Ultrasonic and near-infrared light excited phase-change magnetic nano thrombolytic drug and preparation method thereof

A near-infrared light, magnetic nanotechnology, applied in the fields of drug combination, drug delivery, pharmaceutical formulation, etc., can solve the problems of lack of targeting, unable to quickly clear large thrombus at a fixed point, etc., and achieve long retention time, excellent photothermal effect, The effect of improving the efficiency of thrombolysis

Pending Publication Date: 2022-07-29
FUZHOU UNIV +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the prepared thrombus-targeted long-circulation slow-release liposome overcomes the problems of current thrombus drugs that require a large amount of frequent administration and lack of targeting, as a sustained-release drug, it cannot quickly remove large thrombus at a fixed point, which limits its clinical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ultrasonic and near-infrared light excited phase-change magnetic nano thrombolytic drug and preparation method thereof
  • Ultrasonic and near-infrared light excited phase-change magnetic nano thrombolytic drug and preparation method thereof
  • Ultrasonic and near-infrared light excited phase-change magnetic nano thrombolytic drug and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] The invention discloses a phase-change magnetic nano-thrombotic drug excited by ultrasound and near-infrared light and a preparation method thereof, which mainly include the following steps:

[0033] (1) The nanoscale Fe 3 O 4 Dispersed in methanol and pretreated with ultrasonic;

[0034] (2) Dissolve DPPC, DPPA, DPPE and DSPE-mPEG-2000 in chloroform;

[0035] (3) Nanoscale Fe dispersed in methanol obtained in step (1) 3 O 4 adding into the chloroform solution obtained in step (2), and evaporating the solvent with a rotary evaporator to form a uniform lipid film on the inner surface of the rotary evaporator;

[0036] (4) Prepare a hydration solution, then add the hydration solution into the rotary evaporation bottle in step (3), and ultrasonically peel off the lipid film and disperse it in the hydration solution;

[0037] (5) The hydration solution with dispersed lipid film obtained in step (4) is crushed with a cell crusher, and PFH liquid is added dropwise during...

Embodiment 1

[0043] (1) 10 mg Fe 3 O 4 Disperse in 5 mL methanol and sonicate for 20 min.

[0044] (2) DPPC, DPPA, DPPE, DSPE-mPEG-2000 were weighed and dissolved in 10 mL of chloroform, respectively weighing 20 mg: 6 mg: 6 mg: 6 mg.

[0045] (3) Nanoscale Fe dispersed in methanol obtained in step (1) 3 O 4 It is added to the chloroform solution obtained in step (2), and the solvent is evaporated to dryness with a rotary evaporator to form a uniform lipid film on the inner surface of the rotary evaporator. The rotary evaporation operation was performed with a temperature of 55 °C, a vacuum of 200 bar, a rotation speed of 300 rpm, and a rotary evaporation time of 30 min.

[0046] (4) The hydration solution was prepared, and the components of the hydration solution were PBS buffer solution, glycerol and block polyether F-68 and UK. The formulated quantities were 5 mL, 0.5 mL, 3 mg, and 20 mg, respectively. Then add the hydration solution into the rotary evaporation bottle in step (3), ...

Embodiment 2

[0051] (1) 5 mg Fe 3 O 4 Disperse in 5 mL methanol and sonicate for 20 min.

[0052] (2) DPPC, DPPA, DPPE, DSPE-mPEG-2000 were weighed and dissolved in 10 mL of chloroform, respectively weighing 20 mg: 6 mg: 6 mg: 6 mg.

[0053] (3) Nanoscale Fe dispersed in methanol obtained in step (1) 3 O 4 It is added to the chloroform solution obtained in step (2), and the solvent is evaporated to dryness with a rotary evaporator to form a uniform lipid film on the inner surface of the rotary evaporator. The rotary evaporation operation was performed with a temperature of 55 °C, a vacuum of 200 bar, a rotation speed of 300 rpm, and a rotary evaporation time of 30 min.

[0054] (4) The hydration solution was prepared, and the components of the hydration solution were PBS buffer solution, glycerol and block polyether F-68 and UK. The formulated quantities were 5 mL, 0.5 mL, 3 mg, and 20 mg, respectively. Then add the hydration solution into the rotary evaporation bottle in step (3), a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an ultrasonic and near-infrared light excited phase-change magnetic nano thrombolytic drug and a preparation method thereof. An artificial blood material PFH is used as a near-infrared light and ultrasonic excited phase-change initiator and an ultrasonic and photoacoustic developer to be self-assembled in a magnetic nano liposome to be applied to the field of thrombus treatment. Under the conditions of 808 nm near-infrared laser and focused ultrasound, PFH is rapidly vaporized and cracks lipidosome, urokinase in the content is released and cooperates with bubbles generated by phase change of PFH to rapidly penetrate a suppository block under the cavitation effect of ultrasound. A thrombus treatment process is simulated in an in-vitro static and dynamic blood vessel model and a femoral vein thrombus model of an SD rat, and under the action of laser and ultrasound, nano particles can efficiently dissolve blood clots. The thrombolytic drug disclosed by the invention not only has thrombolytic capacity, but also has ultrasonic imaging and photoacoustic imaging functions and magnetic targeting capacity, and the risk that in-vivo bleeding complications are easily caused by conventional urokinase drug treatment is greatly avoided.

Description

technical field [0001] The invention belongs to the technical field of medical materials, in particular to a phase-change magnetic nano-thrombotic drug excited by ultrasound and near-infrared light and a preparation method thereof. Background technique [0002] At present, venous thromboembolism has become a major burden on the global medical system, with approximately 10 million cases per year, and is the most common cardiovascular disease after stroke and acute myocardial infarction. Survivors of deep vein thrombosis have a poor prognosis, with a mortality rate of approximately 10% per year. More than 50% of patients with deep vein thrombosis will develop post-thrombotic syndrome, which is characterized by pain, edema, and ulcers at the thrombus site, which seriously reduces the patient's quality of life. Deep vein thrombosis is the third leading cause of death from cardiovascular disease worldwide, after coronary artery disease and stroke. [0003] Currently clinical th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/127A61K47/24A61P7/02A61K49/22A61K49/18
CPCA61K41/0052A61K9/1271A61K47/24A61P7/02A61K49/227A61K49/1812A61K49/0002
Inventor 郑辉东刘宁张进阮任杰
Owner FUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap