Preparation method of CdSe nanometer crystal composite liposome microcapsule bubble used for fluorescence immunity detection

Abstract

The present invention belongs to the field of nano material and biological technology. The composite nano CdSe liposome microvesicle for fluorescent immunoassay is prepared through three steps of: preparing nano oily CdSe semiconductor crystal, synthesizing composite nano oily crystal microvesicle, and electrostatically connecting microvesicle with biological protein via altering pH value to regulate surface potential. The present invention is mainly used in test paper strip for immunochromatographic detection of hepatitis B virus, pesticide residue in vegetable and drug detection. The lipophilic surface nano CdSe crystal is phase transferred to constitute biocompatibility.

Description

technical field

[0001] The invention belongs to the field of nanomaterials and biotechnology, and relates to nanobiotechnology, in particular to a method for preparing CdSe nanocrystal composite liposome microvesicles for fluorescence immunity detection. Background technique

[0002] For biological and biomedical detection, fluorescent nanocrystals are a fluorescent probe with potential application value. Traditional fluorescent probes generally use organic dyes, which have many disadvantages as biological detection probes. The main performance is that the half-peak width of the fluorescence spectrum emitted by the organic dye is about 3-5 times the half-peak width of the fluorescence spectrum emitted by the fluorescent nanocrystal, which will cause spectral overlap and affect the sensitivity of the detection. At the same time, the excitation wavelength of the organic dye It is narrower than fluorescent nanocrystals, which limits the range of excitation light sources, that i...

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