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Alfalfa endophytic pediococcus pentosaceus EL5 and application thereof

A technology of Pediococcus pentosaceus and alfalfa, applied in the application, bacteria, microorganism and other directions, can solve the problems of poor adaptability, low activity, restricting alfalfa silage, etc., and achieves the effects of low cost, easy utilization, and improved fermentation quality

Active Publication Date: 2022-08-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mutual selection and co-evolution between microorganisms and their living environment. At present, conventional exogenous lactic acid bacteria additives have low activity due to poor adaptability to raw materials during alfalfa fermentation, which is an important factor restricting alfalfa silage

Method used

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  • Alfalfa endophytic pediococcus pentosaceus EL5 and application thereof
  • Alfalfa endophytic pediococcus pentosaceus EL5 and application thereof
  • Alfalfa endophytic pediococcus pentosaceus EL5 and application thereof

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preparation example Construction

[0036] An embodiment of the present invention provides a method for preparing silage, comprising: mixing the silage raw material with the above-mentioned Pediococcus pentosaceus EL5 and fermenting to obtain the silage.

[0037] In one embodiment, the silage material can be alfalfa, and further can be alfalfa.

[0038] In the silage of one embodiment, the ratio of the viable count of Pediococcus pentosaceus EL5 to the mass of the silage material is 1.0×10 5 CFU / g~2.0×10 6 CFU / g, e.g. 2.0×10 5 CFU / g, 5.0×10 5 CFU / g, 8.0×10 5 CFU / g, 1.0×10 6 CFU / g, 1.5×10 6 CFU / g.

[0039] A method for preparing silage according to an embodiment of the present invention includes the following steps:

[0040] S1: Chop the alfalfa raw materials and mix them evenly;

[0041] S2: Add Pediococcus pentosaceus EL5 to the alfalfa raw material, and the effective viable count of Pediococcus pentosaceus EL5 per gram of alfalfa raw material is 1.0×10 6 more than one.

[0042] S3: The alfalfa system...

Embodiment

[0054] Isolation and culture of endophytic Pediococcus pentosaceus EL5

[0055] Put the fresh alfalfa material in a sterile sampling bag and bring it back to the laboratory. Weigh 10g of the whole alfalfa plant in the sterile environment of the ultra-clean workbench and then carry out surface disinfection operation: immerse it in 70% ethanol for 90s, 3.25 ~4% sodium hypochlorite solution for 120s, 70% ethanol for 30s, then rinsed with sterile water for 3 times and used sterile filter paper to absorb the surface moisture, and the sterile water of the last rinse was applied as a control.

[0056] Put the sterilized material into a sterilized mortar for grinding (add a little sterilized quartz sand to help grinding, and calcium carbonate as a buffer), grind the alfalfa tissue, add 9 mL of 8.5% sterile physiological saline and stir well, Then let stand for 3min, take the supernatant (recorded as 10 -1 Concentration gradient) 1mL and successively diluted to 10 by 10-fold dilution ...

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PUM

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Abstract

The invention relates to pediococcus pentosaceus and an application of the pediococcus pentosaceus. The pediococcus pentosaceus is pediococcus pentosaceus EL5 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.23923. The invention also relates to a preparation method of the pediococcus pentosaceus. The pediococcus pentosaceus EL5 provided by one embodiment of the invention is used for silage, and can improve the activity of lactic acid bacteria and improve the quality of silage fermentation.

Description

technical field [0001] The present invention relates to microbial feed, in particular to a kind of alfalfa endophytic Pediococcus pentosaceus EL5 suitable for preparing silage, especially alfalfa silage. Background technique [0002] Alfalfa is a perennial herb of the genus Alfalfa in the leguminous family. It is resistant to drought and is mainly distributed in Northwest my country, North China, Northeast China and the Jianghuai River Basin. It has a cultivation history of more than 2,000 years in my country. Alfalfa has the advantages of high protein content and good palatability, and is a high-quality forage source for ruminants, especially dairy cows. The demand for alfalfa forage in my country's animal husbandry industry is increasing day by day, and it relies heavily on imports from abroad. The contradiction between supply and demand is increasingly prominent, which seriously restricts the development of my country's animal husbandry industry. [0003] Silage, as a si...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18C12R1/01
CPCC12N1/20A23K30/18A23V2400/427Y02P60/87A23K10/12C12N1/205C12R2001/01
Inventor 倪奎奎周鸿章杨富裕
Owner CHINA AGRI UNIV