Process for preparing gelatin from enzyme degradation bone collagen

A collagen and enzymatic degradation technology, applied in the field of gelatin preparation, can solve the problems of long production cycle, large water consumption, environmental pollution in surrounding areas, etc., and achieve the effects of reducing electricity consumption, shortening production cycle and improving economic benefits.

Inactive Publication Date: 2004-02-11
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are always three major insurmountable defects in the preparation of gelatin by the traditional alkaline process: first, the production cycle is long, ranging from 60 to 100 days depending on the season, climate and region
The great disadvantage brought by the long production cycle is that the production efficiency is extremely low, especially the quality of gelatin produced between different batches cannot be guaranteed to be unchanged
Second, the water consumpt...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1). Select degreasing non-demineralized bovine bone hard aggregate and add water to mix and grind into bovine bone slurry. The bone particle size of the bovine bone slurry is controlled at 1mm;

[0022] (2). The bovine bone slurry in the reaction pot is adjusted to a pH value of 2 with hydrochloric acid, and then pepsin is added to control collagen degradation, wherein the amount of protease added is 4‰ of the weight of the bovine bone slurry;

[0023] (3). React at room temperature for 6 hours, then use calcium hydroxide to adjust the pH of the reaction solution to 5.0, heat the temperature to 70 to 85°C for extraction, and extract once for every 5°C increase in temperature. Extract 4 times;

[0024] (4). The turbid gelatin solution obtained by extraction is separated from the bone residue of coarse particles with a centrifuge, and the separated bone residue is returned to the reaction pot to extract the gelatin solution again;

[0025] (5). The isolated glue solutio...

Embodiment 2

[0027] (1). Select degreased and non-demineralized bovine offal bone and sponge aggregate and add water to mix and grind into bovine bone slurry. The bone particle size of the bovine bone slurry is controlled at 1mm;

[0028] (2). The bovine bone slurry in the reaction pot is adjusted to a pH value of 7.5 with sodium hydroxide, and then trypsin is added to control collagen degradation, wherein the amount of trypsin added is 5‰ of the weight of the bovine bone slurry;

[0029] (3).React at room temperature 25°C for 7 hours, then use hydrochloric acid to adjust the pH of the reaction solution to 6.5, heat the temperature to 70 to 85°C for extraction, and extract once for every 5°C increase in temperature, and extract together 4 times;

[0030] (4). Use a centrifuge to remove the coarse grained bovine bone residue from the turbid gelatin solution obtained by extraction, and then return the separated bovine bone residue to the reaction pot to re-extract the gelatin solution.

[0...

Embodiment 3

[0042] (1). Select degreasing non-demineralized cattle, horses and pigs hard aggregates and mix them with water to grind them into bone slurry of cattle, horses and pigs. The bone size of the bone slurry of cattle, horses and pigs is controlled at 5mm;

[0043] (2). The bone mud of cattle, horses and pigs in the reaction pot is adjusted to a pH value of 3 with hydrochloric acid, and then pepsin is added to control collagen degradation, wherein the amount of protease added is the weight of the bone mud of cattle, horses and pigs 7‰;

[0044] (3). React for 9 hours at room temperature at 25°C, then adjust the pH of the reaction solution to 5.5 with ammonium hydroxide, and extract when the temperature is heated to 70 to 85°C;

[0045] (4). The turbid gelatin solution obtained by extraction is separated from the bone residue of coarse particles with a centrifuge, and the separated bone residue is returned to the reaction pot to extract the gelatin solution again;

[0046] (5). Th...

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PUM

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Abstract

The present invention relates to gelatin preparation, and is especially the enzyme degradation process of preparing gelatin with bone collagen. The process includes milling the mixing of defatted bone material and water into bone slurry with bone size of 1-5 mm; regulating pH value to 1.5-4 with acid or to 7-8 with alkali and adding acid or alkali proteinase in 0.2-0.8 wt% of bone slurry to control the degradation of collagen; reaction at room temperature for 7-10 hr, regulating pH 5.0-6.5 with alkali or acid and heating to 70-85 deg.c for extraction; separating the turbid gelatin obtained via extraction; filtering gelatin liquid to obtain high purity hard capsule gelatin solution. The dried gelatin has molecular weight distribution of alpha=48 % and beta=18 %; gelatin jelly strength over 240 g; viscosity 3.0 mPs; transparency 90 % at 620 nm and 71 % at 450 nm.

Description

technical field [0001] The invention belongs to the field of gelatin preparation, and in particular relates to a method for preparing gelatin by enzymatically degrading bone collagen to produce high-purity, high-grade gelatin suitable for producing hard capsules. Background technique [0002] Gelatin is a protein extracted from the connective tissue (skin and bone) of animals (cattle, horse, pig) through a multi-step long degradation. The gelatin industry has a history of more than 100 years, and has been extracting bone gelatin from bone collagen by using the traditional alkaline process. The biggest advantage of the traditional alkaline process to prepare gelatin is: simple operation, separate product quality grades, the highest quality photographic glue can be obtained, and a certain proportion of industrial glue can also be produced. However, there are always three major insurmountable defects in the preparation of gelatin by the traditional alkali...

Claims

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Application Information

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IPC IPC(8): C09H1/02C09H3/00
Inventor 陈丽娟王颖史京京郭玉龙郑彤彭必先
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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