Cellulase solid fermentation large-scale production method and equipment thereof
A technology of solid fermentation and production method, applied in directions such as hydrolase, can solve problems such as easy pollution of solid fermentation, and achieve the effect of high production yield
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[0024] The production steps are as follows:
[0025] 1. Selection of bacterial species and subculture: cellulase-producing engineering bacteria, Trichoderma sp., taken from the Institute of Microbiology, Chinese Academy of Sciences, Beijing, number: As3.3030.
[0026] Fermentation activity is more than 1000 units / g straw; after conventional strain slant test tube, shake flask expansion, and fermenter expansion tank until the required amount of liquid seeds;
[0027] Medium formula: potato medium is used for the slant of the strain, and the medium formula ratio is: each liter of culture solution contains 200 grams of potato, 10 grams of glucose, 18 grams of agar, 3 grams of potassium dihydrogen phosphate, and 1.5 grams of magnesium sulfate 7hydrate. , 10 grams of filter paper powder, add water to 1000 ml;
[0028] 2. Cultivation conditions: a, shake flask, use the inoculation loop to scrape the mycelium into the shake flask, and cultivate it on a constant temperature shaking w...
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