A bacterial strain and carotenoid production method using said strain
A technology of Escherichia and carotene, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of seasonal factors, unstable output, large amount of organic solvents, etc., and achieve the production process Simple, inexpensive to produce effect
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Embodiment 1
[0041] Example 1: The specific purification method of the Escherichia species of the present invention
[0042] For the soil at a depth of 5-6cm in the surface layer near N30°41.602’ and E111°18.499’, the specific non-restrictive separation steps are:
[0043] ①Prepare beef extract peptone solid medium, and pour the plate;
[0044] ②Take 10g of soil at a depth of 5-6cm in the surface layer near N30°41.602' and E111°18.499' to prepare a soil dilution solution to obtain 10-1, 10-2, 10-3, 10-4, 10-5, 10 -6 soil solutions with different dilutions;
[0045] ③Spread the above-mentioned soil suspensions of different concentrations on the surface of the culture medium of the beef extract peptone plate using the plate coating method;
[0046] ④ Put the coated beef extract peptone plate upside down in a 37°C incubator for 24 hours at a constant temperature;
[0047] ⑤Single colony that produces red pigment that grows after culture, the characteristics of the colony: the colony is smooth, mo...
Embodiment 2
[0050] Example 2: Method for using the Escherichia species to produce carotenoids
[0051]Add 8% by mass of corn flour, 0.5% by mass of rice bran, 0.02% by mass of dipotassium hydrogen phosphate, and 0.01% by mass of sodium chloride into 100ml of distilled water and mix at 121°C Sterilize for 20 minutes, cool to room temperature, add 0.1% by mass high-temperature α-amylase, liquefy for 1 hour, cool to room temperature, then add 0.1% by mass glucoamylase for saccharification, and the access mass percentage is 5% Escherichia spp., adjust the fermentation broth with alkaline substances to make the pH of the fermentation broth 5.5, and then aerate and cultivate at a temperature of 28°C-30°C for 48 hours, and finally take the fermentation broth. The floating substance is detected, filtered, extracted, and crystallized to obtain a solid carotenoid with a mass percentage of 5.4% and a purity of 97%. This product is red.
Embodiment 3
[0052] Example 3: Method for producing carotenoids by using the Escherichia species
[0053] Add 8% by mass corn starch, 0.5% by mass bran, 0.02% by mass dipotassium hydrogen phosphate, and 0.01% by mass sodium chloride into 100ml of distilled water and mix at 121℃. Sterilize for 20 minutes, cool to room temperature, add 0.1% by mass high-temperature α-amylase, liquefy for 1 hour, cool to room temperature, and then add 0.1% by mass glucoamylase for saccharification, with a mass percentage of 5 % Of Escherichia strains, adjust the fermentation broth with alkaline substances to make the fermentation broth pH 5.5, and then aerate and cultivate at a temperature of 28℃-30℃ for 48 hours, and finally take the fermentation broth The floating substance is detected, filtered, extracted, and crystallized to obtain a solid carotenoid with a mass percentage of 5.4% and a purity of 97%. This product is red.
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