide column (dichloromethane: ethyl acetate = 2:1), and the eluted part was collected to obtain the total triterpene extract. Then dissolve the extract with water, adsorb on the DM130 macroporous resin column, first wash away impurities with 50% ethanol, and then elute with 90% ethanol. The 90% ethanol eluate was collected and concentrated to dryness under reduced pressure to obtain 25 g of dry powder of total triterpenes with a total extraction rate of 2.5%. The measured triter...

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Abstract

The invention provides a method for preparing total triterpenoids with hypoglycemic effect from the leaves of Cyclocarya paliurus, using the leaves of Cyclocarya paliurus as raw materials, extracting them with a mixed solvent of ethanol and water, extracting them with an organic solvent liquid-liquid, and passing through polyamide column chromatography Organic solvent elution and macroporous resin column chromatography ethanol water elution for separation and purification can obtain high-purity total triterpenes. Studies have shown that the total triterpenes and their main components arjunalic acid, asiatic acid, penicillic acid B, and compound 4 can effectively increase the glucose uptake of 3T3‑L1 adipocytes, and have antidiabetic activity. The total triterpenes and their main components are used to prepare products such as anti-diabetic drugs for lowering blood sugar, health food and functional food. The present invention develops a preparation method of total triterpenes in Cyclocarya paliurus leaves and the application of compound 4 in the preparation of antidiabetic drugs, which provides a basis for the popularization, utilization and deep processing of Chinese medicinal materials, and provides a new application direction.

Description

technical field [0001] The invention belongs to the technical field of medicine and food, and in particular relates to a method for preparing high-purity total triterpenes with hypoglycemic effect from Cyclocarya paliurus leaves, and the use of total triterpenes in the preparation of antidiabetic drugs, health food and functional food and other products in the application. Background technique [0002] Cyclocarya paliurus leaves are dried leaves of Cyclocarya paliurus (Batalin) Iljinsk. Cyclocarya paliurus is a unique single-species plant in my country, widely distributed in Jiangxi, Zhejiang, Anhui, Hunan, Guangdong and other areas south of the Yangtze River. Folks use its leaves as a tea drink. The tea tastes sweet and has the effects of promoting body fluids and quenching thirst, clearing heat and relieving summer heat, lowering blood sugar, lowering blood pressure and prolonging life. At the end of 2013, green willow leaves were approved by the National Health and Fami...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/19A61K36/52A61P3/10A23L33/105A61K127/00
CPCA23V2002/00A61K36/52A61K2236/333A61K2236/39A61K2236/51A61K2236/53A23L33/105A61P3/10A23V2200/328A23V2250/21
Inventor 甘礼社周长新方祝君莫建霞何钢锋
Owner ZHEJIANG UNIV
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