Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Electro ultrafiltration liquation crystallization separation purification method of biomacromolecule

A technology of biological macromolecules and elution crystallization, which is applied in the field of electro-ultrafiltration of biological macromolecules - elution crystallization separation and purification method, can solve problems such as limited operation units, and achieves inhibition of concentration polarization, continuous separation and purification, The effect of improving flow characteristics

Inactive Publication Date: 2004-12-15
SOUTH CHINA UNIV OF TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the crystallization of biomacromolecules is still a research hotspot. Due to technical and economic reasons, the applicable operating units for the separation and purification of biomacromolecules are quite limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Electric ultrafiltration-dissolution crystallization method is used for the separation and purification of dextran 20 crude product

[0026] The first step is the primary separation of the raw material liquid

[0027] Get the supernatant after centrifuge separation, centrifuge speed is 3000r / min, centrifugation time is 15min;

[0028] The second step is to separate and concentrate the material under the action of an external electric field

[0029] The main reaction parameters are: select the ultrafiltration membrane with a molecular weight cut-off of 10kD, apply a DC electric field voltage of 5V, operate at a temperature of 25°C, and operate at a pressure of 0.05Mpa;

[0030] The third step is to refine by coupling electric ultrafiltration and elution crystallization

[0031] The organic solvent is ethanol, and the solid content of the concentrated solution is 10% (W / V) when adding ethanol, and the concentration ratio of the feed liquid (total volume of the...

Embodiment 2

[0036] Example 2 Electric ultrafiltration-dissolution crystallization method is used for the separation and purification of dextran 40

[0037] The first step is the primary separation of the raw material liquid

[0038] Sieve the raw material liquid with a 300-mesh wire mesh sieve, and get the filtrate;

[0039] The second step is to separate and concentrate the material under the action of an external electric field

[0040] The main reaction parameters are: select ultrafiltration membrane with a molecular weight cut-off of 20kD, apply a DC voltage of 50V, operate at a temperature of 45°C, and operate at a pressure of 0.20Mpa;

[0041] The third step is to refine by coupling electric ultrafiltration and elution crystallization

[0042]Organic solvent is ethanol, and the solid matter concentration of concentrated solution is 18% (W / V) when adding ethanol, and the concentration ratio of feed liquid (the total volume of raw material liquid / retained liquid volume) is 5, and th...

Embodiment 3

[0047] The refining of embodiment three dextran 10

[0048] The treatment object is the by-product (permeate of 10kD ultrafiltration membrane) obtained after producing dextran 40 and dextran 20. Small molecule dextran can be used as a food preservation agent and a protective agent for clinical organ transplantation. However, it is difficult to prepare by the traditional ethanol fractional precipitation method.

[0049] The first step is the primary separation of the raw material liquid

[0050] Use a 10kD ultrafiltration membrane to separate and take the filtrate;

[0051] The second step is to separate and concentrate the material under the action of an external electric field

[0052] The main reaction parameters are: select the ultrafiltration membrane with a molecular weight cut-off of 5kD, apply a DC electric field voltage of 20V, operate at a temperature of 5°C, and operate at a pressure of 0.30Mpa;

[0053] The third step is to refine by coupling electric ultrafiltr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

An electric ultrafilter-solution educing crystallization method for separating and purifying biomacromoleculae includes such steps as removing suspended particles from raw liquid, applying external electric field, separation and concentrating, refining by coupling the electric ultrafilter with solution educing crystallization, and educting out crystal from the concentrated liquid in a crystallizer. Its advantages are high purity, long service life of ultrafilter, low solvent consumption and short period.

Description

(1) Technical field [0001] The invention relates to the technical field of separation and purification of biological macromolecules such as polysaccharides and proteins, and specifically refers to an electric ultrafiltration-dissolution crystallization separation and purification method for biological macromolecules. (2) Background technology [0002] Biomacromolecules are generally separated from fermentation broth or enzyme reaction fluid or animal and plant cells. Compared with general chemicals, the separation and purification of biomacromolecules has the following characteristics: 1. In raw materials such as fermentation broth and culture fluid The content of biological macromolecules in medium is generally low; 2. The raw material liquid is a mixture of multiple components with a very wide molecular weight distribution. 3. The stability of biological macromolecules is poor, the chemical properties are not stable enough, and they are easily denatured when exposed to hea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B01D9/02B01D57/00B01D61/14C07H1/06C07K1/34C07K1/36
Inventor 郭祀远陈山李琳蔡妙颜肖凯军郑必胜陈玲
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com