Method for discovering wild rice beneficial gene using convertible large fragment genome library

Abstract

The invention discloses a method for exploring wild rice advantageous gene using transformable large fragment genome library, the steps including: constructing wild rice transformable large fragment genome library, inducing large fragment clone into cultivated rice through transgene technology, establishing full genome gene knock in mutant library, after field screening about character and genetic model controlled by large fragment clone in mutant, obtaining gene transferred mutant rice dramatically improved in yield ,resistance and quality etc. Once large fragment clone containing advantageous gene is authenticated, it can be utilized in other cultivated rice variety, which modifies the existing rice varieties and can be the same with exploring other plant gene resource.

Description

technical field [0001] The invention belongs to the technical field of rice variety improvement and breeding. It involves the field of molecular biology technology and genetic engineering technology for large-scale excavation and utilization of favorable genetic resources of wild rice. Background technique [0002] Wild rice contains rich beneficial genes. The successful excavation and utilization of wild rice genetic resources have brought about the world's first and second green revolutions. The transfer of dwarf genes from wild rice to cultivated rice brought about the first green revolution; hybrid rice is known as the fifth greatest invention in my country. More than 30 years ago, Academician Yuan Longping first discovered wild rice from wild rice. Yu Gene successfully bred hybrid rice and created the second green revolution. Because wild rice has been in the wild for a long time and has been selected by various disasters and adverse environmen...

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Problems solved by technology

[0004] At the same time, there are many limitations in the existing utilization technology of favorable genes in wild rice

For example, the hybrid transgenic offspring of wild rice and cultivated rice have been segregated wildly for a long time, the stability is slow, and the breeding cycle is long; methods such as embryo rescue, tissue culture, and protoplast fusion are mainly aimed at wild rice with non-AA karyotypes. It is difficult to obtain wild rice, and at the same time, it is impossible to avoid the crazy segregation of offspring; it is difficult to easily and quickly introduce and reproduce the beneficial genes of wild rice in multiple cultivated rice varieties; Stability, or the introduction of some genes that control the target trait

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