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Streptomyce roseoviolaceus var. roseoruber var. sp. nov. and its preparation method

A technology of Streptomyces rosea and Streptomyces violaceum is applied in the fields of botanical equipment and methods, bacteria, and animal repellents, which can solve the problems of hampering the development of biological pesticides, unfavorable one drug for multiple treatments, and narrow insecticidal spectrum. , to ensure the increase of agricultural production and income and sustainable development, improve biodiversity, and improve the effect of protecting the environment

Inactive Publication Date: 2003-09-24
WUHAN EAST LAKE HIGH TECH GRP CO LTD
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  • Summary
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AI Technical Summary

Problems solved by technology

[0005] Bio-derived pesticides have been fully developed and widely used, but there are still some outstanding problems and difficulties in their research, development, production and application, which hinder the development of bio-derived pesticides in China
For example, the knockdown speed of biological source pesticides is slow, the validity period is short, and the relative insecticidal spectrum is relatively narrow. The control of pest populations is not strong in the short term, and the population density of pests cannot be quickly reduced.
Compared with the narrow insecticidal spectrum, it is not conducive to multi-treatment with one drug. For example, when multiple pests appear in the vegetable field, they will lose sight of the other and be unable to do what they want. It is difficult to apply and promote

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0073] 1. Isolation and cultivation of strains

[0074] Before separation, the soil samples were treated with high temperature dry heat at 120°C for one hour, and then two separation methods were used for pretreatment: 1) 6% yeast extract was added to 5umol phosphate buffer, and treated at 40°C for 20 minutes; 2) 6% Yeast extract, 0.1% SDS, 2mgNA (nalidixic acid) treatment. Nutrient agar was used as the separation medium, and 20ppm NA or 50ppm potassium dichromate was added as a selective inhibitor, cultured at 28°C, single colonies with special morphology and no pollution were selected for 3-7 days, and finally cultivated on No. 38 medium.

[0075] (1) Separation medium 1: soluble starch 1%, casein 0.03%, potassium nitrate 0.2%, magnesium sulfate heptahydrate 0.005%, sodium chloride 20%, dipotassium hydrogen phosphate 0.2%, calcium carbonate 0.002%, iron sulfate 0.001%, water 78%, pH7.0; culture temperature 28°C.

[0076] (2) Isolation medium 2 (ISP5): L-asparagine 0.1%, gl...

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Abstract

The present invention relates to a streptomyce roseoviolaceus var. roseoruber var. sp. nov. CCTCC No.M201030 and its preparation method. Its preparation method includes the following steps: drying and pretreating soil sample, using sterile water to dilute concentration, coating on separation culture meidum, inverted culture in calorstat, selecting single colony, culture on culture medium, shake-flask fermentation of separated pure strain, using fermented broth water-phase and fat phase sample to make biological screening, mixing the water-phase, artificial feed and target insect ovum and making mixed culture. Said ivnention has high activity for killing insects, can be used for killing bollworm, asparagus fern caterpillar, cabbageworm, diamondback moth and pine moth, etc.

Description

technical field [0001] The invention relates to Streptomycerosoviolaceus var. roseoruber var.sp.nov. having broad-spectrum insecticidal activity, and also relates to a preparation method of the Streptomycerosoviolaceus var.roseoruber var.sp.nov. Background technique [0002] With the wide application of chemical pesticides in the control of crop diseases and insect pests, the environmental pollution and public hazards caused by them are also becoming more and more serious. Moreover, the accumulation of pesticides in the food chain caused by chemical pesticide residues is not easy to control, resulting in a large number of drug-induced diseases, reducing people's quality of life and damaging the health of the people. In terms of earning foreign exchange through agricultural exports, pesticide residues have also reduced the competitiveness of my country's agricultural and sideline products. At the same time, the use of a large number of chemical pest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C12N1/20
Inventor 谢天键刘华梅杨和文张述谢翔季琦晔
Owner WUHAN EAST LAKE HIGH TECH GRP CO LTD