Method for cultivating nobal metal enriched transgenic plant by total

Abstract

The method for breeding heavy metal enriched transgenic plant by utilizing total DNA leading in technology includes the following steps: selecting verified heavy metal superenriched plant a donor plant, using the plant with large biomass, rapid growth speed and extensive adaptability as receptor plant, using CTAB method to extract total DNA of donor plant, utilziing improved total DNA leading in technique to introduce the donor plant DNA into the immature ovary of receptor plant or its embryo, sowing the seed into the culture medium or soil in which the heavy metal content is reached to the concentration of prototype plant growth before inhibition treatment and screening and defining the plant whose heavy metal enriching capacity is raised by above 5 times as compared with that of prototype plant before transgene as heavy metal enriched transgenic plant.

Description

technical field [0001] The invention belongs to the technical fields of biotechnology and environmental protection. In particular, it relates to a method for cultivating heavy metal-enriching transgenic plants using total DNA introduction technology. Background technique [0002] 1. Metal pollution problem [0003] Heavy metal pollution has become a major environmental problem worldwide. Heavy metal pollution not only threatens the growth and development of animals, plants and microorganisms, but also threatens human health and even life through the food chain. Heavy metals that cause soil pollution mainly include As, Cd, Co, Cr, Cu, Hg, Mn, Ni, Pb, Zn, etc., which are generally compound pollution of several heavy metals. At present, the area of ​​cultivated land polluted by heavy metals in my country is nearly 20 million hectares, accounting for about 1 / 5 of the total cultivated land area, of which industrial "three wastes" pollute 10 mil...

AI Technical Summary

Problems solved by technology

[0008] 3. Limitations of Phytoremediation Technology

[0010] (1) There are few types of hyperaccumulator plants, usually short plants, low biomass, slow growth, and long growth cycle, so the restoration efficiency is low and it is not easy to mechanize operations

At present, there are more than 400 species of hyperaccumulator plants reported in the world, but 350 of them are Ni hyperaccumulators, only 1 species of As hyperaccumulators, and although there are 25 species of Cu hyperaccumulators, 24 of them are Ni hyperaccumulators. Distributed in Zaire, due to the war, it is no longer possible to conduct in-depth research and use

[0011] (2) A hyperaccumulator plant often only absorbs one or a few heavy metal elements, and shows some symptoms of poisoning to other heavy metals with higher concentrations in the soil, thus limiting the use of phytoremediation technology in soils contaminated by various heavy metals Governance Applications

The introduction of total DNA includes pollen tube passage method, ovary injection method, embryo injection method, seed soaking method, etc. The total DNA of different donors has been successfully introduced into tobacco, rice, cotton, wheat, pepper, etc., and corresponding transgenic plants have been obtained. However, so far there is no report of transgenic plants enriched or hyperaccumulated in heavy metals by total DNA introduction technology