Process for extracting, preparing and purifying gamma methyllinolenate from algae

Abstract

Algae powder is added into methanol as entainer, the mixture is homogenated and supercritical extracted with CO2 as solvent and general ester is obtained after concentration. The general ester is dissolved and esterified in methanol containing acetyl chloride to obtain general fatty methyl ester, which is distilled with the distillate being collected. The distillate is mixed with acetone, crystallized in 4 deg.c, -10 deg.c and -30 deg.c successively, filtered to eliminate separated matter, the filtrate is concentrated and mixed with urea and methanol, and the mixture is reflux for 1 hr under the protection of nitrogen, cooled, set, filtered to eliminate crystal, mixed with water, petroleum ether extracted to obtain gamma-methyl linolenate after petroleum ether is recovered via distillation. The obtained gamma-methyl linolenate is purified via high vacuum distillation at 100-150 deg.c.

Description

1. Technical field [0001] The invention relates to gamma-linolenic acid methyl ester, in particular to the process of extracting, preparing and purifying gamma-linolenic acid methyl ester from algae. 2. Background technology [0002] γ-linolenic acid (GLA) and its derivatives are important anti-tumor active substances, and also have a variety of potential therapeutic effects on cardiovascular and cerebrovascular diseases, so the separation and purification of GLA has attracted attention at home and abroad. At present, it is generally used to extract from evening primrose seed oil (containing about 7% GLA), but because GLA is easily oxidized and there are a large number of compounds with similar physical properties to GLA, such as linoleic acid, etc., the separation and purification process of GLA is very difficult. , the cost is huge, and there has been no suitable process that can be applied on an industrial scale. [0003] Existing separation techniques such as preparativ...

AI Technical Summary

Problems solved by technology

At present, it is generally used to extract from evening primrose seed oil (containing about 7% GLA), but because GLA is easily oxidized and there are a large number of compounds with similar physical properties to GLA, such as linoleic acid, etc., the separation and purification process of GLA is very difficult. , the cost is huge, and there has been no suitable process that can be applied on an industrial scale

[0003] Existing separation processes such as preparative gas chromatography, enzymatic directional hydrolysis, and chromatography are all inefficient and costly and cannot be applied to industrial production.

There are related reports using a supercritical extraction, urea inclusion, or molecular distillation methods alone, but due to the nature of GLA, any separation method alone cannot obtain satisfactory results, or the final purity is too low , or the cost is too high

Extracting GLA from Spirulina has been reported to use organic solvent extraction-column chromatography, but there are also problems of high cost and low purity.