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Preparation method of CdSe nanometer crystal composite liposome microcapsule bubble used for fluorescence immunity detection

A technology of nanocrystal composite and fluorescent nanocrystal, which is applied in the field of nanomaterials and biology, and can solve problems such as limiting the application of nanocrystals

Inactive Publication Date: 2004-11-03
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

This also limits the application of nanocrystals

Method used

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  • Preparation method of CdSe nanometer crystal composite liposome microcapsule bubble used for fluorescence immunity detection
  • Preparation method of CdSe nanometer crystal composite liposome microcapsule bubble used for fluorescence immunity detection
  • Preparation method of CdSe nanometer crystal composite liposome microcapsule bubble used for fluorescence immunity detection

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Embodiment Construction

[0024] Select 99.9% CdO: 0.0128-0.0150g; Se powder: 0.079-0.158g; Zinc stearate: 0.114-0.328g

[0025]Between 140-1500C, CdO reacts with stearic acid to generate cadmium stearate, then the reaction container of the product is lowered to room temperature, and TOPO and HDA are added to the container at room temperature, and then the temperature of the reactor is lowered. Raise to 300-3200C, inject TOPSe into the system at this temperature, and quickly cool down after injection. The cooling range is 250-2800C. Different cooling times can obtain nanocrystals of different sizes, that is, nanocrystals with sizes ranging from 3-5nm . The fluorescent color of the crystal gradually red shifted with time, that is, gradually changed from green to red. Figure 1 shows the fluorescent spectra of different sizes synthesized under the above conditions. On the basis of synthesizing different CdSe nanocrystals, Zn is directly injected into the system, and Zn combines with excess Se on the sur...

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Abstract

The present invention belongs to the field of nano material and biological technology. The composite nano CdSe liposome microvesicle for fluorescent immunoassay is prepared through three steps of: preparing nano oily CdSe semiconductor crystal, synthesizing composite nano oily crystal microvesicle, and electrostatically connecting microvesicle with biological protein via altering pH value to regulate surface potential. The present invention is mainly used in test paper strip for immunochromatographic detection of hepatitis B virus, pesticide residue in vegetable and drug detection. The lipophilic surface nano CdSe crystal is phase transferred to constitute biocompatibility.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biotechnology, and relates to nanobiotechnology, in particular to a method for preparing CdSe nanocrystal composite liposome microvesicles for fluorescence immunity detection. Background technique [0002] For biological and biomedical detection, fluorescent nanocrystals are a fluorescent probe with potential application value. Traditional fluorescent probes generally use organic dyes, which have many disadvantages as biological detection probes. The main performance is that the half-peak width of the fluorescence spectrum emitted by the organic dye is about 3-5 times the half-peak width of the fluorescence spectrum emitted by the fluorescent nanocrystal, which will cause spectral overlap and affect the sensitivity of the detection. At the same time, the excitation wavelength of the organic dye It is narrower than fluorescent nanocrystals, which limits the range of excitation light sources, that i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C30B29/48G01N21/64G01N33/533G01N33/543G01N33/84
Inventor 冯力蕴单桂晔孔祥贵
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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