Biological agent for improving meat quality as well as preparation method and application of biological agent
A biological preparation and high-quality technology, which is applied in the preparation of sugar derivatives, the preparation and application of organic compounds, etc., to achieve the effect of promoting chest muscle deposition, improving the effect, and being convenient to use
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Embodiment 1
[0033] Obtaining and identification of plant extracts
[0034] 1. Extraction and identification of hesperidin
[0035] Hesperidin was extracted by solvent extraction. The dried tangerine peel was crushed and passed through a 250-mesh separation sieve to extract the active ingredients. The samples were stored at -20°C for later use. The specific steps are: (1) Weigh 0.20g of sieved dried tangerine peel powder into a 25mL polytetrafluoroethylene-lined stainless steel reaction kettle, and add 60% of tangerine peel according to a material-to-liquid ratio of 70:1 (g:mL). (volume fraction) ethanol solution, hot solvent extraction was carried out at 120°C, and the extraction time was 45min. (2) Centrifuge at 4000 r / min for 5 min after extraction, remove ethanol by rotary evaporation immediately, and store the remaining samples in vacuum freezer for subsequent identification.
[0036]Identification of hesperidin High performance liquid chromatography (HPLC) was used to quantitative...
Embodiment 2
[0045] Test design
[0046] Using a completely randomized experimental design, 200 healthy white-feathered broiler (AA) broiler chicks with an average body weight of (44.65±1.45) g were selected and randomly divided into 4 groups, namely the control group (Con Group), the orange Peridin group (HesGroup), rosmarinic acid group (Ros-A Group) and hesperidin-rosmarinic acid group (Hes x Ros-A Group), 5 replicates each, 10 chickens per replicate, There was no significant difference in body weight among the groups (P>0.05). Each replicate was placed in the same cage (0.9m x 0.6m). The basal diet adopts corn-soybean meal type diet, and is formulated into powdered compound diet with reference to "Standard for Chicken Feeding" (NY / T 33-2004). The control group was fed the basal diet, the hesperidin group was fed the basal diet + 60 mg / kg hesperidin preparation, the rosmarinic acid group was fed the basal diet + 30 mg / kg rosmarinic acid preparation, The hesperidin-rosmarinic acid gro...
Embodiment 3
[0048] Determination of growth performance indicators
[0049] At the beginning of the experiment, the initial body weight of 1-day-old broilers was weighed in each replicate and recorded. During the test period, at 21 and 42 days of age, the body weight of the broilers was measured with each repetition as a unit after fasting for 3 hours. The weight ratio is used to evaluate the effect of hesperidin-rosmarinic acid improving meat quality preparation on various growth performance indicators of broilers. The results are shown in Table 1.
[0050] Table 1 Effects of hesperidin preparations, rosmarinic acid preparations and hesperidin-rosmarinic acid preparations for improving meat quality in diets on the growth performance of broilers
[0051]
[0052] The growth performance results of broilers in the control group, hesperidin group, rosmarinic acid group, and hesperidin-rosmarinic acid group are shown in Table 1: Compared with the control group, the rosmarinic acid preparat...
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