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Cellulase solid fermentation large-scale production method and equipment thereof

A technology of solid fermentation and production method, applied in directions such as hydrolase, can solve problems such as easy pollution of solid fermentation, and achieve the effect of high production yield

Inactive Publication Date: 2004-09-15
陈欣
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Problems solved by technology

[0004] The purpose of the present invention is to provide a large-scale production technology of cellulase solid fermentation, solve the technical problem that solid fermentation is easy to pollute, and can achieve high output, high quality and low price

Method used

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Embodiment Construction

[0024] The production steps are as follows:

[0025] 1. Selection of strains and subculture: Cellulase-producing engineered bacteria, Trichoderma sp., were obtained from the Institute of Microbiology, Chinese Academy of Sciences, Beijing, number: As3.3030.

[0026] The fermentation activity is more than 1000 units / gram of straw; through conventional strain test tubes, shaking flask expansion, and fermentation tank expansion until the required amount of liquid seeds;

[0027] Medium formula: Potato medium is used for the strain slope, and the proportion of the medium formula is: 200 grams of potato, 10 grams of glucose, 18 grams of agar, 3 grams of potassium dihydrogen phosphate, and 1.5 grams of magnesium sulfate heptahydrate per liter of culture solution 10 grams of filter paper powder, add water to 1000 ml;

[0028] 2. Culture conditions: a. Shake flask. When inoculating, scrape the mycelium into the shake flask with an inoculation loop, and cultivate it on a constant tempe...

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Abstract

The invention is a kind of large-scale producing method for cellulose solid yeast, it uses green trichoderma project strain, it is incubated through fungus cant tube, shaking bottle, the yeasting pot is expanded to the quantity of seeds needed by liquid, then carries on solid yeasting, they are transmitted to the asepsis inoculation room through asepsis channel, then they are placed in the plastic plate to be inoculated with incubation media, incubates for 72 hours, produces primary enzyme, condensates the liquid, and produces it into liquid enzyme through adding in sodium benzoate, the liquid enzyme is filtered and condensed adds in white ammonium sulfate, they are dried and produced into powder industry enzyme. The merit of the invention is that it realizes the solid yeasting large-scale producing, it has not contamination, the quality is high, the price is low.

Description

technical field [0001] The invention relates to a cellulase solid fermentation method. Background technique [0002] Cellulase is an enzyme that hydrolyzes plant fibers. It consists of C-1 enzyme, C-x enzyme and β-glucosidase. It can gradually degrade the cellulose in the plant cell wall until it finally decomposes to generate glucose. Therefore, cellulase is widely used in many processing and manufacturing industries, such as food, beverage, feed, wine making, fermentation, paper making, textile post-treatment, extraction of active ingredients of Chinese herbal medicine, oil exploration, sewage treatment and other fields, all of which are of high quality and high yield after use . In the research work of plant biotechnology, cellulase is often used to hydrolyze the cell wall for genetic engineering and cell engineering operations. So cellulase is known as the golden key to open the treasure house of plants. Cellulase currently only has a few small workshop-style producti...

Claims

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Application Information

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IPC IPC(8): C12N9/24
Inventor 陈欣陈红漫陈祖洁曹淡君王元志
Owner 陈欣
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